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49 Cards in this Set

  • Front
  • Back
The purpose of the Catalase test
To identify if organism makes enzyme catalase.
Catalase test
Indicator/ Reagent
Hydrogen-peroxide

Medium for Catalase test
None

Catalase test results
positive: bubbles

negative: no bubbles
Nitrate Reduction test purpose
To show microbe reduce Nitrate to Nitrite, Nitrogen gas , Ammonia, or no reduction.
Nitrate Reduction Test
Indicator/ Reagent
Nitrate regents A and B if no nitrogen gas.
Zinc powder if no reduction.
Nitrate Reduction test
Medium
Nitrate broth with Durham tube
Nitrate Reduction test results
Positive: with Nitrate A and B :Red
Negative: No change
Positive with Zinc powder: colorless
Negative with zinc powder: Red
IMViC test
Indole test
MR test
VP test
Citrate test
IMViC test Media
3 media: SIM deep, MRVP broth, Simmon citrate agar slant w/ Ph indicator
Indole test purpose
to test for motility, H2S and Indole production
Indole test Indicator/ Reagent
Kovac's reagent
Indole test Medium
SIM deep
Indole test results
Motility: + diffusion - no diffusion
H2S: + black and - not black
Indole: Red ring on top - not red ring on top
Methyl-Red test ( MR) Purpose
determine the use of glucose and Acid
MR test Indicator/ Reagent
Methyl-Red ( 8 drops)
MR test Media
MRVP broth
MR test results
Positive(Acidic) : Red- glucose user
Negative (Basic): Yellow- Not glucose user
Voges-Proskauer test Purpose
determine the production of Acetoin
V-P test Indicator/ Reagent
Barritt's A (12 drops)
Barritt's B ( 4 drops)
V-P test Media
MRVP broth
V-P test Results
Positive: Red- Acetoin
Negative: Yellow
Oxydase test Purpose
to show microbe can use oxygen (oxydase)
Oxydase test Indicator/ Reagent
( tetranethyl-p- phenylenediamine)
TMPD
Medium of Oxydase test
Filter paper
Oxydase test results
Positive: Purple
Negative: Colorless
Simmon's Citrate Test Purpose
Identify the use of citrate as sole Carbon source
Citrate Test

Indicator/ Reagent
BROMOTHYMOL- BLUE
(ph indicator)
Citrate Test Media
Simmon's citrate agar slant w/ ph indicator
Citrate Test Results
Positive : Royal-blue color
Negative: green
Decarboxylation Test Purpose
To see bacteria can decarboxitate and produce the decarboxylase enzyme
Decarboxylation Test

Indicator/ Reagent
Bromcresol Purple
Decarboxylation Test Media
3 Media: with decarboxylase broth (arginine, lysine, ornithine)
Decarboxylation Test Results
Positive: Purple (basic)
Negative: Yellow (Acidic)
Deamination Test Purpose
To see if organism able to deaminate(remove) Phenylalanine
Deamination Test Indicator/Reagent
Ferric-Chlorine (10-15 drops)
(FeCl 3)
Deamination Test Medium
Phenylalanine agar slant
Deamination Test Results
Positive: Avocado green (dominase)
Negative: not avocado green
Urea Test Purpose
To determine if microbe can break down Urea
Urea Test Indicator/ Reagent
Phenol-Red as ph indicator
Urea Test Media
Urea broth w/ph indicator
Urea Test Results
Positive: Hot pink ( Urease)
Negative: Yellow
Gelatin Hydrolysis Purpose
to see the ability of the microbe to break down protein gelatin (gelatinase)
Gelatin Hydrolysis
Indicator/ Reagent
None
Gelatin Hydrolysis Media
Gelatin deep
Gelatin Hydrolysis test Results
Positive: Liquid gelatinase
Negative: Solid gelatinase
Bacteria that can grow on air
Obligate aerobe
facultative anaerobe, and microaerophile
Bacteria that will grow in gas pack jar
Obligate anaerobe, Facultative anaerobe
Bacteria that can grow in candle jar
Obligate anaerobe, Facultative anaerobe, microaerophile