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33 Cards in this Set
- Front
- Back
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Where does glycolysis take place?
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Cytosol
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Where does Pentose Phosphate Pathway take place?
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Cytosol
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Where does TCA occur?
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Matrix of mitochondria
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Where does Pyruvate Dehydrogenase occur?
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Matrix of mitochondria
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Where does oxidative phosphorylation occur?
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In the inner membrane of mitochondria
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Where does electron transport occur?
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Inner membrane of mitochondria.
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How many reducing equivalents are fed into the electron transport and from where?
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Total 12:
Glycolysis (2) Pyruvate DH (2) TCA: (8) |
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NAD(P)H productuion can be assayed how?
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By observing the absorbance at 340 nm.
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Which complexes pump out protons (and how many) when transferring electrons?
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Complex I: 4H
Complex III: 4H Complex IV: 2H |
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How many electrons does pump I accept?
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2 electrons
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Complex I accepts electrons from what?
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NADH
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Complex I transfers electrons to what?
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Coenzyme Q (ubiquinone)
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What is the enzyme name for Complex II?
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Succinate Dehydrogenase
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What does Complex II do?
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Make FADH2 and transfers to coenzyme Q.
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What is another name for complex III?
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Cytochrome b/c1 complex
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What does complex III do?
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Accepts electrons from coenzyme Q and transfers it to cytochrome c.
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What exactly is cytochrome c?
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A soluble protein in the INTERMEMBRANE space that transfers electrons from Complex III to Complex IV.
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What does complex IV do?
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Accepts electrons from cyt c and transfers them to H+ and O2 to make water
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What is another name of Complex IV?
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Cytochrome oxidase
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What is another name for complex I?
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NADH Dehydrogenase
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5 types of electron/donors.
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1.) FMN
2.) Fe-S proteins 3.) cytochromes 4.) coenzyme Q 5.) Cu-Cu or Cu-Fe centers |
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List off the cytochromes
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a
a3 b c c1 heme proteins |
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Name the electron acceptor/donor in complex I.
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FMN and Fe-S proteins
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Name the electron acceptor/donor in complex II.
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FAD and Fe-S proteins
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Name the electron acceptor/donor in complex III.
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Heme and Fe-S proteins
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Name all the electron acceptor/donors in complex IV.
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Hemes and Cu centers
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Where would you find Fe-S centers?
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Complex I, II, and III.
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Coenzyme Q is what type of molecule?
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Lipid soluble quinone
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What agents inhibit electron transfer? Describe specifics.
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1. Cyanide (cytochrome oxidase inhibitor)
2. Carbon monoxide (cytochrome oxidase inhibitor) 3. Antimycin A (blocks transfer from cytochrome b to cytochrome c) 4. Rotenone (Prevents e- transfer from Fe-S center to ubiquinone) |
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What agents inhibit ATP synthase? Describe specifics.
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Oligomycin (Inhibits Fo and CFo)
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What uncouples electron transfer with phosphorylation?
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1. FCCP (targets hydrophobic proton carriers)
2. DNP (targets hydrophobic proton carriers) |
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What is the overall eq. in OX PHOS?
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NADH + H+ + 1/2 O2 --> NAD + H2O
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What is the vectoral eq. in OX PHOS?
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NADH + 11H+(matrix) +1/2 O2 --> NAD + 10H+(intermembrane) + H2O
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