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15 Cards in this Set

  • Front
  • Back
Homologous Recombination
Involves the physical exchange of genetic material between homologous DNA
Plasmid mediated transfer of DNA. There is a donor and a recipeint strain. Normally the plasmid is transferred; however, in some cases chromosomal DNA can be transferred.
Self transmissible plasmid
Encode contact functions and readies its DNA for transfer.
Donor cell with integrated plasmid
F plasmid can integrate into the chromosome by homologous recombination, and subsequently transfer chromosomal DNA to a recipient cell.
Readies its DNA for transfer (cuts at oriT)
encodes contact functions (tra genes) F also has a vegetative origin of replication, oriV.
What genes transfer first in Hfr?
Genes adjacent to oriT
How are genes mapped?
By minutes
What is the "grandfather" plasmid
What size are most self transmissible plasmids?
56-107 kb
When selecting for mutatants you must always select against what strain?
The donor strain
What is the phenotype class for the Ti plasmid?
What is the phenoype for TetR,StrR,CmR ect..?
antibiotic resistance
What is the phenotype for colicin E2, E3 ect or subtilisin?
Bacteriocin resistance (and prodution)
RecA protein
Coats DNA, finds appropriate region on chromosome.