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4 Cards in this Set

  • Front
  • Back
What is the Procedure for gene sequence analysis?
• Grow isolates
• Extract DNA
• PCR with primers
• Sequence bands
• Bioinformatics
• Phylogenetic analysis
What are the mechanisms of pathogen diversity?
Factors that influence allele variation in populations
• Population size
• Mutation
• Random genetic drift and selection
• Migration (gene flow)
• Genetic recombination – through sexual and asexual reproduction
What are the means of Plant Pathogen diagnostics/identification
• Koch’s postulates
• Morphology
• symptoms
• spore shape and size, bacterial colony colour, Gram stain
• presence of identifiable features (ie sclerotia)
• culture morphology on specific growth medium
• Biochemical – protein compounds
• Serological
• Molecular – nucleotide sequence differences
What do monocyclic and polycyclic refer to?
Plant disease epidemics can be classified into two basic types, monocyclic and polycyclic, depending on the number of infection cycles per crop cycle.