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32 Cards in this Set
- Front
- Back
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Media used for primary inoculation of specimen; usually prepared in petri dishes so they can be streaked to obtain isolated colonies of any organisms present |
Primary isolation media |
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Media that has been enriched by the addition of extra ingredients to enhance the growth of fastidious microbes |
Enrichment media |
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What are the 2 types of Enrichment media? |
blood agar, chocolate agar |
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Media used to grow one particular type of bacteria from a mixed culture by inhibiting the growth of the other bacterial species |
Selective media |
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Media used to distinguish between species of bacteria which may look exactly alike or very similar by other methods, such as the Gram stain, or TSA |
Differential media |
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What type of plate will you use for nutrient primary isolation media? |
trypticase soy agar; TSA |
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What type of plate grows many types of bacteria, both gram positive and gram negative? |
trypticase soy agar; TSA |
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What type of plate grows only gram-positive bacteria? |
phenylethyl alcohol agar; PEA |
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What type of media is PEA? |
selective |
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What type of plate grows only gram-negative bacteria? |
MacConkey agar; MAC |
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Because MAC only grows gram-negative bacteria, it is a ____________ media. |
selective |
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How is MacConkey agar used as a differential media? |
To distinguish between lactose-fermenting and non-lactose fermenting bacteria |
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Which selective plate media only grows Staphylococcus bacteria? |
Mannitol salt agar; MSA |
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MSA is a differential media because it distinguishes what 2 things? |
Staphylococcus aureus and other Staphylococcus species |
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If Staphylococcus aureus appears on a MSA plate, what color will the plate be? |
Yellow |
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If there is Staph species on a MSA plate, but it is not Staph aureus, what color is the plate? |
Red |
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Which plate only grows gram-negative bacilli? |
Eosin methylene blue; EMB |
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What does the selective media EMB grow? |
gram-negative bacilli |
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EMB is a differential media because it distinguishes _________ from ___________. |
E. coli from other gram-negative bacilli |
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If there is E. coli present on an EMB plate, there will be a metallic blue-black color with: |
a greenish sheen |
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If the gram-negative enteric bacilli on an EMB plate is not E. coli, it will appear: |
pink or transparent |
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Enrichment plate used to grow a variety of fastidious microorganisms such as Streptococcus |
blood agar; BAP |
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Which plate would you choose to grow Streptococcus? |
BAP; blood agar |
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Which plate would you use to demonstrate different types of hemolysis? |
BAP; blood agar |
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What type of hemolysis shows complete lysis of the red blood cell, appearing totally clear? |
beta hemolysis |
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What type of hemolysis might show up if you were sick, showing an abnormal result? |
beta hemolysis |
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What type of hemolysis exhibits incomplete lysis of red blood cell? |
alpha hemolysis |
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What does alpha hemolysis look like? |
Greenish halo around bacterial colonies |
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What type of hemolysis exhibits no lysis of the red blood cell? |
gamma hemolysis |
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What does gamma hemolysis look like? |
No significant change in the color of agar surrounding the colonies |
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What do you call a culture where all of the cells present in the culture originated from a single cell type? |
pure culture |
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What is the classic method used for isolating a pure culture from a mixed culture? |
streak plate method |
