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8 Cards in this Set

  • Front
  • Back
Gram staining
Used to make bacteria in infectious specimens more visible.
Differential stain that distinguishes cells with a gram-positive
cell wall from those with a gram-negative cell wall
Gram-positive bacteria
- stains purple
-Staphylococcus, Streptococcus, Bacillus, Clostridium, Micrococcus
Gram-negative bacteria
- stains pink
-E. Coli, Salmonella, Neisseria
Gram-staining stain colors
Primary stain = Crystal violet (purple)
Counter stain = Safranin (pink)
Acid-fast stain colors
Primary stain = Carbolfuschia (fuschia)
Counterstain = Methylene blue
Acid fast stain purpose
- acid-fast bact vs. non
- to detect Mycobacterium tuberculosis; leprosy
- acid-fast = pink vs. non = blue
Endospore stain colors
Primary stain = Malachite green = Endospores
Counter stain = Safranin = Pink (rest of cell)
Important gram + spore-forming bacteria
Bacillus
Clostridium