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15 Cards in this Set
- Front
- Back
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Homologous Recombination
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Involves the physical exchange of genetic material between homologous DNA
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Conjugation
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Plasmid mediated transfer of DNA. There is a donor and a recipeint strain. Normally the plasmid is transferred; however, in some cases chromosomal DNA can be transferred.
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Self transmissible plasmid
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Encode contact functions and readies its DNA for transfer.
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Donor cell with integrated plasmid
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F plasmid can integrate into the chromosome by homologous recombination, and subsequently transfer chromosomal DNA to a recipient cell.
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Mobilizable
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Readies its DNA for transfer (cuts at oriT)
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Conjugative
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encodes contact functions (tra genes) F also has a vegetative origin of replication, oriV.
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What genes transfer first in Hfr?
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Genes adjacent to oriT
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How are genes mapped?
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By minutes
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What is the "grandfather" plasmid
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pBR322
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What size are most self transmissible plasmids?
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56-107 kb
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When selecting for mutatants you must always select against what strain?
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The donor strain
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What is the phenotype class for the Ti plasmid?
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Virulence
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What is the phenoype for TetR,StrR,CmR ect..?
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antibiotic resistance
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What is the phenotype for colicin E2, E3 ect or subtilisin?
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Bacteriocin resistance (and prodution)
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RecA protein
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Coats DNA, finds appropriate region on chromosome.
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