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68 Cards in this Set
- Front
- Back
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Where to dispose glass slide or cover slips |
Disinfectant basin |
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Procedure on contaminated glass test tubes |
Remove tape and place and discard rack |
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What should you do if living material is spilled |
Spray disinfectant on a paper towel and use it to cover the involved area allow the disinfectant to remain on it for 15 minutes put the paper towels in the autoclave bag tell your instructor |
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Chemical spill |
Move away from area and notify instructor immediately if it gets in your eyes continuously flush it with water for 5 minutes |
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Cleaning up die |
Acid alcohol or bleach |
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Emergency number |
480-784-0911 |
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What does a microscope do |
Enlarge an object |
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Total magnification |
Ocular x objective |
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Scanning objective |
4x |
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Low power objective |
10x |
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High power objective |
40x |
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High power oil immersion objective |
100 |
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resolving power |
Wavelength of light 2x numerical aperature |
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Why use oil immersion |
To reduce the amount of light that is refracted or lost |
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Condenser job |
Concentrates the light beam onto the specimens |
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Refractive index |
Determines how much light is bent when entering a material |
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How to get the best Focus |
Use the fine focus knob to adjust for one night and then move the top of the Ring located on the ocular to focus of the other eye |
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Fine focus |
Ajsj |
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Hand hygiene for removing cryptosporidium norovirus and clostridium difficile |
Soap and water |
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Ubiquitous |
Microorganisms are everywhere |
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Pure culture |
A culture containing a single species of microbe |
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Colony |
Many bacterial cells |
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Turbidity |
Cloudiness |
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Media |
Nutrient material suitable for the cultivation of microorganisms can be broth or agar |
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Inoculum |
Sample of a pure culture |
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Why are agar plates inverted |
To prevent condensation from dropping onto the agar surface |
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Why use a pen point of inoculum |
To achieve the dilution needed for separation of one cell from the other |
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What is a basic dye |
Positive ion |
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Acidic dye |
Negatively charged ion |
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Reagents used in the traditional Gram stain procedure |
Crystal Violet stains purple in cell wall iodine combines with crystal violet Acetone alcohol decolorizer absorbs high lipid content Safranin counterstain to stain gram - |
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genera an acid fast stain |
Mycobacterium |
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Why do gram-negative cells lose their primary dye during decolorization |
Because the decolorizer dissolves the lipid content in the cell wall |
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Diseases caused by acid fast bacteria |
Mycobacterium tuberculosis leprosy nocardia asteroids cryptosporidium |
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Acid fast organisms of vs non acid-fast organisms |
Acid fast is purplish pink non acid-fast is blue |
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During the acid-fast staining how is a primary stain forced into acid-fast bacteria |
Heat fixation |
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Why are certain organisms acid fast |
They have a mycolic acid in their cell wall |
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Decolorizer in acid fast vs. Gram stain |
Acid-fast alcohol has acid and the Gram stain decolorizer has acetone alcohol |
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Three reagents used in the acid fast stain |
Carbolfuchsin acid alcohol methylene blue stain |
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3 genes in the P glow plasmid |
BLA gene betalactamase Arac repressor Gfp flourescent protein |
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Bla gene beta lactamase |
Breaks apart ampicillin |
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Arac repressor |
Regualtes transcription of gfp gene |
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Gfp flourescent protein |
Glows under uv light |
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Competent |
Ability to take up the plasmid |
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How to make ecoli competent |
Growth on nutrient agar containing beta-lactam antibiotic ampicillin Nutrient agar containing ampicillin plus the sugar arabinose |
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Regulation mechanism of green fluorescent protein gene expression |
Bla gene and arac are constantly transcribed and translated into protein then the gfp is repressed by the arac however adding arabinose aloows the gfp to be transcribed |
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Gloria agar control |
No glow but there is growth |
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Gloria agar + ampicillin |
No growth no glow because it is resistant to ampicillin |
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Gloria agar + ampicillin P glow |
Growth but no glow because it has no Araginose to transcribe the gfp gene |
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Kirby Bauer test |
Disk diffusion strictly regulated |
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Mic test |
Determines the lowest concentration of an antibiotic that is able to inhibit the growth of a test organism |
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Bacteriophage |
Virus that infects bacteria |
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Lytic cycle |
Viper first open and kill their bacterial host once the appropriate number of viruses has been produced |
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Plaque |
The spot where a virus has landed infected the bacteria and lysed them |
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Why delusions of the T4 virus |
Because there are too many bacteriophage in the original sample |
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Macroscopic vs. Microscopic appearance of mold versus yeast |
Mold as macroscopic used is microscopic |
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What filaments does mold have |
Hyphae |
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Septate |
Hyphal filaments that are separated by a cross wall |
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Aceptate |
Hi filaments not separated by cross walls |
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Saccharomyces cerevisiae |
Budding yeast blue die |
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How to identify mold characteristics |
Hyphy height and color |
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How many spore Arrangements of mold |
3 aspergillus spores like dandelion condiospores Penicillium-fingerlike Rhizopus-in sac like structure sporangia |
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Rhizopus |
Completely round with spores inside |
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Penicillium |
Fingerlike with condiospores at tips |
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Aspergillus |
Dandelion look...ccircle with condiospores all over its surface |
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Saclike structures |
Sporangia |
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Sporangiospores |
Spores in the sac |
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Buds |
In yeast cells |
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Condiospores |
Found in apergillus and pencilium, external growths |
