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10 Cards in this Set
- Front
- Back
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Shikimate Kinase
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Fifth step Enzyme in the Shikimate pathway.
Good target for microbial agents that inhibit the shikimate pathway (Tuberculosis) |
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(1)microbial SK(Mycobacterium tuberculosis Shikimate Kinase
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has a LID domain: important in both substrate binding and catalysis. ),
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(2)Plant SK
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(a)SK
(b)SKL1 (c)SKL2 |
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tDNA mutants of SK
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skl1(white), sk2(green), sk3(green)
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SKL1:
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-orthologous Cluster restricted to plants (Monocot and Dicot)
-neighbour Joining Bootstrap consensus |
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a.) Skl1-3
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(variagated mutant) new class : leaves and cotyledons emerge as albino, green sectors expand with time. -plastoglobule accumulation
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b.) Skl1-8
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(albino mutant)(whiter): anthocyanin accumulation and plastids in leaf are deformed (mutated)
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Glyphosate:
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-Roundup :Inhibits the shikimate pathway essential for life in plants and microbes.
-inhibiting the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), which catalyzes the reaction of shikimate-3-phosphate (S3P) and phosphoenolpyruvate to form 5-enolpyruvyl-shikimate-3-phosphate (ESP). -Inhibition of the EPSP synthase reaction, by glyphosate, is competitive with respect to phosphoenolpyruvate (interacts to shikimate intermediate) |
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Round up Ready:
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EPSP Synthase (Glycine96 → Alanine mutation)
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Four Products from Phenylalanine:
(F.A.I.L) |
(1)Flavonoids,
(2)Anthocyanin, (3)Isoflavonoids. (4)Lignin. |
