Micro Lab

Front 1

The Enterobacteriacae are Gram _____ that grow in the intestinal tract of humans and other animals

Back 1

negative

Front 2

The Enterobacteriacae family include Klebsiella, Enterobacter, and _____ coli.

Back 2

Esherichia

Front 3

E Coli is known for fecal _____, but +++++ and Enterobacter are also fermenters but not known for ++++ contamination.

Back 3

contamination
Klebsiella
fecal

Front 4

The ____ tests can differentiate between Klebsiella and ______ and E Coli.

Back 4

Enterobacter

Front 5

The indole test uses ____ ____ deep tubes. Three tests: ____ production, hyrdogen sulfide, and motility. When Indole. Sim agar contains tryptophan. Bacteria with enzyme ________can break down amino acid tryptophane to ______. Indole reacts with Kovac's reagent to produce ____ complex.

Back 5

sim agar
indole
tryptophanase
indole
pink

Front 6

MR-VP media contain glucose and _____. All enteric bacteria oxidize ______. Both tests run _____

Back 6

peptone
glucose
together

Front 7

The Methyl Red Test produce large quanitities of ____. Not further metabolized and said to be _____ The test assess whether bacteria making several +++++

Back 7

acids
stable
enzymes

Front 8

For biochemical tests, must have a medium, substrate, endproduct or products, _____, and +++++

Back 8

enzyme
reagents

Front 9

In Voges- Proskauer, some organisms such as Klebsiella and Enterobacter intially produce acid from glucose metabolism but further metabolize the acid to produce _____ endproducts, such as_____, ethyl alcohol, and 2,3 butanediol. .

Back 9

neutral
acetonin

Front 10

Acetonin will react with Barritt's reagent A and Barrit's reagent B to produce ____ or ___ red color. 20-30min

Back 10

mahogony or burgundy

Front 11

agrarian society

Back 11

society based on farming

Hint 11

vocabulary

Front 12

Bacteria capable of utilizing citrate make an enzyme called citrate ______ or _____

Back 12

permease
citrase

Front 13

The _____ test utilizes Simmon's ____ media to determine if a bacterium can utilize citrate as its sole carbon and energy source.

Back 13

citrate
citrate

Front 14

The reagent of the Indole test is _____ reagent. ____ color

Back 14

Kovac's
pink

Front 15

Bacteria capable of utilizing citrate make an enzyme called citrate ______ or _____

Back 15

permease
citrase

Front 16

The reagent of the MR test is _______ _______

Back 16

methylene red

Front 17

The reagent of the Indole test is _____ reagent. ____ color

Back 17

Kovac's
pink

Front 18

The reagent of the MR test is _______ _______

Back 18

methylene red

Front 19

The reagent of the VP test is Barritt's test _______

Back 19

A and B

Front 20

The reagent of the VP test is Barritt's test _______

Back 20

A and B

Front 21

The media in Simmon's media contains a ph indicator increases above ____bromthymol ____. Slant is blue rest ____

Back 21

7.4
blue
green

Front 22

The endproducts of Methyl Red are ____ The endproducts of Voges-Proskauer are ____ or acetoin, _______, and ethyl alcohol.

Back 22

acid
nonacid.
2,3 butanediol

Front 23

The reagent in Citrate test is pH indicator _____ blue with range of 6-7.6

Back 23

bromthymol

Front 24

The endproducts of the citrate test are sodium ______

Back 24

bicarbonate

Front 25

Uninoculated Simmon's citrate agar contains has a ph of 6.9 and it green. When alkaline turns ____

Back 25

blue

Front 26

An auxotroph is a microorganism that lacks the ability to +++++ one or more essential _____ factors . May arise by the occurence of one or more +++++ in a prototroph.

Back 26

synthesze
growth
mutation

Front 27

The form of gene transfer and recombination in bacteria that requries direct cell contact is+++++++

Back 27

conjugation

Front 28

A plasmid that carries genes for bacterial conjugation and makes its E coli host cell the gene donor during conjugation

Back 28

F Factor

Front 29

A bacterium that donates its genes with high _____ to a recipient cell during conjugation by integrating the ____ Factor into the bacterial chromosome is a ______.

Back 29

frenquency
F
Hfr bacterium

Front 30

A _____ is a circular double stranded DNA molecule that can exist and _____ independantly of the +++++ or may be integrated with it. A plasmid is stably _____ but is not required for the host cell's growth and reproduction.

Back 30

replicate
chromosome
inherited

Front 31

Plate 1 Fnegative E Coli autxotrophic, thr-, leu-, thi-, and str-r. Although the bacterium is streptomycin ++++, it does not have the genes to synthesize essential amino acids threonine and ++++ and thimine. Streptomyocin ______

Back 31

lucine
resistant

Front 32

The second culture is Hfr or +++++ frequency recombinat male. The male is prototropic ____ type and has genes to synthesize amino acids threonine, leucine, and thiatine. It is streptomyocin +++++

Back 32

high
wild
resistant

Front 33

Plate 1 Fnegative E Coli autxotrophic, thr-, leu-, thi-, and str-r. Although the bacterium is streptomycin ++++, it does not have the genes to synthesize essential amino acids threonine and ++++ and thimine. Streptomyocin ______

Back 33

lucine
resistant

Front 34

Mixture Culture Growth should occur. F bacterium is +++++ resistant, but unable to synthesize +++++ and leucine. After incubation, F- will get threonine and leucine genes from ____ bacterium.

Back 34

threonine
Hfr

Front 35

The second culture is Hfr or +++++ frequency recombinat male. The male is prototropic ____ type and has genes to synthesize amino acids threonine, leucine, and thiatine. It is streptomyocin +++++

Back 35

high
wild
sensitve

Front 36

Mixture Culture Growth should occur. F bacterium is +++++ resistant, but unable to synthesize +++++ and leucine. After incubation, F- will get threonine and leucine genes from ____ bacterium.

Back 36

threonine
Hfr

Front 37

Transformation or genetic ++++ allows for genetic +++++ in binary fission bacteria.

Back 37

transfer
variation

Front 38

In Transformation, Conjugation, and Transduction all three involve a ____ and ++++. The donor DNA is subsequently integrated in +++++ into the genome of the recipient's DNA. If the donor contains different genes, the recipient assumes donor's traits or ++++++

Back 38

donor
recipient
phenotype

Front 39

The _____ ____test name anoncronym is Indole

Back 39

Tryptone Broth

Front 40

The Methyl Red acronym is ______ test.

Back 40

MR-VP broth

Front 41

The Volger-Proshauer acronym is _____ _____.

Back 41

MR=VP broth

Front 42

The Citrate is the acronym for ++++++ ______ agar.

Back 42

Simmon's Citrate

Front 43

IMViC differentiates Klebsiella, ++++++, and ++++++.

Back 43

Enterobacter
E Coli

Front 44

E Coli produces large quantities of +++++ from the metabolism of ______. Not further _____, stable acids.

Back 44

acid,
glucose
metabolized

Front 45

Klebsiella and ________ produce acid from _____ metabolism but further metabolize the acid to produce ______ end products

Back 45

Entobacter
glucose
neutral

Front 46

The acronym for the _____ _____ Agar test is Citrate.

Back 46

Simmon's Citrate

Front 47

Barritt B is _____ hydroxide for Vi or ______ ______ or MR-VP.

Back 47

potassium
Voges Proskauer

Front 48

the sole source of carbon in Simmon's Citrus Agar is +++++ _____ media.

Back 48

Simmon's citrus

Front 49

The enzyme is citrase or citrate _______

Back 49

permease

Front 50

Barritt's A reagent contains +++++ ______

Back 50

alpha-naphthol

Front 51

The chemical in Barrit's B are _____ _______

Back 51

Potassium Hydroxide

Front 52

MR/VP broth contains _____ and peptone.

Back 52

glucose

Front 53

An ______ is a microorganism which lacks the ability to synthesize one or more essential ______ factors. Arises from the occurrence of ______ in a _______

Back 53

auxotroph
growth
mutation
prototroph

Front 54

A ______ is a microorganism that requires the same _____ as the _____ of naturally occurring members of its species.

Back 54

prototroph
nutrients
majority

Front 55

Conjugation is the form of gene ____ and _____ in bacteria that requires cell to cell contact.

Back 55

transfer
recombination

Front 56

A combinations of genes or chromosomes that differ from the ______ generation is _____

Back 56

parent's
recombination

Front 57

The _____ is the bacterial cell that naked DNA is transferred from. The cell that receives naked DNA is the ______

Back 57

donor
recipient

Front 58

The physical traits of the donor are the _____

Back 58

phenotye

Front 59

Bacterial genetic exchange promotes bacterial _____ and adaption.

Back 59

variety

Front 60

The plasmid was transformed as shown by the _____ plate's bacterial +++++

Back 60

mixture
growth

Front 61

The F Factor is a _____ that carries genes with high frequency to a _____ cell during conjugation by integrating the F Factor into the bacterial ______

Back 61

fertility
recipient cell
chromosome\

Front 62

A _____ is a circular double stranded DNA molecule that can exist and ____ independently of the chromosome or may be integrated with it. Stably _____ but not required for the host cell's growth and ______

Back 62

plasmid
replicate
heritable
reproduction