• Shuffle
    Toggle On
    Toggle Off
  • Alphabetize
    Toggle On
    Toggle Off
  • Front First
    Toggle On
    Toggle Off
  • Both Sides
    Toggle On
    Toggle Off
  • Read
    Toggle On
    Toggle Off
Reading...
Front

Card Range To Study

through

image

Play button

image

Play button

image

Progress

1/12

Click to flip

Use LEFT and RIGHT arrow keys to navigate between flashcards;

Use UP and DOWN arrow keys to flip the card;

H to show hint;

A reads text to speech;

12 Cards in this Set

  • Front
  • Back

Recombinant DNA

DNA sequences that never existed together in nature

Necessities for recombinant DNA

1) restriction endonuclease


2) molecular palindrome

Restriction endonuclease

Cuts in the middle of the DNA

Molecular palindromes

Reads the same 5 Prime to 3 Prime on each strand

Sticky ends

Single stranded outhangings produced by restriction endonuclease

Vector

DNA molecule that acts as a shuttle for DNA of interest and allows DNA of interest to be replicated in cells

Plasmids

Bacterial circular DNA molecules

Gene cloning

Making duplicates of a piece of DNA

Polymerase chain reaction

Technique used to amplify specific sequence of DNA


Needs DNA polymerase

Denaturation

Heat up DNA to separate into single strands

Anneling

Add primers to form base Paris with temple dna

Restriction mapping

Determining where on a strand of DNA we find restriction endonucleases sites