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33 Cards in this Set

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Where does glycolysis take place?
Cytosol
Where does Pentose Phosphate Pathway take place?
Cytosol
Where does TCA occur?
Matrix of mitochondria
Where does Pyruvate Dehydrogenase occur?
Matrix of mitochondria
Where does oxidative phosphorylation occur?
In the inner membrane of mitochondria
Where does electron transport occur?
Inner membrane of mitochondria.
How many reducing equivalents are fed into the electron transport and from where?
Total 12:

Glycolysis (2)
Pyruvate DH (2)
TCA: (8)
NAD(P)H productuion can be assayed how?
By observing the absorbance at 340 nm.
Which complexes pump out protons (and how many) when transferring electrons?
Complex I: 4H
Complex III: 4H
Complex IV: 2H
How many electrons does pump I accept?
2 electrons
Complex I accepts electrons from what?
NADH
Complex I transfers electrons to what?
Coenzyme Q (ubiquinone)
What is the enzyme name for Complex II?
Succinate Dehydrogenase
What does Complex II do?
Make FADH2 and transfers to coenzyme Q.
What is another name for complex III?
Cytochrome b/c1 complex
What does complex III do?
Accepts electrons from coenzyme Q and transfers it to cytochrome c.
What exactly is cytochrome c?
A soluble protein in the INTERMEMBRANE space that transfers electrons from Complex III to Complex IV.
What does complex IV do?
Accepts electrons from cyt c and transfers them to H+ and O2 to make water
What is another name of Complex IV?
Cytochrome oxidase
What is another name for complex I?
NADH Dehydrogenase
5 types of electron/donors.
1.) FMN
2.) Fe-S proteins
3.) cytochromes
4.) coenzyme Q
5.) Cu-Cu or Cu-Fe centers
List off the cytochromes
a
a3
b
c
c1
heme proteins
Name the electron acceptor/donor in complex I.
FMN and Fe-S proteins
Name the electron acceptor/donor in complex II.
FAD and Fe-S proteins
Name the electron acceptor/donor in complex III.
Heme and Fe-S proteins
Name all the electron acceptor/donors in complex IV.
Hemes and Cu centers
Where would you find Fe-S centers?
Complex I, II, and III.
Coenzyme Q is what type of molecule?
Lipid soluble quinone
What agents inhibit electron transfer? Describe specifics.
1. Cyanide (cytochrome oxidase inhibitor)
2. Carbon monoxide (cytochrome oxidase inhibitor)
3. Antimycin A (blocks transfer from cytochrome b to cytochrome c)
4. Rotenone (Prevents e- transfer from Fe-S center to ubiquinone)
What agents inhibit ATP synthase? Describe specifics.
Oligomycin (Inhibits Fo and CFo)
What uncouples electron transfer with phosphorylation?
1. FCCP (targets hydrophobic proton carriers)
2. DNP (targets hydrophobic proton carriers)
What is the overall eq. in OX PHOS?
NADH + H+ + 1/2 O2 --> NAD + H2O
What is the vectoral eq. in OX PHOS?
NADH + 11H+(matrix) +1/2 O2 --> NAD + 10H+(intermembrane) + H2O