Lab Safety Midterm

Front 1

"The 10 Worst Lab Accidents" posted on Canvas highlighted an accident where a Physics student working alone at night in a machine shop was killed when:

her hair caught fire from a soldering iron, causing burns over most of her body

a saw cut a major artery, causing her to bleed to death

her hair got caught in a lathe, strangling her

her sleeve caught fire from sparks from a grinder, causing massive third degree burns

she was electrocuted from a frayed wire on an oscilloscope

Back 1

her hair got caught in a lathe, strangling her

Front 2

Pyrophoric materials can ignite when exposed to air.

True

False

Back 2

true

Front 3

As seen in the YouTube video, alkali metals, such as sodium and cesium, can react intensely with water. These types of reactions are:

Endothermic

Exothermic

Back 3

Exothermic

Front 4

OSHA stands for the Occupational Standards and Health Administration.

True

False

Back 4

False

Front 5

The flash point for a flammable substance is higher than the flashpoint of a combustible substance.

True

False

Back 5

False

Front 6

Hydrofluoric acid is commonly used in geology as a way to extract microscopic fossils from sedimentary rock. The rock dissolves in the acid, leaving the acid-insoluble fossils behind. This can cause burns if spilled on the skin. Unless gluconate gel is applied to these burns, the high dose of fluoride could cause:

hypoglycemis

hypocalcemia

anemia

septicemia

hypofluoremia

Back 6

hypocalcemia

Front 7

University labs are not subject to OSHA regulations.

True

False

Back 7

false

Front 8

The written document that outlines employee training, proper lab safety protocols, employee health monitoring requirements, and names a Chemical Hygiene Officer is called a(n):

Laboratory Standard

Safety Data Sheet

Chemical Inventory

Chemical Hygiene Plan

Standard Operating Procedure

Back 8

Chemical Hygiene Plan

Front 9

As seen in the posted YouTube video, when calcium carbide is mixed with water, a flammable gas is produced. This gas is:

Ethylene

Methane

Acetylene

Propylene

Propane

Back 9

Acetylene

Front 10

Acids and bases are both classified as corrosives, so they can be stored together in a corrosion-proof cabinet.

True

False

Back 10

True

Front 11

In the ChemAlert color coding system for storing chemicals, gray signifies:

toxic/health hazard

Corrosive

Flammable

Reactive

General Storage

Back 11

General Storage

Front 12

A dust fire can occur when fine dust particles are exposed to:

water

organics

oxygen

other reactive particles

Back 12

oxygen

Front 13

When a strong oxidizer (such as potassium permanganate) is mixed with an organic compound (such as glycerin), it will start a fire.

True

False

Back 13

True

Front 14

"The 10 Worst Lab Accidents" posted on Canvas highlighted a chemistry lab accident where nickel hydrazine perchlorate exploded when it was mixed with water.

True

False

Back 14

False

Front 15

Match the following toxin category with its appropriate target organ/system:

Hepatotoxin, Pulmonary toxin , Dermal toxin, Nephrotoxin, Neurotoxin

Kidney, Skin, Nervous System tissues, Lungs, Liver

Back 15

Hepatotoxin: Liver

Pulmonary toxin: Lungs

Dermal toxin: Skin

Nephrotoxin: Kidney

Neurotoxin: Nervous System Tissue

Front 16

Five groups of ten mice each were exposed to different concentrations of Chemical X for 8 hours. After eight hours, the number of dead mice per group was counted and plotted on a graph. What is the independent variable on the graph?

number of dead mice

timecon

centration of Chemical X

mouse test group

Back 16

centration of Chemical X

Front 17

A chemical or agent that causes an increased rate of changes in the nucleotide sequences in DNA is specifically called a(n):

allele

mutagen

teratogen

adaptation

carcinogen

Back 17

mutagen

Front 18

Match the following:

Acute effect, Synergistic effect, Cumulative effect

death within 4 minutes from an allergic response to a bee sting

liver damage from combining acetaminophen (Tylenol, etc.) with alcohol

decreased mental ability in a child who regularly eats fish with high mercury content

Back 18

Acute effect: death within 4 minutes from an allergic response to a bee sting

Synergistic effect: liver damage from combining acetaminophen (Tylenol, etc.) with alcohol

Cumulative effect: decreased mental ability in a child who regularly eats fish with high mercury content

Front 19

When storing a chemical, information regarding chemical incompatibilities can be found in Section __?__ of the chemical's Safety data Sheet (SDS).

1, 3, 7, 8, 11

Back 19

7

Front 20

Threshold Limit Values (TLVs) set by the American Conference of Government Industrial Hygienists (ACGIH) are the legal limits/standards for chemical exposure in the workplace.

true

false

Back 20

false

Front 21

The most common occupational route of exposure is:

Dermal

Ingestion

Inhalation

Injection

All of the above occur at the same frequenc

Back 21

Inhalation

Front 22

What is the meaning of the following laboratory warning sign?

Back 22

Corrosive

Front 23

All natural products (from plants, mushrooms, etc.) are safe because they are normally found in nature.

true

false

Back 23

false

Front 24

The AMES test specifically tests for:

carcinogenicity

transmissibility

mutagenicity

teratogenicity

toxicity

Back 24

mutagenicity

Front 25

The study of the incidence of disease within a population is termed:

toxicology

parasitology

epidemiology

pandemicology

ecology

Back 25

epidemiology

Front 26

Which of the following is a non-transmissible disease?

Heart disease

Genital warts caused by the Human Papilloma

Virus (HPV)

The common cold

Salmonellosis acquired from mouth pipetting a bacterial culture in a Microbiology lab

AIDS

Back 26

Heart disease

Front 27

Drinking an amount of water above a certain dose can kill a person.

true

false

Back 27

true

Front 28

In our society, the acceptable rate of death due to chemical exposure at work is:

1 death per 100 people

1 death per 1,000 people

1 death per 10,000 people

1 death per 100,000 people

1 death per 1,000,000 people

Back 28

1 death per 100,000 people

Front 29

Jonas Salk developed the first vaccine against smallpox.

true

false

Back 29

false

Front 30

Risk ranking is also known as:

risk assessment

risk analysis

risk communication

comparative risk assessment

risk management

Back 30

comparative risk assessment

Front 31

The risk of getting killed while riding a motorcycle is about 1 in 1,500. The risk of dying from smoking is about 1 in 750. It is:

twice as likely to die on a motorcycle than it is to die from smoking

twice as likely to die from smoking than it is to die on a motorcycle

equally likely that a person will die from smoking than by riding a motorcycle

20 times as likely to die on a motorcycle than it is to die from smoking

20 times more likely to die from smoking than it is to die on a motorcycl

Back 31

twice as likely to die from smoking than it is to die on a motorcycle

Front 32

It is important to have boys and girls vaccinated against the HPV (human papillomavirus) before they become sexually active because this vaccine can help prevent:

pregnancy

polio

urinary tract infections

certain types of cancer in boys and girls

formation of memory cells

Back 32

certain types of cancer in boys and girls

Front 33

The general public perceives a greater risk from a hazard when it:

is relatively familiar and simple

is viewed as beneficial and necessary

is involuntarily thrust upon them

involves a large number of deaths spread out over time (versus a sudden, catastrophic death toll)

involves a sincere search for alternatives

Back 33

is involuntarily thrust upon them

Front 34

Which of the following is believed to cause microcephaly?

flu virus

H1N1 virus

polio virus

Zika virus

smallpox virus

Back 34

Zika virus

Front 35

Autism has been scientifically shown to be linked to childhood vaccines.

true

false

Back 35

false

Front 36

Which of the following is a cultural hazard?

carbon monoxide poisoning

skiing

earthquake

measles virus

earthquake

Back 36

Skiing

Front 37

The subjective way in which the public views a risk is known as :

risk assessment

risk management

risk perception

risk communication

comparative risk assessment

Back 37

risk perception

Front 38

In order for the herd effect/herd factor to protect a population from a disease outbreak, approximately ___?___ of the population must be immunized.

100 %

95 %

90 %

85 %

75 %

Back 38

90%

Front 39

So far, the Zika virus could be classified as a:

mutagenic agen

tteratogenic agent

carcinogenic agent

a and b

all of the above

Back 39

a and b

Front 40

When discarding a used pipette in a plastic benchtop pipette discard bin, the pipette should be put in the bin:

with the pipette tip down

with the pipette tip up

only after running alcohol through the pipette

choices a & c

choices b & c

Back 40

choices a & c

Front 41

The warning sign pictured above means:

Back 41

biohazard

Front 42

List three different ways in which you can get burned when using an autoclave. BE SPECIFIC!

Back 42

Avoid bumping contains, as they may boil over and burn you

Allow liquids to settle (stop boiling) before mov(same as #1)ing flasks

Avoid touching insides of autoclave, inside of door, or any containers

Front 43

List three standard microbiological practices that you, as a student, would be expected to follow when working in a classroom microbiology lab.

Back 43

1. The laboratory supervisor must enforce the institutional policies that control access to the laborato(Students don't do this.)ry.

2. Persons must wash their hands after working with potentially hazardous materials and before leaving the laboratory.

3. Eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing food for human consumption must not be permitted in laboratory areas. Food must be stored outside the laboratory area in cabinets or refrigerators designated and used for this purpose.

Front 44

Hepatitis B and C are blood-borne pathogens that can be contracted by a needle-stick.

true

false

Back 44

true

Front 45

A broken test tube that is not contaminated should be disposed of in a:

sharps container.

biohazard bag

broken glass discard box

trash can

recycle bin

Back 45

broken glass discard box

Front 46

A self-closing door with locks is required for a Biosafety Level 1 lab.

true

false

Back 46

false

Front 47

Which of the following is/are considered a biohazard?

bacteria

fungus

virus

bacteria and fungus

all of the choices

Back 47

all of the choices

Front 48

Using a biological safety cabinet/hood is an example of a primary barrier.

true

false

Back 48

true

Front 49

Laminar flow hoods and Biosafety cabinets filter air through:

HEPA filters

Micro-Filtration filters

Millipore filters

Whatman filters

Back 49

HEPA filters

Front 50

The use of a hand sanitizer is specifically a form of:

sterilization

antisepsis

disinfection

Back 50

antisepsis

Front 51

Test tubes should NOT be capped tightly when being put:

on a vortex

in a centifuge

in an autoclave

on a vortex and in a centifuge

all of the choices

Back 51

in an autoclave

Front 52

Internal pressure must read "zero" before opening:

a waterbath

a centrifuge

an autoclave

centrifuge and an autoclave

all of the choices

Back 52

an autoclave

Front 53

Healthcare workers that may be exposed to blood-borne pathogens should be vaccinated when possible. For which of the following pathogens is a vaccine available?

Hepatitis B

Hepatitis C

HIV

Hepatitis B and Hepatitis C

all of the choices

Back 53

Hepatitis B

Front 54

The container pictured above is used for the disposal of:

used pipettes

contaminated Petri plates/dishes

broken glass

contaminated sharps

all of the choices

Back 54

contaminated Petri plates/dishes

Front 55

If you have a kitchen grease fire in a pan on a stove, you should turn off the heat source and:

leave the pan on the stove and let the fire burn itself out

pour water on the pan to put out the fire

move the flaming pan outside the house

cover the pan with a lid or a damp towel

panic, scream like a baby, and call for someone else to do something

Back 55

cover the pan with a lid or a damp towel

Front 56

An ABC fire extinguisher can also be used on type D and type K fires.

true

false

Back 56

false

Front 57

List the three parts of the fire triangle.

Back 57

Enough OXYGEN to sustain combustionEnough HEAT to reach ignition temperature.Some FUEL or combustible material

Front 58

What surprised you most when you used a fire extinguisher to "put out a fire"? (The simulation trailer and the "laser" don't count as a surprise.)

Back 58

I was surprised at how difficult it was to aim at first. Holding a fire extinguisher feel awkward to me.

Front 59

Match the following Fire Classes with their Fuel sources:

A, B, C, D, K

------------

ordinary combustible materials (wood, paper, etc.)

flammable liquids

electrical equipment

combustible metals

cooking media (kitchen oil, grease)

Back 59

A:ordinary combustible materials (wood, paper, etc.)

B:flammable liquids

C:electrical equipment

D:combustible metals

K:cooking media (kitchen oil, grease)

Front 60

List each step in the P.A.S.S. method of fire extinguisher use and DESCRIBE each step.

Back 60

Pull the pin

Aim at the base of the fire

Squeeze the top handle

Sweep from side to side

Front 61

When using a fire extinguisher to put out a fire, the spray should be aimed at the top and middle of the fire.

true

false

Back 61

false