Reading...
Play button
Play button
Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
12 Cards in this Set
- Front
- Back
|
how is DNA prematurely terminated?
|
addition of dideoxynucleoside triphosphates (ddNTPs)
|
|
|
what do you call when dideoxynucleotides have hydrogen in 3' position and stop DNA synthesis when added to a growing chain?
|
chain terminating nucleotides
|
|
|
what are the methods called (2 diff names)
|
sanger method, dideoxy method
|
|
|
4 _______ taking taking place in 4_______
|
separate DNA polymerization reactions, reaction tubes.
|
|
|
what's added after DNA tamplate to be sequenced were placed in tubes?
|
four types of dNTPs as building blocks of new sequences and DNA polymerase to catalyze reaction.
|
|
|
primer has...
|
radioactive or fluorescent label on 5' end for visualization
|
|
|
4 ddNTPs
|
ddATP, ddGTP, ddCTP, ddTTP
|
|
|
ratio
|
1ddNTP/50 dNTP
|
|
|
reactions tubes will be separated on
|
denaturing polyacrylamide gel and detected by either autoradiography or fluorescence.
|
|
|
what detects color of fragment when electrophoresed thru polyacrylamide gel?
|
laser beam
|
|
|
data print out is called
|
electropherogram
|
|
|
clusters on the phylogenetic tree are called
|
haplogroups, which consist of various haplotypes that are similar and share a common ancestor.
|
