M2-C3-L80 --> Dna Tumor Virology

Front 1

List the human DNA tumor viruses of the:

- polyomaviruses x3
- herpesviruses x2
- hepadnoviruses x1

Back 1

BK virus
JC virus
SV-40 virus

EBV
KSHV

Hepatitis B virus

Front 2

List the human RNA tumor viruses of the:

- retroviruses x1
- flavivirus x1

Back 2

HTLV-1

Hepatitius C virus

Front 3

Cellular oncogenes are deregulated by what? x2

Back 3

Overexpression

Mutation

Front 4

Viral oncogenes defined.

Back 4

Virus-acquired cellular proto-oncogenes.

Front 5

Loss of function for what gene leads to CA?

Back 5

Tumor suppressor gene

Front 6

Effects on cellular genes:

- RNA tumor viruses
- DNA tumor viruses

Back 6

Activates proto-oncogenes

Negates cellular TUMOR SUPPRESSOR GENE

Front 7

What is the primary function of proto-oncogenes?

Back 7

Signal transduction from cell surface to the nucleus.

(also has growth stimulation potential)

Front 8

DNA tumor viruses target what two pathway altering proteins?

Back 8

Rb (Retinoblastoma protein)

p53

Front 9

DNA vs RNA tumor viruses oncogenes:

- v-onc enssential?
- cellular homologue?
- affects signaling pathway how?
- association with strategy for viral replication

Back 9

DNA: essential viral genes
RNA: NOT essential viral genes

DNA: NO cellular homologue
(v-onc not cellularly obtained)
RNA: acquired cellular genes

DNA: INACTIVATE tumor suppressors
RNA: DEREGULATE signal pathway

DNA: linked
RNA: unrelated

Front 10

What type of target cell does DNA tumor virus like to undergo transformation?

Back 10

Quiescent
Differentiated
Non-conducive to viral replication

Front 11

Viral oncogenes encoded by ______ preparing the cell for?

Back 11

Early genes

Viral replication

Front 12

DNA tumor virus transformation will inhibit what 2 tumor suppressor proteins that prevent what progression?

Back 12

Rb and p53

G1 progression

Front 13

List the three small DNA tumor viruses.

Back 13

HPV

SV40

Adenovirus

Front 14

For DNA tumor viruses, transformation only occurs when?

what happens here?

Back 14

in the "aborted" viral life cycle

Early genes expressed
Replication (which is cytocidal) does not occur

Front 15

Adenovirus:

- gene product
- cellular target

Back 15

E1A -> Rb
E1B -> p53

Front 16

SV 40:

- gene product
- cellular target

Back 16

Large T antigen -> Rb

Front 17

Polyomavirus

- gene product
- cellular target

Back 17

Large T antigen -> Rb

Middle T antigen -> Src and PI3K

Front 18

Papillomavirus

- gene product
- cellular target

Back 18

E7 -> Rb
E6 -> p53
E5 -> PDGR Receptors

Front 19

What would you seen in CIN I for a HPV infection? x4

Back 19

Differentiated Cells
Infectious virus
Koilocytes
Episomes

Front 20

What would you seen in CIN III for a HPV infections? x4

Back 20

Undifferentiated Cells AT ALL LAYERS
Integrated virus DNA
Cancer
High Dysplasia

Front 21

For HPV, an E2 disruption upon integration would lead to what event?

Back 21

Loss of regulation for E6 and E7

Front 22

During HPV integration what oncogenes are preserved? x3

Back 22

E6
E7
LCR (long control region)

Front 23

During HPV integration what oncogenes are lost? x2

Back 23

E2

Other genes needed to replicate virus are lost

Front 24

What are the outcomes of small DNA tumor virus infection? x3

Back 24

Cell cycle deregulation
(no consequence if cell dies secondary replicative infection)

Monoclonal tumors
(same viral DNA integration site in all cells)

Transformation (rare, but possible in non-permissible cells)

Front 25

Transformation requires what? x2

Back 25

Abortive infection

Integration of intact viral gene

Front 26

EBV LMP1 mimics what?

E5 gene of bovine papillomavirus mimics what?

Back 26

CD40 receptor

PDGF Receptor

Front 27

HHV-8:

- # of strands in genome
- linear or circular genome
- what subfamily?

Back 27

double stranded

Linear, but circular once inside cell

Gammaherpesvirus

Front 28

HHV-8 (or KSHV)

- Ubiquitous?
- Strong risk factor?
- #1 Reservoir

Back 28

NOT ubiquitous
(very uncommon)

AIDS

B-cells

Front 29

Kaposi's sarcoma:

- clinical symptoms
- driven by what process

Back 29

Multiple vascular nodules of skin, mucous membrane, and viscera

Cytokine

Front 30

Kaposi's sarcoma:

- What would you expect to see on a histological slide? x4

Back 30

Proliferation of Spindle/Endothelial cells

Infiltrating inflammatory cells

Extravasation of erythrocytes

Extensive Neoangiogenesis

Front 31

Kaposi's sarcoma:

- transmission x2
- endemic where?
- epidemic where?
- classic KS?

Back 31

sexual
saliva

Central Africa
(younger age, more women, aggressive)

AIDS patients (homos)

Older Mediterranean men
(benign, distal extremities)

Front 32

Detection of HHV-8 in all histological views show what forms?

What does this indicate?

Back 32

Identical

Common etiology

Front 33

T/F - Kaposi's is only longitudinal transmission.

Back 33

False.

Can be vertical also.

Front 34

What tests would detect HHV8 and get samples from where? x4

Back 34

PCR

saliva
semen
prostate
peripheral blood lymphs

Front 35

HHV8 "pirates" cellular genes that regulate what? x3

Back 35

Angiogenesis

Cell cycle

Inflammation

Front 36

List three diseases associated with HHV8.

Back 36

Kaposi's Sarcoma

Body cavity-based primary effusion lymphomas

Multicentric Castlemen's Disease

Front 37

Body cavity-based primary effusion lymphomas:

- describe mass
- what molecules are not expressed
- often co-infected with what other virus?

Back 37

Non-contiguous mass

Adhesion molecules

EBV

Front 38

Multicentric Castlemen's Disease

- describe mass
- location
- what cellular process involved?

Back 38

Non-neoplastic lymphoproliferation

Generalized or as solitary mediastinal node

IL-6 overexpression (viral or cellular)

Front 39

viral IL-6 expression is relevant in the pathogenesis of what two diseases?

Back 39

Kaposi's and Castlemans

Front 40

List the HHV-8 pirated cellular proto-oncogenes and describe what they do? x5

Back 40

1. viral Bcl-2 (APOPTOSIS regulator)

2. viral IL-6 (ANTI-APOPTOSIS, B-Cell growth stimulator)

3. viral G-Protein coupled receptor (ANGIOGENIC activity)

4. viral IFN regulatory factor (negative regulator of INF signaling)

5. viral Cyclin (Cell Cycle regulator)

Front 41

Which are the lytic cellular proto-oncogenes that are pirated by HHV-8?

Back 41

Bcl-2

IL-6

G-protein-coupled receptor

Front 42

KS is the most common malignancy associated with what other disease?

Back 42

AIDS

Front 43

What has dropped the incidence of KS by 50-75%?

Back 43

HAART

Front 44

T/F - KSHV Genome encodes genes that are homologous to cell-signaling and regulatory pathway proteins.

If this were true, what is this called?

Back 44

TRUE

Molecular mimicry

Front 45

KSHV is a DNA tumor virus with oncogenes acquired from what genes?

Back 45

Cellular