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26 Cards in this Set
- Front
- Back
- 3rd side (hint)
What is the Enterotube 11 System used for |
Rapid identification of organisms in the enterobacteriacae family. GRAM Negative |
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Who many chambers of different types of media? |
12 chambers |
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How many biochemical test is incorporated into simple tube for rapid identification |
15 Chambers |
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What type of bacteria are in the Enterobacteriacae family? |
Gram negative rods/Coccobacillus and Oxidase negative. |
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What happened after incubation? |
A numerical code is resulted then match up with a different kind of organism. |
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production will cause complete separation of the wax overlay from the surface of the media. |
GAS |
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Fermentation of can result in the production of acid or gas |
Glucose |
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What indicates anaerobic conditions in the Enterotube? |
Paraffin wax layer |
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of an amino acid causes the alkaline end product. |
Decarboxylation |
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What are the 3 criteria for a chemical to be considered an antibiotic? |
(1) Chemical substance produced by a microbe. (2) Stops or inhibits the growth of microorganisms. (3) Effective in small does. |
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What is the Kirby Method (plate) panel of test used for? |
Used to test antibiotics to see which one is most effective to treat an organism. |
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What are the standards we follow for the kirby Bauer Test in regards to Muller-Hinton Agar Plate. |
Use nonselective, nondifferential media; that contains starch to absorb toxin that are released by bacteria.
And lose agar to allow for best diffusion of antibiotics on plates. |
Agar type and function. |
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What does the McFarland Standard- measures ? |
The density of the broth agar culture .
Inoculation Standard (kirby bauer test) |
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What is Time frame for Kirby Bauer Test and what degrees? Standard |
24 Hr incubation at 37 degree |
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What family of bacteria can we use the Enterotube system for? |
Enterobacteriacae-gram negative bacilli/coccobacilli |
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Why do we pierced a hole in the last 8 chambers? |
To allow oxygen to circulate through the chamber. |
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Chambers 5,6,7 and 8 fermentation of sugar create what? |
An acidic end product. |
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Chamber 9 |
Not used in class |
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(The Kirby Bauer Method) Zone of inhibition,intermediate, sensitive, resistant. |
Know these |
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What is PPE? |
Personal Protection Equipment |
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What is PPE designed for? |
To protect oneself from infection that could potentially come from dealing with sick patients or pathogens. |
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What are some examples of PPE |
Gloves, Lab Coat, Face Shield and face mask. |
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Two antibiotics are tested for efficiency against a single bacterium species. Antibiotics A and Antibiotic B produce zones of inhibition with the same diameter, how can the bacterium be considered resistant to Antibiotic A but sensitive to Antibiotic B? |
Zone of inhibition can vary with the
diffusibility and the concentration
of the antibiotics. |
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What factors are the Standardized for the Kirby-Bauer method? |
Used of Muller-Hinton Agar,McFarland standard. 24 hrs at 37c incubation. Antibiotic concentration and diffusion rate. |
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Kirby-Bauer test determine sensitivity,intermediate, and resistant . |
Answer |
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What kind of media does Kirby bauer test used? |
Nonselective and nondifferential media |
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