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15 Cards in this Set
- Front
- Back
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6 Steps in General Tissue Prep for Microscopy:
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1. Tissue Fixation
2. Dehydration 3. Clearing 4. Staining 5. Sectioning 6. Embedding |
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What are 4 chemicals used for Tissue Fixation?
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-Formaldehyde
-Glutaraladehyde -Alcohol -Osmium |
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How are tissues Dehydrated for microscopy?
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By gradually removing water by replacing it with alcohol.
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What is achieved by "Clearing" tissues for microscopy prep?
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The alcohol is replaced with a solvent miscible for embedment.
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What is Embedding tissue?
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Placing it in a hard medium like plastic or paraffin.
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What is used to Section tissues for microscopy?
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A Microtome
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Purpose of Staining tissues:
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To improve the contrast of cells.
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What are the 2 main types of dyes?
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-Acidic --> Eosin
-Basic --> Hematoxylin |
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What does Eosin stain?
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Eosin is an acidic dye with extra electrons - therefore it is itself negatively charged, and stains positive molecules.
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What does Hematoxylin stain?
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Hematoxylin is a basic, positively charged dye; it stains negatively charged molecules that donate electrons.
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What terms are used to denote macromolecules stained by Eosin and Hematoxylin?
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Eosin: stains Acidophilic
Hematoxylin: stains Basophilic |
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What cell components are Basophilic?
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Nucleic acid - it's an acid, so it loves base.
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What cell component is Acidophilic?
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Cytoplasm
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What is Schiff Reagent used for?
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Staining DNA or glycogen
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What is Sudan dye used for?
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Staining accumulated lipids
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