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62 Cards in this Set

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prokaryote
a unicellular organism having cells lacking membrane-bound nuclei
Prime examples of prokaryote?
bacteria
blue-green algae
eukaryote
singled-called or multicellular organism whose cells contain a distinct membrane-bound nucleus.
bacteriology
the study of bacteria
mycology
study of fungi
virology
study of viruses
parasitology
the study of protozoa and parasitic worms
serotype
group of microorganisms, viruses, or cells classified together based on their cell surface antigens.
anitgen
a molecule that binds specifically to an antibody
What are microbes?
free living organism so small (less than 100 micrometers). Only visible under microscope.

include bacteria, protozoa, microscopic algae, nonliving viruses.

most are necessary and crucial. they are everywhere.
Nomenclature

each organism has 2 names which are
1. genus, capitalized
2. species (specific epithet), not capitalized

*both underlined and italicized
*once mention once, cam abbreviate initial of the genus
Who made the naming system used today?
Carolus Linnaeus (1735)
How microbes are named and 2 examples
describe organism, honor researcher, identity the habitat of a species
*Saphylococcus aureus describes shape and arrangement, and the golden color of the colonies
*Escherichia coli named for scientist and reminds us it lives in colon.
3 major groups of Phylogenetic tree of life
BACTERIA
ARCHAEA
EUCARYOTA
most microbes are _____________
PROKARYOTES
What are examples of microbes classified as eukaryotes?
*algae and protozoa (protists)
*some fungi
*multicellular animal parasites microscopic during some stages of life
Types of prokaryote microbes
*bacteria
*archaea- oldest on planet. don't cause disease
Are viruses technically microbes?
no, they are not living unless in a host cell
Bacteria
*unicellular
*peptidoglycan cell walls
*reproduce by bianary fission
*very diverse. For energy use organic or inorganic chemicals, photosynthesis. (they're everywhere because they eat everything!)
*many can swim using flagella
Archaea
(prokaryote)
*unicellular
*lack peptidoglycan cell walls
*have eukaryotic-like ribosomes
*live in extreme environment
*include methanogens, extreme halophiles, extreme thermophiles
Fungi (Eukaryote)
*chitin cell walls
*organic chemicals for energy (decomposers. no photosynthesis)
*molds and mushrooms are MULTIcellular- masses of mycelia composed of branching hyphae filaments
*yeast are UNIcellular
Protozoa (Eukaryotes)
*unicellular, complex cells
*absorb or ingest organic chemicals
*may be motile, via pseudopods (false feet, extension of cytoplasm), cilia, or flagella
*everything that aren't algae or fungi, various shapes and sizes
Algae (eukaryotes)
*callulose walls
*photosynthesis for energy
*produce oxygen and organic compounds
*multicellular and unicellular
Cyrptosporidiosis
*Cryptosporidium protozoa
*first reported in 1976
*causes 30% of diarrheal illness in developing countries
*in US transmitted via water (water treatment center has to watch)
Viruses
*acellular (aren't cells)
*consist of DNA or RNA core
*core is surrounded by a protein coat
*coat may be enclosed in a lipid envelope
*replicate only when in living cell
multicellular animal parasites
*microscopic stages in life cycles
*parasitic flatworms and roundworms are called HELMINTHS
*multicellular animals
*not strictly microorganisms, but have medical importance
see table 12.1
do it!
Naming Classes
Domain
Phylum
Class
Order
Family
Genus
Species
Robert Hooke
1665 observed living thing were composed of little boxes --> beginning of cell theory! looked at cork. observed eukaryotes.(funig, mold, yeast)
Anton van Leeuwenhoek
dutch merchant first to actually see miroorganisms. 1676, discovers bacteria with higher resolution microscope. made detailed drawings of "animalcules". becteria and protezoa
Ibn Khatima
14th century
hypothesized that infectious diseases are caused by "minute bodies" which enter the human body and cause disease
Black Death
(Ibn Khatima)
killed 30-60% of Europe's population
spontaneous generation
hypothesis that living organisms arrive from nonliving matter; "a vital force" forms life. Need air. animals could be born of soil, etc
Francesco Redi
1668
wanted to disprove spontaneous generation.
Jars with meat experiment. Jar with mesh over didn't produce maggots. Serious blow to s.g. Still didn't completely disprove spontaneous generation.
biogenesis
hypothesis that the living organisms arise from living organisms.

battle with religion- faith favored spontaneous generation
John Needham
1745 put boiled broth into covered flasks. nutriend broth boiled, then placed in sealed flask grew microbes.
Supported S.G!
Lazzaro Spallanzani
1765- boiled broth in sealed flasks. no growth. disproved S.G.! Except people didn't believe because "vital force" (air) wasn't available for growth.
Rudolf Virchow
1858 said cells arise from preexisting cells. brings back biogenesis! CELL THEORY- all living things are composed of cells and come from preexisting cells.
Louis Pasteur
1822-1895 finally proves SG wrong! Uses flasks with S-bent necks. First concluded microbes were in the air.
showed microbes can be present in nonliving matter, that they can be destroyed by heat, and that you can block them. beggining of aseptic techniques!!
The Golden Age of Microbiology
1857 to 1914. Spearheaded by Pasteur and Robert Koch
Fermentation and Pasteurization
Pasteur demonstrated that spoilage bacteria could be killed by heat

fermentation- yeast converts sugar to alcohol in absence of air.

Pasteurization is the application if high heat for a short time. reduces spoilage
Germ Theory of Disease
Possibility that microorganisms caused disease. Hard for people to accept, they thought disease was punishment for misdeed.
Agostino Bassi
1835- showed that a fungus silkworm disease was caused by a protozoan
Pasteur
1865- believed that another silkworm disease was caused by a protozoan. Found way to ID afflicted silkworm moths.
Ignaz Semmelweis
1840s- advocated hand washing to prevent transmission of puerperal fever from one OB patient to another
Joseph Lister
1860s- applied germ theory to medical procedures. Treated surgical wounds with phenol solution (kill bacteria)
Robert Koch
1876- proved that bacterium causes anthrax and provided the experimental steps (Koch's Postulates) to prove that a specific microbe causes a specific disease.
Koch's Postulates
(4 Steps)
1. The microorganism found in diseased animals, not in healthy
2. The microorganism is isolated from diseased animals and grows in pure culture
3. The cultured microorganism causes diseases if injected into healthy host
4. The new microorganism isolated is identical to the original organism
Problems with Koch's Postulates
*sometimes can't isolate microbe
*need to isolate right microbe
*not everyone gets infected same way
*not always ethical
Edward Jenner
1796- inoculated a person with cowpox virus, who was protected from smallpox
Vaccination is derived from term vacca, for cow
Protection called immunity
Immunology
the study of immunity. Vaccines and interferons are being investigated to prevent and cure viral diseases.
Chemotherapy
treatment of disease by using chemicals.
antibiotics are chemicals naturally produced by bacteria and fungi to act against another organism.
synthetic drugs are produced in laboratory.
Paul Ehrlich
1910 synthesized an arsenic agent he used to treat syphilis.
Alexander Fleming
1928- discovered first antibiotic! Penicillin! Accidentally.
Penecillin
discovered that Penicillium fungus made an antibiotic that killed S. aureus.
tested and mass produced in 1940s
1. Bacteriology
2. Mycology
3. Parasitology
1. study of bacteria
2. study of fungi
3. study of protozoa and parasitic worms
genomics
study of all of an organism's genes. Has allowed csientists to classify bacteria based and fungi according to their genetic relationships with other bacteria, fungi, and protozoa. New tools for classifying organisms.
microbial genetics
study of how microbes inherit traits
molecular biology
the study of how DNA directs protein synthesis
recombinant DNA
DNA made from two different sources.
Paul Berg in 1960s inserted animal DNA into bacterial DNA and produced animal protein.
normal microbiota
we have a variety of microbes on and inside our body. Do no harm, or benefit.
biofilm
complex aggregation of microbes. attached to eachother and/or some solid surface.