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8 Cards in this Set
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Gram staining
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Used to make bacteria in infectious specimens more visible.
Differential stain that distinguishes cells with a gram-positive cell wall from those with a gram-negative cell wall |
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Gram-positive bacteria
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- stains purple
-Staphylococcus, Streptococcus, Bacillus, Clostridium, Micrococcus |
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Gram-negative bacteria
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- stains pink
-E. Coli, Salmonella, Neisseria |
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Gram-staining stain colors
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Primary stain = Crystal violet (purple)
Counter stain = Safranin (pink) |
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Acid-fast stain colors
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Primary stain = Carbolfuschia (fuschia)
Counterstain = Methylene blue |
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Acid fast stain purpose
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- acid-fast bact vs. non
- to detect Mycobacterium tuberculosis; leprosy - acid-fast = pink vs. non = blue |
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Endospore stain colors
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Primary stain = Malachite green = Endospores
Counter stain = Safranin = Pink (rest of cell) |
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Important gram + spore-forming bacteria
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Bacillus
Clostridium |