Micro Lab 1

Front 1

Are free living microorganisms pathogenic or non-pathogenic?

Back 1

Non-Pathogenic

Front 2

Saprophyes perform

Back 2

decomposition

Front 3

If microorganisms cause a disease they are

Back 3

pathogenic

Front 4

May benefit but have no significant impact on host

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commensal microbes

Front 5

Are capable of producing disease if introduced to suitable part of body

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Opportunistic pathogens

Front 6

Area where microbe resides and serves as potential source of infection

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Reservoir

Front 7

A medium that contains living microbes

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culture

Front 8

If culture contains single species, it is a

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pure culture

Front 9

It is important to transfer microbes from pure culture to sterile medium, ___, without contamination.

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aseptically

Front 10

Used to grow microbes when fresh or large number of cells are required.

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Broths

Front 11

used to grow stock cultures, refridgerated after incubation and maintained for several weeks

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agar slants

Front 12

used for obtaining isolation of species, differential testing, quantifying bacterial densities

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plated media

Front 13

used to sterilize wire loops/needles

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bacteriological incinerator

Front 14

Microbial culture of 2 or more species

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mixed culture

Front 15

Commonly used isolation technique

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streak plate

Front 16

During streaking, cell density __, leading to individual cells being deposited separately on agar surface

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decreases

Front 17

formed when isolated cells grow, consisting of only original cell type

Back 17

colonies

Front 18

some colonies form pairs, chains, clusters, called

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colony forming unit (CFU)

Front 19

method of isolation which a diluted microbial sample is deposited on agar plate and spread uniformly across the surface with a glass rod.

Back 19

spread plate

Front 20

what is the purpose of streak plate method?

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after incubation, part of an isolated colony can be transferred to a sterile medium to begin a pure culture.

Front 21

a visible mass of cells that appears where original cell was deposited

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colony

Front 22

basic shape categories

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round, irregular, punctiform

Front 23

basic margin categories

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entire, smooth, undulate, lobate, filamented (unbranched), rhizoid (branched)

Front 24

basic surface categories

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smooth, rigid, wrinkled

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basic texture categories

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moist, mucoid (sticky), butryous (buttery), dry, shiny, dull

Front 26

basic elevation categories

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flat, raised, convex, pulvinate (very convex), umbonate

Front 27

uses transmitted light and magnifying glass to allow for observation of greater detail; colony color and other features are best seen with reflected light

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colony counter

Front 28

most organisms produce __ growth, which is dense and opaque with a smooth edge

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filiform

Front 29

__ is a crusty growth

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Friable

Front 30

2 important factors that influence bacterial growth are

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incubation time, temperature

Front 31

In a broth, an organism that floats on top of the medium and produces a type of surface membrane

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pellicle

Front 32

uniform fine turbidity is

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cloudy

Front 33

bacteria that appear to clump is

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flocculent growth

Front 34

___ show charateristic growth patterns in broth that make it easier to identify an organism.

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bacterial genera