Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
10 Cards in this Set
- Front
- Back
Shikimate Kinase
|
Fifth step Enzyme in the Shikimate pathway.
Good target for microbial agents that inhibit the shikimate pathway (Tuberculosis) |
|
(1)microbial SK(Mycobacterium tuberculosis Shikimate Kinase
|
has a LID domain: important in both substrate binding and catalysis. ),
|
|
(2)Plant SK
|
(a)SK
(b)SKL1 (c)SKL2 |
|
tDNA mutants of SK
|
skl1(white), sk2(green), sk3(green)
|
|
SKL1:
|
-orthologous Cluster restricted to plants (Monocot and Dicot)
-neighbour Joining Bootstrap consensus |
|
a.) Skl1-3
|
(variagated mutant) new class : leaves and cotyledons emerge as albino, green sectors expand with time. -plastoglobule accumulation
|
|
b.) Skl1-8
|
(albino mutant)(whiter): anthocyanin accumulation and plastids in leaf are deformed (mutated)
|
|
Glyphosate:
|
-Roundup :Inhibits the shikimate pathway essential for life in plants and microbes.
-inhibiting the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), which catalyzes the reaction of shikimate-3-phosphate (S3P) and phosphoenolpyruvate to form 5-enolpyruvyl-shikimate-3-phosphate (ESP). -Inhibition of the EPSP synthase reaction, by glyphosate, is competitive with respect to phosphoenolpyruvate (interacts to shikimate intermediate) |
|
Round up Ready:
|
EPSP Synthase (Glycine96 → Alanine mutation)
|
|
Four Products from Phenylalanine:
(F.A.I.L) |
(1)Flavonoids,
(2)Anthocyanin, (3)Isoflavonoids. (4)Lignin. |