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15 Cards in this Set

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6 Steps in General Tissue Prep for Microscopy:
1. Tissue Fixation
2. Dehydration
3. Clearing
4. Staining
5. Sectioning
6. Embedding
What are 4 chemicals used for Tissue Fixation?
-Formaldehyde
-Glutaraladehyde
-Alcohol
-Osmium
How are tissues Dehydrated for microscopy?
By gradually removing water by replacing it with alcohol.
What is achieved by "Clearing" tissues for microscopy prep?
The alcohol is replaced with a solvent miscible for embedment.
What is Embedding tissue?
Placing it in a hard medium like plastic or paraffin.
What is used to Section tissues for microscopy?
A Microtome
Purpose of Staining tissues:
To improve the contrast of cells.
What are the 2 main types of dyes?
-Acidic --> Eosin

-Basic --> Hematoxylin
What does Eosin stain?
Eosin is an acidic dye with extra electrons - therefore it is itself negatively charged, and stains positive molecules.
What does Hematoxylin stain?
Hematoxylin is a basic, positively charged dye; it stains negatively charged molecules that donate electrons.
What terms are used to denote macromolecules stained by Eosin and Hematoxylin?
Eosin: stains Acidophilic

Hematoxylin: stains Basophilic
What cell components are Basophilic?
Nucleic acid - it's an acid, so it loves base.
What cell component is Acidophilic?
Cytoplasm
What is Schiff Reagent used for?
Staining DNA or glycogen
What is Sudan dye used for?
Staining accumulated lipids