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17 Cards in this Set

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What does the "TSI" in TSI agar test stand for?
Triple sugar-iron
What sugars are contained within the media and the concentration for each?
Sucrose 1.0%, lactose 1.0%, glucose .1%
What indicator is used within the media?
Phenol Red
What is TSI agar test, test for?
Distinguishes amongst Enerobacter species & other gram negative bacilli within the intestinal tract
What type of gas is formed with certain types of bacteria?
Hydrogen Sulfide - H2S
What is the substrate used in the media to create hydrogen sulfide (H2S)
Sodium Thiosulfate
What indicator within the media shows that a bacteria produced hydrogen sulfide (H2S)?
Ferrous Sulfide
How does ferrous sulfide indicate hydrogen sulfide (H2S) has been produced?
It combines with the gas a precipitates leaving a black color.
How can it be determined that only glucose has been fermented in the media?
Red slant & yellow butt - slow growth
How can it be determined that lactose and sucrose was fermented in the media?
Yellow slant & yellow butt - rapid growth
How can it be determined that neither lactose, sucrose, or glucose was fermented in the media?
Red slant & red butt - no growth
What is the pH (acid, basic, or neutral) when the media turns black because of the hydrogen sulfide (H2S)?
Acid
What is the time period to observe the media after incubation?
18 to 24 hours
Why is it important to view/observe the media within the suggested time?
The carbohydrate nutrients will run out and the usage of peptones within the media will be used; this will turn the media alkaline because of the ammonia produced as an end result of peptonization
Which enteric bacteria produced acidic slant/butt and which of them produced hydrogen sulfide (H2S)?
Escherichia coli, Enterobacter aerogenes, and Proteus vulgaris

-Proteus vulgaris
Which of the enteric bacteria has a red slant/butt?
Alcaligenes faecalis, Pseudomonas aeruginosa
Out of the 10 main bacteria, which of them are enteric?
Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Alcaligenes faecalis, Pseudomonas aeruginosa