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12 Cards in this Set
- Front
- Back
Recombinant DNA |
DNA sequences that never existed together in nature |
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Necessities for recombinant DNA |
1) restriction endonuclease 2) molecular palindrome |
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Restriction endonuclease |
Cuts in the middle of the DNA |
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Molecular palindromes |
Reads the same 5 Prime to 3 Prime on each strand |
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Sticky ends |
Single stranded outhangings produced by restriction endonuclease |
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Vector |
DNA molecule that acts as a shuttle for DNA of interest and allows DNA of interest to be replicated in cells |
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Plasmids |
Bacterial circular DNA molecules |
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Gene cloning |
Making duplicates of a piece of DNA |
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Polymerase chain reaction |
Technique used to amplify specific sequence of DNA Needs DNA polymerase |
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Denaturation |
Heat up DNA to separate into single strands |
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Anneling |
Add primers to form base Paris with temple dna |
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Restriction mapping |
Determining where on a strand of DNA we find restriction endonucleases sites |