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32 Cards in this Set

  • Front
  • Back

Antisepsis

Destruction of pathogenic organisms on living objects

Disinfection

killing of microbes on non-living surfaces, kills vegetative cells but not endospores

Sterile

100% microbe free

Best way to prevent transmission

hand washing

Chemical control methods

detergents


phenols


heavy metal


halogens


aldehides



Physical control methods

heat


pasteurization


autoclave/moist pressure


Dry heat


Freezing


refridgeration


salting


radiation



thermal death point

lowest temp to kill microbes in 10 min

thermal death time

shortest time to kill all test microbes at specified temp

Organisms destroyed by antibiotics

Bacteria (prokaryotes)



Variables on microbe control

time of exposure


concentration


organic materal


toxicity


amount of microbes


type of microbe (endospores, cysts, viruses, prions)


temp & pH of environment

how microbes are attacked

Cell wall


Denaturing proteins


DNA


Plasma membrane



types of heavy metals used for control

zinc


copper


silver


mercury



halogens used in control

flourine


chlorine


Bromine


Iodine



How refridgeration and freezing work to control microbes

slow down enzymes

how radiation works in control

damages DNA

how heavy metals work in control

denature proteins

injury heavy metals will be used for

burns

how hydrogen peroxide works

oxygen ions damage proteins and DNA in anaerobes

liquid aldehydes

Glutaraldehyde


Formaldehyde

antibiotics that inhibit cell walls

penicillins


cephalosporins


vancomhycin


bacitracin


isoniazid

antibiotics that inhibit cell membrane

polymyxins (surface only)

Antibiotics that inhibit replication of DNA

Quinolones (ciprofloxacin)



What antibiotics that inhibit replication of DNA actually inhibit

gyrase (unwinding enzyme)

Antibiotics that inhibit protein synthesis in prokaryotes

chloramphenicol


erthromycin

Antibiotics that inhibit protein synthesis in eukaryotes

gentamicin


streptomycin


tetracyclines

antibiotics that inhibit enzymes and products

sulfonamides (sulfa drugs)


trimethoprim

Antibiotic that inhibits RNA polymerase

Rifampin

Side effects of antibiotics

Allergies


Diarrhea


Fungal infections of mouth (destruction of normal flora)

Antibiotic used as last resort

Vancomycin

causes of antibiotic resistence

incorrect prescription


Patient incompetence


animal feed

solutions to resistence

microbe testing


2 drug cocktails


washing food

hospital microbe levels of control

handwashing


Gloves and gown


mask


low pressure rooms with filtration