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70 Cards in this Set
- Front
- Back
- 3rd side (hint)
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which gram stain grows on EMB?
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Gram -
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what is the organism in sour milk, buttermilk, and sourcream?
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Streptococcus lactis
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What are the organisms in Kefir, and yogurt?
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Lactobacillus Bul, and Streptococcus Thermophilus
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what is the three organisms in yogurt?
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Lacto Acidophilus, Lacto Bulgaricus, and Strep Thermo
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yogurt, BULGERT.
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what is Lamisil used for?
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Tinea Pedis (ringworm of the nail...
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What is the organism in nail ringworm?
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Trychophyton rubrum
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what is the active ingredient in lamisil?
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Terbinafine HCl
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What does Pneumocystis carinii look like?
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crushed ping pong balls...the lung solid in a liver...in AIDs patients, PCP
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what are the colors of the threemold slide?
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Penicillium-fuschia
Aspergillus-green Rhizopus-pink-red |
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which kind of mold grows on bread?
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Rhizopus
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what kind of technique do you use for identifying Molds?...and which kind of dye do you use?
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Agar block technique...and you use LPCB
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what are the capsulated organisms?
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Klebb, Crypto, EA, Strepto P, and P florescens
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what is the capsule of Streptococcus mutans made out of?
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polysaccharide capsule
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what is important about the polysaccharide capsule around strepto Mutans?
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it prevents ingestion of pneumococci by alveolar macrophages
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what is the blue/green pigment from PA called?
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"pyocyanin"
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What are three antibiotics tyhar are affective against PA?
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flouroquinolones, gentamicin, and imipenem
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what is the oxidase reaction of PA?
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Oxidase +
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What kind of animal's blood is in Media?
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Sheep
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What kind of organisms grow on enriched media (blood, cocoa)
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niccerias, strepps, and haemophillus)
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what does a medium need to support streptococcus pn
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sheep blood, yeast ex, glucose, Mg, and the buffer
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what org grows on Lowenstein-Jensen medium?
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Mycobacterium Tuberculosis..and it grows UBER SLOW!! (Tuber...uber..get it?)
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what is the source of glucose in M-9 media?
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Carbon
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What is the source of Ammonium phosphate in a tub of M-9?
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Nitrogen
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if there is green around a body organism growing on a Blood Agar, what does that mean...
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α hemolysis (partial)
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if there is clearing around a colony growing on a Blood Agar, what does that mean?
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β (complete hemolysis)
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what happens when an organism is catalase positive?
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it gives off bubbles
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Waht does a catalast test do?
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differentiates between gram + cocci
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All _____ and _____ are Catalase -
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Enterococci, and Streps
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All _____ and _____ are Catalase +
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Staphs, and Micrococcus
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what is the only Gram - organism that tests POSITIVE in an OXIDASE test??
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PA
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what are two organisms that are oxidase -
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EC, and KP
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what is the smell that comes from swiss cheese?
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propionic acid from propionic fermentation...holes made from the bacteria Propionibacteria Shermanii
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What is the smell that comes from Sour Cream fermentation?
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lactic acid fermentation...
called HOMOLACTIC fermentation... |
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what organisms is used/produced in Swiss cheese fermentation?
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Streptococcus lactis
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What are the two yeasts used in Sour Dough fermentation?
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Lactobacillus sanfransisco, -heterolactic fermentation
and SACCHAROMYCES... |
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what does clearing indicate on an agar plate?
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starch hydrolysis zone from enzyme amylase taht BS has
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what does the skim milk plate differentiate between?
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BS and EC....
BS- with caseinase to break dwn casein EC-no break down |
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on a Nutrient Gelatin, which three organisms are positive for AA breakdown (solidifies)
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SM, PV, and PM
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what are hte two organisms taht are Indole positive? (cherry red ring)
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EC, and PV
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What is the temperature, and time for the Hot Air Oven?
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150C-180C for 2-4 hours
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what materials are used in teh hot air oven?
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powder, metal, oil, and heat resistant glass
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What is the temp and pressure for hte autoclave?
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15psi, and 121C for 15-30 minutes...used for ALL MEDIA, except M-Ent
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what does ETO stand for?
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Ethylene Oxide
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what is sterilization used for?
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Gassyringes, plastics, Petri dishes
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what is the size of the millipore we saw in class?
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0.45µm filters
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What are the temperatures and times for LTH, and HTST?
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LTH- 63C for 30 mins
HTST- 72C for 15 seconds |
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what is the current organism used to test if pasteurization is working properly?
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Coxiella burnetti
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what kind of milk lasts FOREVER????
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UHT milk
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used now in formaldehyde, acid indicator, and in Lysol,
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Phenol
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denatures proteins and ruins nucleic acid,
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Phenol
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softsoap A
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Triclosan-phenol cell wall and protein
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Dial liquid soap A
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Triclosan-phenol cell wall and protein
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Hibiclens A
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Chlorhexidine gluconate (Chlorine and phenol) cell wall and protein
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prescription oral rinse A
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Chlorhexidine gluconate (Chlorine and phenol) cell wall and protein
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Listerine A
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Alcohol (26.9%) cell wall
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Isopropyl rubbing alcohol A/D
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Alcohol (70%) cell wall
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Scope A
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Alcohol (16.6%) cell wall
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H2O2 A
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3% H2O2 protein and DNA (works well)
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Bactine A
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Benzalkonium Chloride (.13%) alters membrane permeability
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Bleach D
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Chlorine denatures protein
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Baxter-exidine 4 A
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Chlorhexidine gluconate (Chlorine and phenol) cell wall and protein
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Vesphene II D
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Phenol cell wall and protein
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Lysol all purpose cleaner D
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Chlorine and phenol cell wall and protein
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Beta dine A
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iodine (10%) denatures protein
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Lever 2000 A
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Triclosan- phenol cell wall and protein
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Mercuro-chrome A
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Hg-mercury inactivate proteins and metabolic standstill
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tincture iodine A
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alcohol and iodine cell wall and protein
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how many colonies does a countable plate have?
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30-300
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what are the two antimicrobials on this product?
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Bacitracin zinc, and POlymyxin B sulfate
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What is the treatment for oral herpes (HSV 1)?
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Zovirax
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