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34 Cards in this Set

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What type of media is Blood Agar?
differential
What type of media is Chocolate Agar?
neither, it's the same as blood agar, except the blood is preheated to allow nutrients like hemin and NAD to escape from erythrocytes.
What type of stuff is the Chocolate Agar for?
bacteria w/ very specific nutritional needs, such as H. influenzae
What type of media is MacConkey Agar?
selective/ differential
What kind of stuff grows on MacConkey Agar?
ONLY gram negatives
Bacteria that utilize lactose on MacConkey Agar produce what color colonies?
pink
Bacteria that are unable to utilize lactose as a sugar source grow what color colonies on the MacConkey Agar?
clear to brownish
MacConkey Agar is abbreviated:
MAC
Chocolate Agar is abbreviated:
CHOC
Blood Agar is abbreviated:
BAP
When deciding the appearance of the colonies, you should look for:
color,
size,
shape,
elevation
Other things to observe about colonies/plate include:
odors,
hemolysis patterns,
discolorations
Normal microbial flora are most prevalent where:
oral cavity,
upper respiratory tract,
the colon,
the skin,
the lower genitourinary tract
What body areas are normally free from microbes:
blood,
cerebrospinal fluid,
deep tissues
Those bacteria other than normal flora in/on the body are called:
strict pathogens
The appearance of the bacterial colonies on a plate is called:
colony morphology
Gram stain that binds to peptidoglycan:
Crystal Violet
permanently attaches CV to PPG
Iodine
removes the CV from the gram negative bacteria, leaving them "invisible"
alcohol
the red dye used to stain the gram negatives
safranin
Gram positives stain
purple
Gram negatives stain
pink
The control stain is made up of
S. aureus and E. coli
S. aureus is
G+
E. coli is
G-
The first step to prepare a gram stain is to get the slide ready by:
Making 2 nickel sized circles on the slide, and labeling one end. Then, flame the inoculating loop to red hot, and briefly allow to cool. Place a very small drop of fresh tap water in a circle, and flame the loop again. Touch the colony gently with the loop and mix with water. Spread the mixture to the edges, and fan w/ hand or paper to allow the smear to air dry. Pick up the slide with a clothespin b/w the circles, and run through the flame 3-4 times. Allow slide to cool briefly.
The next step is staining with:
CRYSTAL VIOLET. wait 15 to 30 seconds, then gently rinse with water under the faucet.
Then, drip ________ solution onto each circle and wait __________.
IODINE: 15 to 30 seconds, rinse gently with water.
Add ________ dropwise until the purple dye ___________.
95% ETHANOL: running, then immediately rinse with water
Drip ___________onto each circle and wait _________.
SAFRANIN: 15 to 30 seconds, gently rinse, blot dry on fresh bibulous paper
These bacteria are ALWAYS catalase +
staphylococci
These bacteria are ALWAYS catalase -
streptococci
The catalase test uses:
hydrogen peroxide (H202)
A positive catalase test is indicated by :
bubbles!