Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
17 Cards in this Set
- Front
- Back
|
What does the "TSI" in TSI agar test stand for?
|
Triple sugar-iron
|
|
|
What sugars are contained within the media and the concentration for each?
|
Sucrose 1.0%, lactose 1.0%, glucose .1%
|
|
|
What indicator is used within the media?
|
Phenol Red
|
|
|
What is TSI agar test, test for?
|
Distinguishes amongst Enerobacter species & other gram negative bacilli within the intestinal tract
|
|
|
What type of gas is formed with certain types of bacteria?
|
Hydrogen Sulfide - H2S
|
|
|
What is the substrate used in the media to create hydrogen sulfide (H2S)
|
Sodium Thiosulfate
|
|
|
What indicator within the media shows that a bacteria produced hydrogen sulfide (H2S)?
|
Ferrous Sulfide
|
|
|
How does ferrous sulfide indicate hydrogen sulfide (H2S) has been produced?
|
It combines with the gas a precipitates leaving a black color.
|
|
|
How can it be determined that only glucose has been fermented in the media?
|
Red slant & yellow butt - slow growth
|
|
|
How can it be determined that lactose and sucrose was fermented in the media?
|
Yellow slant & yellow butt - rapid growth
|
|
|
How can it be determined that neither lactose, sucrose, or glucose was fermented in the media?
|
Red slant & red butt - no growth
|
|
|
What is the pH (acid, basic, or neutral) when the media turns black because of the hydrogen sulfide (H2S)?
|
Acid
|
|
|
What is the time period to observe the media after incubation?
|
18 to 24 hours
|
|
|
Why is it important to view/observe the media within the suggested time?
|
The carbohydrate nutrients will run out and the usage of peptones within the media will be used; this will turn the media alkaline because of the ammonia produced as an end result of peptonization
|
|
|
Which enteric bacteria produced acidic slant/butt and which of them produced hydrogen sulfide (H2S)?
|
Escherichia coli, Enterobacter aerogenes, and Proteus vulgaris
-Proteus vulgaris |
|
|
Which of the enteric bacteria has a red slant/butt?
|
Alcaligenes faecalis, Pseudomonas aeruginosa
|
|
|
Out of the 10 main bacteria, which of them are enteric?
|
Escherichia coli, Enterobacter aerogenes, Proteus vulgaris, Alcaligenes faecalis, Pseudomonas aeruginosa
|
