Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
105 Cards in this Set
- Front
- Back
|
BA stands for
|
Blood Agar
|
|
|
What catergory is BA under?
|
Differential
|
|
|
What is the purpose of BA?
|
to test for hemolytic acitvity
|
|
|
What does MAC stand for?
|
MacConkey Agar
|
|
|
What catergory is MAC under?
|
Differential and Selective
|
|
|
What is the purpose of MAC?
|
for isolation of Gram (-) Enterics
|
|
|
What does PEA stand for?
|
Phenylethyl Alchohol Agar
|
|
|
What catergory is PEA?
|
Selective
|
|
|
What is the puropse of PEA?
|
Isolation of Gram (+)
|
|
|
What does MSA stand for?
|
Mannitol Salt Agar
|
|
|
What cateogry is MSA under?
|
Seletive and differential
|
|
|
What is the purpose of MSA?
|
Isolate spahtyloccus species
|
|
|
What does PA stand for?
|
Pseudomonas Isolation Agar
|
|
|
What cateogry is PA under?
|
Selective
|
|
|
What is the purpose of PA?
|
isolation of pseudomonas species
|
|
|
What is Staphylococcus epidermidis? (3)
|
(1)on the skin (2)opportunistic (3) most strains produce a slime layer which may enalble them to attach to cetrain hopstial apparatuses during invasive procedures
|
|
|
NAME
can be found on the skin and is opportunistic |
Staphylococcus epidermidis
|
|
|
NAME
most strains produce a slime layer which may enalble them to attach to cetrain hopstial apparatuses during invasive procedures |
Staphylococcus epidermidis
|
|
|
What is Stahphylococcus aureus? (2)
|
(1)most common noscomial bacteria infection (2)causes food poisning, toxic shock, scalded skin syndrome, pinke boils etc
|
|
|
causes food poisning, toxic shock, scalded skin syndrome, pinke boils etc
|
Stahphylococcus aureus
|
|
|
NAME
is the most common bacteria nosocomial infection |
Stahphylococcus aureus
|
|
|
NAME
also known as GAS |
Streptococcus pyogenes
|
|
|
GAS stands for
|
Group A Streptococcus
|
|
|
Streptococcus pyogenes are also called ( ).
|
GAS
|
|
|
NAME
is the most common cause of pharyngitis, but can also cause acute rhematic fever, and acute glomerulonephritis |
Streptococcus pyogenes
|
|
|
What is Streptococcus pyogenes? (3)
|
(1)also called GAS (2)common cause of pharyngitis (3) can also cause acute rheumatic fever, and acute glomerrulonephritis
|
|
|
NAME
is the most common cause of pharyngitis |
Strepcoccus pygones
|
|
|
Pharyngitis is ( ).
|
Sore throat
|
|
|
Sore throat can also be refered to as ( ).
|
Pharyngitis
|
|
|
What is Pseudomonas aeruginoa? (4)
|
(1)Opportunitstic w phagocyte resistant slime layer (2) has a fruity odor (3) a blue green pigment (4) can cause eye and ear infections
|
|
|
NAME
Opportunitstic w phagocyte resistant slime layer |
Pseudomonas aeruginosa
|
|
|
NAME
is nosciminal and can cause eye and ear infections |
Pseudomonas aeruginosa
|
|
|
NAME
has a fruity odor and a blue green pigment |
Pseudomonas aeruginosa
|
|
|
What is Escherichia coli?
|
(1)common resistant of digestive tract (2) produces Vitamin K and B (3)is a indicator bacterium
|
|
|
What can Escherichia coli cause(3)?
|
(1)food poisning (2) Montezumas revenge (3) UTI
|
|
|
NAME
common resistant of digestive tract |
Escherichia coli
|
|
|
NAME
produces Vitamin K and B |
Escherichia coli
|
|
|
NAME
is a indicator bacterium |
Escherichia coli
|
|
|
NAME
usual is the cause of UTI, food poisning, and Montezuma's revenge |
Escherichia coli
|
|
|
Describe the Mircococcus gensis (3)
|
(1)Gram (+) (2)aerobic (3)nonpathogenic
|
|
|
What is Micrococcus Luteus?
|
appears yellow in culture
|
|
|
What is Micrococcus?
|
appears red in culture
|
|
|
NAME
is gram (+), aerobic, and nonpathogenic |
Micrococcus luteus and Micrococcus roseus
|
|
|
NAME
appears yellow in culture |
Micrococcus luteus
|
|
|
Are species of the genre Micrococcus pathogenic?
|
No
|
|
|
NAME
appears red in culture |
Micrococcus roseus
|
|
|
Where is the sharps container located?
|
in the sliver container at the end of each lab table
|
|
|
What is a nosocomial infection?
|
a hospital acquired infection
|
|
|
How do you label plates and tubes?
|
(1)Media (2)organism (3) Team (4)Date (5)Intials
|
|
|
Why do you label at the bottom of plates?
|
for safety reasonsn incase someone removes the top and it becomes lost
|
|
|
What is the differ btwn Antiseptic and Disinfectant?
|
Antiseptic kills all germs on living tissue and Disinfectant kills tissue on inmate objects
|
|
|
NAME
kills all germs on living tissue |
Antiseptic
|
|
|
NAME
is a hopstial acquried infection |
nosocomial infection
|
|
|
NAME
kills tissue on intimate objects |
Disinfectant
|
|
|
What is the general prepartation for tubes?
|
melt before pipetting and autoclaving
|
|
|
What is the general preparation for plates?
|
autoclave and then pour
|
|
|
For ( ). the general preparation involes melting before pipetting and autoclaving
|
tubes
|
|
|
For ( ), the general preparation involes melting before pipetting and autoclaving
|
tubes
|
|
|
NAME
Category is General (2) |
(1)TSA (2)TSB
|
|
|
NAME
Category is Selective |
PA
|
|
|
NAME
Category is Differential |
BA
|
|
|
NAME
Category is both Selective and Differential (2) |
(1)MSA (2)MAC
|
|
|
What are the main differnces btwn preparing for media for tubes and plates?
|
For the tubes-melt before pipetting and autoclaving.
For the plates-autoclave and then pour |
|
|
NAME
Category is Bichemical |
TGB
|
|
|
What is the Category of TSB?
|
General
|
|
|
What is the category of TSA?
|
General
|
|
|
What is the catergory of TGB?
|
Biochemical
|
|
|
What are the steps in preparing a smear?
|
(1)a drop of water (2)add organism (3)smear to the size of a quarter (4)air dry (5)heat fix
|
|
|
Where does the contaminated Lab material go?
|
in the Biohazardous Waste Disposal
|
|
|
Give one difference btwn Antiseptic and Disinfictant with one ex of each
|
Antispetic technique is used to keep tissue free of microbes and a ex is sterilizing medical equipment (2)Disinfectant is to clean the surface are and a ex is disinfectant used to kill microbes when there is a spill during lab
|
|
|
What are the steps used to clean up a contaminated spill? (4)
|
(1)put a paper towel over the spill (2)spray the paper towel w disinfectant (3) wait 20 min (4)clean the spill
|
|
|
Which method of sterlization is used to destroy endospores and what type of heat sterliation is used?
|
the autoclave is used at a temp of 121 C at 15 psi
|
|
|
If by any means while doing pratical your glass slide breaks where then where should u place it?
|
the sharps container
|
|
|
Which standard method is used in micro lab for preventing and avoiding contamination of cultures and who used it first?
|
(1)Joseph Lister (2) aseptic techinques such as washing your hands before surgury
|
|
|
What does read the meniscus mean?
|
read to the line lowest where the water appears to be
|
|
|
What does Tare the balance refer to?
|
making a 1 plus zero balance
|
|
|
A recipe has 30 grams/1 liters, and how many grams of 160 ml do you want.
|
4.8 grams
|
|
|
How do you solve recipe problems?
|
convert the give grams to mL then mutilpy by the other given
|
|
|
A recipe has 50 grams/1 Liter, how many grams of 350 mL are needed?
|
17.5 grams
|
|
|
What does BA stand for?
|
Blood Agar
|
|
|
What does MAC stand for?
|
MacConkey Agar
|
|
|
What does PA stand for?
|
Pseudomonas Isolation Agar
|
|
|
What does PEA stand for?
|
Phenylethyl Alcholol Agar
|
|
|
What does MSA stand for?
|
MAnnitol Salt Agar
|
|
|
What does TGB stand for?
|
Thioglycolate Broth
|
|
|
What does TSA stand for?
|
Trypitcase Soy Agar
|
|
|
What does TSB stand for?
|
Trypticase Soy Broth
|
|
|
What is the purpose of TGB?
|
growth of anaerobic microbes
|
|
|
What is the purpose of TSB?
|
the growth of a wide range of bacteria
|
|
|
What tools do you use to incolulte the media? (3)
|
(1)loop (2)needle (3) swab
|
|
|
Name two ways for incolution of media
|
(1)Agar slant (2)Agar plate
|
|
|
What is Agar slant?
|
a liquid broth placed in deep agar tubes
|
|
|
What is agar plate?
|
is a quadrant streak used for isolation or seperation of a mixed culture
|
|
|
What is Clostridium sporogenes? (4)
|
(1)anaerobic (2)needs a medium such as TGB (3) does not produce neurotoxins (4)can cause inststinal water loss and hemorrhaging by destroying instetinal epitheaial cells.
|
|
|
NAME
are anaerobic and needs a medium such as TGB |
Clostridium sporogenes
|
|
|
NAME
does not produce neurotoxins |
Clostridium sporogenes
|
|
|
NAME
can cause intestinal water loss and hemorrhaging by destroying inestinal epitheal cells |
Clostridium sporogenes
|
|
|
What are Bacillus megaterium?
(3) |
(1)aerobic (2) produce endospores (3) easy study under the light microscope
|
|
|
NAME
is areobic and produce endospores |
Bacillus megaterium
|
|
|
NAME
are easy to study under the light microscope |
Bacillus megaterium
|
|
|
Name two ocular adjustments you have to make in order to avoid eye strain and headache associated w prolonged microscopic use
|
adust the inerpulpllliary distance and the diapter reading to 65 to 40
|
|
|
Why are microbes hard to see in wet preparations?
|
bc they are moving around, making it harder to focus on one microbe for a long period of time
|
|
|
How do you achieve subdued illumination when examining wet mounts?
|
you would darken the background by using the condenser and the illuminator
|
|
|
What organs of locomotion do you find in each of the living protozoans?
|
(1)pseudopods (2)cilla (3)flagella
|
|
|
Why do you need to use oil when viewing through the 100X objective?
|
bc the light refracts the image to the viewer, the more power the objective lens has the easier you can view the image
|
