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15 Cards in this Set

  • Front
  • Back
What are the three components of DNA?
1. sugar (deoxyribose)
2. an acid
3. organic "bases": ATCG
What are three properties of the nucletoide bases?
1. weakly basic in solution
2. hydrophobic and aromatic
3. planar molecules that show resonance
What does resonance do to the bases?
1. bases can interact with each other when stacked by van der Waal forces
2. Bases absorb strongly in UV light
What are the similarties of DNA and RNA?

What are the difference?
1. Long polymers
2. nucleotide base
3. sugar residuce
4. phophodiester back bone

1. bases
2. sugar group
What are 5 traits of the WC DNA?
1. sugar phosphate back bone is a right-handed helx
2. basese are internal
3. complementary bases
4. anti-parallel
5. the two sugar-phophate backbones are not exactly opposite to each other (major and minor grooves)
What are three forms of DNA and how do their helices turn?
A = right
B = right
Z = left
Are Z DNA postively or negatively supercoiled?
What factors stabilze DNA structure?
1. H bond of bp
2. Hydrophobic effects
3. stacked bases form van der Waals
4. DNA backbone interacts with cations
Which is more stable RNA or DNA, why?
DNA because RNA has 2' and 3' carbons that can form phosphodiester bonds and DNA only has one which makes it more stable at a higher pH
Why does DNA contrain thymine rather than uracil?
B/C C will deaminate to U spontaneously.
What is required for the biosynthesis of T?
Folic acid from vegetables
What happens in the absence of T?
It will block excision-repair
1. mutagensis
2. chromosome breaks
3. brith defects
4. aging of cells
5. induction of cancer
What are three ways we can denature DNA?
1. Heat
2. high pH (OH-)
3. Urea

all these things destroy H bonds of bases
What is the hyperhcromic shift?
denatured DNA abosrobs more UV light (260nm) than native DNA. THis is due to the lack of base stacking
Why does DNA renaturation require annealing at intermediate temp. or pH?
1. quick cooling gives inter-strand interactions
2. Snap back regions of DNA self complementation gives intra-stand interactions