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23 Cards in this Set
- Front
- Back
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Hemolysis
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Icterus
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Lipemia
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Plasma
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Reference range
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Serum
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Samples
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-Most chemical analysis requires serum -Collection of high-quality samples has a direct effect on results -Analyze within 1 hour after collection(Freezing will preserve the integrity) -Collection before treatment -Collection after fasting (Preprandial sample)is preferred |
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Specific blood collection protocols vary depending on_
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the species, volume needed, method of restraint, and type of sample needed. Blood samples should be collected before treatment since some medications and treatments may affect the results of biochemical testing
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Sample Label
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-Date and time of collection -Owner’s name -Patient’s name -Clinic identification number -Your initials |
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Plasma
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-Fluid portion of whole blood -cells suspended -90% water -10% dissolved constituents (Proteins, carbohydrates, vitamins, hormones, enzymes, lipids, salts, waste materials, antibodies, and other ions and molecules) |
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Serum
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-Plasma with fibrinogen removed -During the clotting process, the soluble fibrinogen in plasma is converted to an insoluble fibrin clot matrix -When blood clots the fluid squeezed out is serum -Centrifuging higher than 2000 to 3000 rpm or for prolonged times may result in hemolysis |
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Serum Separator Tube
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-Serum separator tubes (SSTs) contain gel that forms a physical barrier between serum or plasma and cells -Tube has silica particles on wall to help with clot activation -Some test require you to remove serum immediately after centrifugation |
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Factors Influencing Results
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Hemolysis Lipemia Icterus Certain medications Inappropriate handling |
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Fibrinogen
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plasma protein
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Beer’s law
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End point assay
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Ion-selective electrode
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Kinetic assay
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Optical density
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The degree to which light is transmitted through a medium
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Reflectometer
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Detects light reflected off a test substance
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Spetrophotometer
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-Designed to measure the amount of light transmitted through a solution -Basic components Light source, prism, a wavelength selector, photodetector, and a read-out device |
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