Micro Exam I

Front 1

Catalase

Back 1

Positive: Staph
Negative: Strep

Presence of bubbles indicates positive result

Front 2

Coagulase

Back 2

Positive: Staph. aureus
Negative: Coag. neg. Staph

Clotting of rabbit plasma indicates positive result

Front 3

Bacitracin disk

Back 3

Used for beta hemolysis Strep and to distinguish Micrococcus from Staph

Reistant: Group B Strep (Strep. agalactiae)

Staph

Susceptible: Group A Strep (Strep. pyogenes)

Micrococcus

Front 4

Optichin disk

Back 4

Used for alpha hemolysis Strep

Resistant: viridans Strep.

Susceptible: Strep. pneumoniae

Front 5

Bile Esculin

Back 5

Streak on slant agar. Positive reaction is agar turns black at least 3/4 of the way down the tube. Used with 6.5% NaCl

Enterococcus: positive
viridans Strep: negative
Group D Strep: positive

Front 6

6.5% NaCl

Back 6

Place organism in broth. Positive result is indicated by growth, secondary indication is color change from purple to yellow

Enterococcus: positive
viridans Strep: negative
Group D Strep: negative

Front 7

Furazolidone

Back 7

Used to detect Staph. vs. Micrococcus

Resistant: Micrococcus

Susceptible: Staph

Front 8

Novobiocin

Back 8

Only used with urine samples. Used to detect Staph. saprophyticus vs. other coag. neg. Staph

Resistant: Staph. saprophyticus

Susceptible: Coag. neg. staph (Staph. epidermidis)

Front 9

CAMP

Back 9

Used to determine Group A vs. Group B Strep. Positive result is indicated by presence of zone of hemolysis in the shape of an arrowhead

Positive: Group B
Negative: Group A

Front 10

Why stab SB agar when streak?

Back 10

To look for production of Streptolysin O. It is only produced in reduced state.

Front 11

Why check coagulase agar after 4 hours?

Back 11

Some Staph. spp produce fibrolysin, which will dissolve the clot and produce a false negative if checked after 24 hours

Front 12

Coag. positive Staph other than S. aureus

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Staph. intermedius
Staph. hyicus
Staph. delphini

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Biohazard clean up steps

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1- absorb
2- bleach
3- wait
4- dispose in biohazard bag/sharps container

Front 14

bacterial vs. viral meningitis

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bacterial: glucose levels decrease while protein levels increase and neutrophil count goes up

viral: glucose and protein stay at normal levels while lymph count goes up

Front 15

Meningitis in newborns caused by

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Lysteria
Group D Strep

Front 16

Meningitis in young kids caused by

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H. influenzae
if vaccinated, Strep. pneumoneae

Front 17

Meningitis in young adults caused by

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Neisseria meningitidis

Front 18

Which body fluid is empyema found in

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pleural fluid

Front 19

What organisms are found in peritoneal fluid?

Back 19

anaerobes and Enterobacter

Front 20

What organisms are found in synovial fluid?

Back 20

Staph. aureus
Neisseria gonorhoea

Front 21

What two organisms are vanvomyocin resistant?

Back 21

Leuconostoc and Pediococcus

Front 22

Cause of impetigo

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Strep. pyogenes

Front 23

Cause of gas gangrene

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Clostridium perfringens

Front 24

Cause of cutaneous abcess

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Staph. aureus

Front 25

Cause of infection in dog and cat bites

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Pasturella multocida

Front 26

Salient features of E. coli

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LF, flat and dry colonies on MAC
Large, gray, moist colonies on SB

indole +
citrate -

Front 27

Salient features of Klebsiella

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LF, mucoid colonies on MAC


pneumonieae: lys + orn -
aerogenes: lys + orn +
cloacea: lys - orn +

Front 28

Salient features of Proteus

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NLF, swarms and stinks

mirabilis: indole - orn +
vulgaris: indole + orn -

Front 29

Even I find every freaking, crappy, nasty, muscle.

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Eryspelas
Impetigo
Folliculitis
Ecthyma
Furunculosis
Cellulitis
Necrotizing fasciitis
Myonecrosis

Front 30

Cause of meningitits in elderly

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E. coli
Strep. pneumoniae
Listeria

Front 31

Signs and symptoms of meningitis

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fever
headache
lethargy
vomiting
stiff neck

Front 32

What are the main causes of pleural empyema?

Back 32

Anaaerobes
S. aureus
gram negative bacilli

Front 33

Cause of septic arthritis

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Staph. aureus

Front 34

Cause of infections of prosthetic joints

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Staph. epidermidis
coag. neg. staph

Front 35

Enteric genus that is a plant pathogen

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Erwinia

Front 36

Yellow pigmented enteric

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sakazakii

Front 37

Escherichia species other than E. coli

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hermanii

Front 38

Escherichia species that is pigmented

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hermanii

Front 39

Enterobacter species other than cloacae or aerogenes

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sakazakii

Front 40

Serratia species other than marcescens

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rubidaea