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65 Cards in this Set

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CHEMICALLY DEFINED MEDIUM
A CULTURE MEDIUM IN WHICH THE EXACT CHEMICAL COMPOSITION IS KNOWN
THERMOPHILE
AN ORGANISM WHOSE OPTIMUM GROWTH TEMPERATURE IS BETWEEN 50 AND 60 DEGREES C; A HEAT LOVING MICROBE
PSYCHROPHILE
AN ORGANISM THAT GROWS BEST AT ABOUT 15 DEGREES C AND DOES NOT GROW ABOVE 20 DEGREES C; A COLD-LOVING MICROBE
HYPERTHERMOPHILE
AN ORGANISM WHOSE OPTIMUM GROWTH TEMPERATURE IS AT LEAST 80 DEGREES C; ALSO CALLED EXTREME THERMOPHILE
REDUCING MEDIUM
A CULTURE MEDIUM CONTAINING INGREDIENTS THAT WILL REMOVE DISSOLVED OXYGEN FROM THE MEDIUM TO ALLOW THE GROWTH OF ANAEROBES
EXTREME HALOPHILE
AN ORGANISM THAT REQUIRES A HIGH SALT CONCENTRATION FOR GROWTH
HYDROXYL RADICAL
A TOXIC FORM OF OXYGEN (OH*) FORMED IN CYTOPLASM BY IONIZING RADIATION AND AEROBIC RESPIRATION
MOST PROBABLE NUMBER (MPN) METHOD
A STATISTICAL DETERMINATION OF THE NUMBER OF COLIFORMS PER 100ml OF WATER OR 100g OF FOOD
DIRECT MICROSCOPIC COUNT
ENUMERATION OF CELLS BY OBSERVATION THROUGH A MICROSCOPE
ENRICHMENT CULTURE
A CULTURE MEDIUM USED FOR PRELIMINARY ISOLATION THAT FAVORS THE GROWTH OF A PARTICULAR MICROORGANISM
OBLIGATE HALOPHILE
AN ORGANISM THAT REQUIRES HIGH OSMOTIC PRESSURES SUCH AS HIGH CONCENTRATIONS OF NaCl
LAG PHASE
THE TIME INTERVAL IN A BACTERIAL GROWTH CURVE DURING WHICH THERE IS NO GROWTH
NUTRIENT BROTH
A COMPLEX MEDIUM MADE OF BEEF EXTRACT AND PEPTONE
OPTIMUM GROWTH TEMPERATURE
THE TEMPERATURE AT WHICH A SPECIES GROWS BEST
OBLIGATE ANAEROBE
AN ORGANISM THAT DOES NOT USE MOLECULAR OXYGEN (O2) AND IS KILLED IN THE PRESENCE OF 02
FILTRATION
THE PASSAGE OF A LIQUID OR GAS THROUGH A SCREENLIKE MATERIAL; A 0.45um FILTER REMOVES MOST BACTERIA
POUR PLATE METHOD
A METHOD OF INOCULATING A SOLID NUTRIENT MEDIUM BY MIXING BACTERIA IN THE MELTED MEDIUM AND POURING THE MEDIUM INTO A PETRI DISH TO SOLIDIFY
BACTERIAL GROWTH CURVE
A GRAPH INDICATING THE GROWTH OF A BACTERIAL POPULATION OVER TIME
BUDDING
(1) ASEXUAL REPRODUCTION BEGINNING AS A PROTUBERANCE FROM THE PARENT CELL THAT GROWS TO BECOME A DAUGHTER CELL (2) RELEASE OF AN ENVELOPED VIRUS THROUGH THE PLASMA MEMBRANE OF AN ANIMAL CELL
MESOPHILE
AN ORGANISM THAT GROWS BETWEEN ABOUT 10 DEGREES C AND 50 DEGREES C; A MODERATE-TEMPERATURE-LOVING MICROBE
AGAR
A COMPLEX POLYSACCHARIDE DERIVED FROM A MARINE ALGA AND USED AS A SOLIDIFYING AGENT IN CULTURE MEDIA
STREAK PLATE METHOD
A METHOD OF ISOLATING A CULTURE BY SPREADING MICROORGANISMS OVER THE SURFACE OF A SOLID CULTURE MEDIUM
PLASMOLYSIS
LOSS OF WATER FROM A CELL IN A HYPERTONIC ENVIRONMENT
FACULTATIVE ANAEROBE
AN ORGANISM THAT CAN GROW WITH OR WITHOUT MOLECULAR OXYGEN (O2)
DEATH PHASE
THE PERIOD OF LOGARITHMIC DECREASE IN A BACTERIAL POPULATION; ALSO CALLED LOGARITHMIC DECLINE PHASE
DIFFERENTIAL MEDIUM
A SOLID CULTURE MEDIUM THAT MAKES IT EASIER TO DISTINGUISH COLONIES OF THE DESIRED ORGANISM
STERILE
FREE OF MICROORGANISMS
SELECTIVE MEDIUM
A CULTURE MEDIUM DESIGNED TO SUPPRESS THE GROWTH OF UNWANTED MICROORGANISMS AND ENCOURAGE THE GROWTH OF DESIRED ONES
PEROXIDASE
AN ENZYME THAT BREAKS DOWN HYDROGEN PEROXIDE
COLONY
A VISIBLE MASS OF MICROBIAL CELLS ARISING FROM ONE CELL OR FROM A GROUP OF THE SAME MICROBES
COMPLEX MEDIUM
A CULTURE MEDIUM IN WHICH THE EXACT CHEMICAL COMPOSITION IS NOT KNOWN
BINARY FISSION
PROKARYOTIC CELL REPRODUCTION BY DIVISION INTO TWO DAUGHTER CELLS
INOCULUM
A CULTURE MEDIUM IN WHICH MICROORGANISMS ARE IMPLANTED
ACIDOPHILE
A BACTERIUM THAT GROWS BELOW pH 4
PEROXIDE ANION
AN OXYGEN ANION CONSISTING OF TWO ATOMS OF OXYGEN
PLATE COUNT
A METHOD OF DETERMINING THE NUMBER OF BACTERIA IN A SAMPLE BY COUNTING THE NUMBER OF COLONY-FORMING UNITS ON A SOLID CULTURE MEDIUM
SUPEROXIDE DISMUTASE (SOD)
AN ENZYME THAT DESTROYS SUPEROXIDE FREE RADICALS
SUPEROXIDE FREE RADICAL
A TOXIC FORM OF OXYGEN (O2*) FORMED DURING AEROBIC RESPIRATION
TRACE ELEMENT
A CHEMICAL ELEMENT REQUIRED IN SMALL AMOUNTS FOR GROWTH
LYOPHILIZATION
FREEZING A SUBSTANCE AND SUBLIMATING THE ICE IN A VACUUM; ALSO CALLED FREEZE-DRYING
CATALASE
AN ENZYME THAT CATALYZES THE BREAKDOWN OF HYDROGEN PEROXIDE TO WATER AND OXYGEN
MINIMUM BACTERICIDAL CONCENTRATION (MBC)
THE LOWEST CONCENTRATION OF CHEMOTHERAPEUTIC AGENT THAT WILL KILL TEST MICROORGANISMS
NUTRIENT AGAR
NUTRIENT BROTH CONTAINING AGAR
PSCYHROTROPH
AN ORGANISM THAT IS CAPABLE OF GROWTH BETWEEN ABOUT 0 DEGREES C AND 30 DEGREES C
SPREAD PLATE METHOD
A PLATE COUNT METHOD IN WHICH INOCULUM IS SPREAD OVER THE SURFACE OF A SOLID CULTURE MEDIUM
AEROTOLERANT ANAEROBE
AN ORGANISM THAT DOES NOT USE MOLECULAR OXYGEN (O2) BUT IS NOT AFFECTED BY ITS PRESENCE
LOG PHASE
THE PERIOD OF BACTERIAL GROWTH OR LOGARITHMIC INCREASE IN CELL NUMBERS; ALSO CALLED EXPONENTIAL GROWTH PHASE
STATIONARY PHASE
THE PERIOD IN A BACTERIAL GROWTH CURVE WHEN THE NUMBER OF CELLS DIVIDING EQUALS THE NUMBER DYING
ORGANIC GROWTH FACTOR
AN ESSENTIAL ORGANIC COMPOUND THAT AN ORGANISM IS UNABLE TO SYNTHESIZE
MICROAEROPHILE
AN ORGANISM THAT GROWS BEST IN AN ENVIRONMENT WITH LESS MOLECULAR OXYGEN (O2) THAN IS NORMALLY FOUND IN AIR
FACULTATIVE HALOPHILE
AN ORGANISM CAPABLE OF GROWTH IN, BUT NOT REQUIRING, 1-2% SALT
SERIAL DILUTION
THE PROCESS OF DILUTING A SAMPLE SEVERAL TIMES
GENERATION TIME
THE TIME REQUIRED FOR A CELL OR POPULATION TO DOUBLE IN NUMBER
MINIMAL INHIBITORY CONCENTRATION (MIC)
THE LOWEST CONCENTRATION OF A CHEMOTHERAPEUTIC AGENT THAT WILL PREVENT GROWTH OF THE TEST MICROORGANISMS
EXTREMOPHILE
A MICROORGANISM THAT LIVES IN ENVIRONMENTAL EXTREMES OF TEMPERATURE, ACIDITY, ALKALINITY, SALINITY, OR PRESSURE
MINIMUM GROWTH TEMPERATURE
THE LOWEST TEMPERATURE AT WHICH A SPECIES WILL GROW
DNA POLYMERASE
ENZYME THAT SYNTHESIZES DNA BY COPYING A DNA TEMPLATE
CULTURE
MICROORGANISMS THAT GROW AND MULTIPLY IN A CONTAINER OF CULTURE MEDIUM
SINGLET OXYGEN
HIGHLY REACTIVE MOLECULAR OXYGEN (O2)
DEEP-FREEZING
PRESERVATION OF BACTERIAL CULTURES AT -50 TO -95 DEGREES C
OBLIGATE AEROBE
AN ORGANISM THAT REQUIRES MOLECULAR OXYGEN (O2) TO LIVE
CAPNOPHILE
A MICROORGANISM THAT GROWS BEST AT RELATIVELY HIGH CO2 CONCENTRATIONS
MAXIMUM GROWTH TEMPERATURE
THE HIGHEST TEMPERATURE AT WHICH A SPECIES CAN GROW
NITROGEN FIXATION
THE CONVERSION OF NITROGEN (N2) INTO AMMONIA
TURBIDITY
THE CLOUDINESS OF A SUSPENSION