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Learning objective

What are the different stages of maturation seen in the granulocytes?

Stages of maturation seen in granulocytes


List

Hematopoietic Stem Cell


CMP


GMP


Myeloblast


Promyelocyte


Myelocyte


Metamyelocyte


Band


Segmented (polymorphonuclear) cell


Stages of maturation seen in granulocytes



Stage 1

Hematopoietic stem cell

Stages of maturation seen in granulocytes



Stage 1: _________ stem cell

hematopoietic stem cell

Stages of maturation seen in granulocytes



Stage 1: ___________ stem __________

hematopoietic


cell

Stages of maturation



Stage 1: Hematopoietic ____ cell

Stem

Stages of maturation



Stage 1

Hematopoietic Stem cell

Stages of maturation seen in granulocytes



Stage 2

CMP

Stages of maturation seen in granulocytes



Stage 1


Stage 2: _MP

Hematopoietic stem cell


C

Stages of maturation seen in granulocytes



Stage 1


Stage: C__

Hematopoietic stem cell


MP

Stages of maturation seen in granulocytes



Stage 2

CMP

Stages of maturation seen in granulocytes



Stage 1


Stage 2

Hematopoietic stem cell


CMP

Stages of maturation seen in granulocytes



Stage 3

GMP

Stages of maturation seen in granulocytes



Stage 1


Stage 2


Stage 3: _MP

Hematopoietic Stem Cell


CMP


G

Stages of maturation seen in granulocytes



Stage 1


Stage 2


Stage 3: G ____

Hematopoietic Stem Cell


CMP


MP

Stages of maturation seen in granulocytes



Stage 3

GMP

Stages of maturation seen in granulocytes



Stage 1-3

Hematopoietic Stem Cell


CMP


GMP

Stages of maturation seen in granulocytes



Stage 4

Myeloblast

Stages of maturation seen in granulocytes



Stage 1


Stage 2


Stage 3


Stage 4: Myelo_______

Hematopoietic Stem Cell


CMP


GMP


blast

Stages of maturation seen in granulocytes



Stage 4: ______blast

Myelo

Stages of maturation seen in granulocytes



Stage 4

Myeloblast

Stages of maturation seen in granulocytes



Stage 1-4

Hematopoietic Stem Cell


GMP


CMP


Myeloblast

Stages of maturation seen in granulocytes



Stage 5

Promyelocyte

Stages of maturation seen in granulocytes



Stage 1-4


Stage 5: Promyelo_____

Hematopoietic Stem Cell


GMP


CMP


Myeloblast



cyte

Stages of maturation seen in granulocytes



Stage 5: Pro_____cyte

myelo


Stages of maturation seen in granulocytes



Stage 1-4


Stage 5: ___myelo____

Hematopoietic stem


CMP


GMP


Myeloblast



Pro


cyte

Stages of maturation seen in granulocytes



Stage 5: Pro_________

myelocyte

Stages of maturation seen in granulocytes



Stage 5

Promyelocyte

Stages of maturation seen in granulocytes



Stage 1-5

Hematopoietic Stem Cell


CMP


GMP


Myeloblast


Promyelocyte

Stages of maturation seen in granulocytes



Stage 6

Myelocyte

Stages of maturation seen in granulocytes



Stage 1-4


Stage 5: promyelo___


Stage 5: myelo_____

Hematopoietic Stem cell


GMP


CMP


Myeloblast



cyte


cyte

Stages of maturation seen in granulocytes



Stage 4: ______blast


Stage 5: pro_____cyte


Stage 6: _____cyte

myelo


myelo


myelo

Stages of maturation seen in granulocytes



Stage 4: ______blast


Stage 5: pro__________


Stage 6: ___________

myelo


myelocyte


myelocyte

Stages of maturation seen in granulocytes



Stage 6

Myelocyte

Stages of maturation seen in granulocytes



Stage 1-6

Hematopoietic Stem Cell


CMP


GMP


Myeloblast


Promyelocyte


Myelocyte

Stages of maturation seen in granulocytes



Stage 7

Metamyelocyte

Stages of maturation seen in granulocytes



Stage 1-3


Stage 4: _____blast


Stage 5: pro______


Stage 6: ___________


Stage 7: meta______________

Hematopoietic Stem Cell


GMP


CMP



Myelo


Myelocyte


myelocyte


myelocyte

Stages of maturation seen in granulocytes



Stage 4: myelo____


Stage 5: _____myelocyte


Stage 6: Myelocyte


Stage 7: M___myelocyte

blast


pro


eta

Stages of maturation seen in granulocytes



Stage 4: myelo____


Stage 5: _____myelocyte


Stage 6: Myelocyte


Stage 7: _____myelocyte

blast


pro


meta

Stages of maturation seen in granulocytes



Stage 7: _____myelocyte

meta

Stages of maturation seen in granulocytes



Stage 7: Meta_________

myelocyte

Stages of maturation seen in granulocytes



Stage 7

Metamyelocyte

Stages of maturation seen in granulocytes



Stage 1-7

Hematopoietic stem cell


GMP


CMP


Myeloblast


Promyelocyte


Myelocyte


Metamyelocyte

Stages of maturation seen in granulocytes



Stage 8

Band

Stages of maturation seen in granulocytes



Stage 1-7


Stage 8: Ba__

Hematopoietic Stem Cell


GMP


CMP


Myeloblast


Promyelocyte


Myelocyte


Metamyelocyte


band

Stages of maturation seen in granulocytes



Stage 8: B____

band

Stages of maturation seen in granulocytes



Stage 8

Band

Stages of maturation seen in granulocytes



1-8

Hematopoietic Stem Cell


GMP


CMP


Myeloblast


Promyelocyte


Myelocyte


Metamyelocyte


Band

Stages of maturation seen in granulocytes



Stage 9

Segmented (polymorphonuclear) cell

Stages of maturation seen in granulocytes



Stage 1-8


Stage 9: SEGMENTED (POLYmorphoNUCLEAR) ____

Hematopoietic Stem Cell


GMP


CMP


Myeloblast


Promyelocyte


Myelocyte


Metamyelocyte


Band


cell

Stages of maturation seen in granulocytes



Stage 9: ___________ (_____MORPHO____) ____

segmented


poly


nuclear


cell

Stages of maturation seen in granulocytes



Stage 8


Stage 9: Segmented (Poly_____nuclear) _____

band


morpho


cell

Stages of maturation seen in granulocytes



Stage 7


Stage 8


Stage 9: ___________ (polymorpho_____) cell

Metamyelocyte


Band


Segmented


nuclear


Cell

Stages of maturation seen in granulocytes



Segmented cell


Stage?


aka?

Stage 9


Polymorphonuclear

Stages of maturation seen in granulocytes



Stage 9

Segmented (polymorphonuclear) cell

Stages of maturation seen in granulocytes



Stage 1-9

Hematopoietic Stem Cell


GMP


CMP


Myeloblast


Promyelocyte


Myelocyte


Metamyelocyte


Band


Segmented (polymorphonuclear) cell

Stages of maturation seen in granulocytes



Trend: size

Increasingly smaller



Stage 4 and 5: 10-20 microns


Stage 6 and 7: 10-18 microns


Stage 8 and 9: 10-16 microns

Stages of maturation seen in granulocytes



Trend: size



Increasingly _______


Stage 4 and 5: 10-20 _________


Stage 6 and 7: 10-18 __________


Stage 8 and 9: 10-16 __________


smaller


microns


microns


microns

Stages of maturation seen in granulocytes



Trend: size



________ smaller


Stage _ and _: 10-20 ________


Stage _ and _: 10-18 _______


Stage _ and _: 10-16 ________

increasingly


4


5


microns


6


7


microns


8


9


microns

Stages of maturation seen in granulocytes



Trend: size



_____________


Stage 4 and 5: ___-20 microns


Stage 6 and 7: __-18 microns


Stage 8 and 9: __-16 microns

Increasingly


10


10


10

Stages of maturation seen in granulocytes



Trend: size



________________


Stage 4 and 5: 10-20 microns


Stage 6 and 7: 10-___ microns


Stage 8 and 9: 10-___ microns



-2

increasingly small


18


16

Stages of maturation seen in granulocytes



Trend: size



____________


Stage 4 and 5: 10-__ microns


Stage 6 and 7: 10-___ microns


Stage 8 and 9: 10-___ microns

increasingly smaller



20


18


16

Stages of maturation seen in granulocytes



Trend: size



________________


Stage 4 and 5: ________________


Stage 6 and 7: ______________


Stage 8 and 9: ________________

increasingly smaller


10-20


10-18


10-16 microns

Stages of maturation seen in granulocytes



Trend: size

Increasingly smaller



Stage 4 and 5: 10-20 microns


Stage 6 and 7: 10-18 microns


Stage 8 and 9: 10-16 microns

Stages of maturation seen in granulocytes



Trend: nucleus

Stage 4 and 5: round; 4: light reddish purple, 5 reddish purple



Stage 6: Oval or round



Stage 7: kidney or barrel shaped, horseshoe-shaped



Stage 8: narrow band of uniform thickness



Stage 9: 2-5 lobes




Stages of maturation seen in granulocytes



Trend: nucleus



Stage 4 and 5: _______; 4: light reddish purple, 5 _____________



Stage 6: Oval or _________



Stage 7: KIDNEY or barrel ____________



Stage 8: NARROW band of uniform ________



Stage 9: 2-5 _____

round


reddish purple



round



shaped



thickness



lobes

Stages of maturation seen in granulocytes


Trend: nucleus



Stage 4 and 5: _______; 4: light ___________, 5: _______________



Stage 6: Oval or ___________



Stage 7: ____________ or barrel shaped



Stage 8: _______ band of uniform ________



Stage 9: _-5 _________

round


reddish purple


reddish purple



round



kidney



narrow


thickness



2


lobes



Stages of maturation seen in granulocytes


Trend: nucleus



Stage 4 and 5: ______ ; 4: ______reddish purple, 5 reddish purple



Stage 6: _________ or round



Stage 7: kidney or _______ shaped



Stage 8: narrow _______ of _______ thickness, horseshoe-______



Stage 9: 2-__ _______

round


light


oval


barrel


band


uniformed


horseshoe



5


lobes


Stages of maturation seen in granulocytes


Trend: nucleus



Stage 4 and 5: _______; 4: _____, 5:___________



Stage 6: ________ or _______



Stage 7: _________ or ______ _________



Stage 8: ____________ of _____________, _____-_____



Stage 9: ______________

round


light reddish purple


reddish purple



oval


round



kidney or barrel shaped



narrow band


uniformed thickness


Horseshoe-shaped



2-5 lobes


Stages of maturation seen in granulocytes


Trend: nucleus

Stage 4 and 5: round; 4: light reddish purple, 5 reddish purple



Stage 6: Oval or or round



Stage 7: kidney or barrel shaped



Stage 8: narrow band of uniform thickness, horseshoe-shaped



Stage 9: 2-5 lobes

Stages of maturation seen in granulocytes


Trend: chromatin pattern

Stage 4: fine


Stage 5: slightly clumped


Stage 6: More clumped


Stage 7: Clumping is easily distinguished


Stage 8: clumped


Stage 9: clumped

Stages of maturation seen in granulocytes


Trend: chromatin pattern



Stage 4: ____


Stage 5: slightly _______


Stage 6: More _________


Stage 7: Clumping is easily dis______


Stage 8: ________


Stage 9: __________

fine


clumped


clumped


distinguishable


clumped


clumped

Stages of maturation seen in granulocytes


Trend: chromatin pattern



Stage 4: _________


Stage 5: _______ clumped


Stage 6: ______ clumped


Stage 7: _________ is easily distinguished


Stage 8: ___________


Stage 9: _________

fine


slightly


more


clumping


clumped


clumped

Stages of maturation seen in granulocytes


Trend: chromatin pattern



Stage 4: _______


Stage 5: _____ clumped


Stage 6: _______ clumped


Stage 7: Clumping is _______ distinguished


Stage 8: clumped


Stage 9: clumped

fine


slighly


more


easily

Stages of maturation seen in granulocytes


Trend: chromatin pattern

Stage 4: fine


Stage 5: slightly clumped


Stage 6: More clumped


Stage 7: Clumping is easily distinguished


Stage 8: clumped


Stage 9: clumped

Stages of maturation seen in granulocytes



Trend: Nucleoli

Stage 4: 1-3


Stage 5: 0-2


Stage 6: 0-1


Stage 7-9: lost


Stages of maturation seen in granulocytes



Trend: Nucleoli



Stage 4: 1-3


Stage 5: _-2


Stage 6: _-1


Stage 7-9: lost

0

Stages of maturation seen in granulocytes



Trend: Nucleoli



Stage 4: 1-_


Stage 5: 0-_


Stage 6: 0-_


Stage 7-9: __

3


2


1


lost

Stages of maturation seen in granulocytes



Trend: Nucleoli



Stage 4: _-3


Stage 5: _-2


Stage 6: _-1


Stage 7-9: _________

1


0


0


Lost

Stages of maturation seen in granulocytes



Trend: Nucleoli

Stage 4: 1-3


Stage 5: 0-2


Stage 6: 0-1


Stage 7-9: lost

Stages of maturation seen in granulocytes



Acidity of the cytoplasm

Stage 4 is deeply basophilic



Increasingly less basophilic

Stages of maturation seen in granulocytes



Acidity of the cytoplasm



Stage 4 is deeply _____


Increasingly less __________

basophilic


basophilic

Stages of maturation seen in granulocytes



Acidity of the cytoplasm



_________ is deeply _________


_________ less __________

stage 4


basophilic


increasingly


basophilic

Stages of maturation seen in granulocytes



Acidity of the cytoplasm



Stage 4 is _______________


Increasingly _____________

deeply basophilic


less basophilic

Stages of maturation seen in granulocytes



Acidity of the cytoplasm

Stage 4 is deeply basophilic


Increasingly less basophilic

Stages of maturation seen in granulocytes



Trend: Color of the cytoplasm

From purple in stage 4


to


Blue-tan/pink in stage 6


to


Tan/Pink in stage 7-9

Stages of maturation seen in granulocytes



Trend: Color of the cytoplasm



From ________ in stage 4


to


______-tan/pink in stage 6


to


_____/_____ in stage 7-9

Purple


Blue


tan/pink

Stages of maturation seen in granulocytes



Trend: Color of the cytoplasm



From ________ in stage 4


to


______________ in stage 6


to


____________ in stage 7-9

purple


Blue -tan pink


Tan/pink

Stages of maturation seen in granulocytes



Trend: Color of the cytoplasm



From purple in _________


to


Blue-tan/pink in _________


to


Tan/Pink in _________

stage 4


Stage 6


Stage 7-9

Stages of maturation seen in granulocytes



Trend: Color of the cytoplasm

From purple in stage 4


to


Blue-tan/pink in stage 6


to


Tan/Pink in stage 7-9

Stages of maturation seen in granulocytes



Trend: granulation

Increasingly specific



Stage 4: N


Stage 5: fine azurophilic, nonspecific granules


Stage 6: Both specific and nonspecific granules


Stage 7-9: specific granules

Stages of maturation seen in granulocytes



Trend: granulation



Increasingly ___________



Stage 4: __


Stage 5: fine azurophilic, non_______ granules


Stage 6: Both ___________ and non_________ granules


Stage 7-9: ______ granules

specific


N


specific


specific


specific


specific

Trend: granulation



__________ specific



Stage 4: ___


Stage 5: ______ azurophilic, ___________ granules


Stage 6: Both _________ and _________ granules


Stage 7-9: __________ granules

increasingly


N


fine


nonspecific


specific and nonspecific


specific

Trend: granulation



Trend: _________________



Stage 4: _


Stage 5: fine ______________, ________________


Stage 6: Both ________________________


Stage 7-9: _______________

Increasingly specific


N


azurophilic


nonspecific


specific and nonspecific grnaules


Specific granules

Trend: granulation

Increasingly specific



Stage 4: N


Stage 5: fine azurophilic, nonspecific granules


Stage 6: Both specific and nonspecific granules


Stage 7-9: specific granules

Stages in the maturation of granulocytes



Trend: size of cytoplasm

Increasingly more abundant (moderate)

Stages in the maturation of granulocytes



Trend: size of cytoplasm


Increasingly more ________ (moderate)

abundant

Stages in the maturation of granulocytes



Trend: size of cytoplasm


________________ abundant (_________)

increasingly more


abundant

Stages in the maturation of granulocytes



Trend: size of cytoplasm

Increasingly more abundant (moderate)