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15 Cards in this Set

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  • Back
6 Steps in General Tissue Prep for Microscopy:
1. Tissue Fixation
2. Dehydration
3. Clearing
4. Staining
5. Sectioning
6. Embedding
What are 4 chemicals used for Tissue Fixation?
How are tissues Dehydrated for microscopy?
By gradually removing water by replacing it with alcohol.
What is achieved by "Clearing" tissues for microscopy prep?
The alcohol is replaced with a solvent miscible for embedment.
What is Embedding tissue?
Placing it in a hard medium like plastic or paraffin.
What is used to Section tissues for microscopy?
A Microtome
Purpose of Staining tissues:
To improve the contrast of cells.
What are the 2 main types of dyes?
-Acidic --> Eosin

-Basic --> Hematoxylin
What does Eosin stain?
Eosin is an acidic dye with extra electrons - therefore it is itself negatively charged, and stains positive molecules.
What does Hematoxylin stain?
Hematoxylin is a basic, positively charged dye; it stains negatively charged molecules that donate electrons.
What terms are used to denote macromolecules stained by Eosin and Hematoxylin?
Eosin: stains Acidophilic

Hematoxylin: stains Basophilic
What cell components are Basophilic?
Nucleic acid - it's an acid, so it loves base.
What cell component is Acidophilic?
What is Schiff Reagent used for?
Staining DNA or glycogen
What is Sudan dye used for?
Staining accumulated lipids