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16 Cards in this Set

  • Front
  • Back
Mannitol Salt Agar

Mannitol Salt Agar


Pink: basic, eats amino acids= S. Epi


Red: Neutral -no growth


Yellow: Acidic, ferments mannitol= S. Aureus



Eosin Methylene Blue

Eosin Methylene Blue


Green: ferments lactose, Acidic= high coliform -> E.coli


Purple/pink: ferments, Acidic -> coliform


No color: ferments sacrose, non-coliform (usually pathogenic)

Pink: ferments lactose, Acidic (Ecoli)


Yellow: Basic/Neutral ( salmonella)


allow gram neg growth and inhibit gram+

Thioglycollate
Thioglycollate

outcomes:


1.Obligate aerobe: must have oxygen


2.Obligate anearobe: harmed by oxygen


3. Facultative anerobe: do better with oxygen but can survive without


4. Microaerophile: require reduced level of oxygen


5. Aerotolerant: don't need oxygen but can survive if present

Oxidation – Fermentation
Oxidation – Fermentation

Green: neutral, no ferment or oxidation


yellow: acidic, oxidation and fermentation


Blue: Basic, no fermentation

Phenol Red Broth
Phenol Red Broth

Gas production: bubble in tube


Yellow: fermentation, ? deaminase (because of fermentation)


Red(control): No Fermentation (so no gas production), and no deamanse


Pink (orangish): no fermentation, yas deaminase (check derm tube for gas)



Methyl Red TESTRed-Positive
Yellow- Negative
Methyl Red TEST

Red-Positive


Yellow- Negative

Voges Proskauer  Test
Voges Proskauer Test

Red:positive


yellow/grey-green: negative

Catalase Test

Catalase Test

  Decarboxylation Test   
Decarboxylation Test
Kligler Iron Test

Kligler Iron Test

  Motility Test   
 Top of Stab: Motile: - Obligate AreoTop Diffuse: Motile + Obligate Areo Diffuse Stab only:  Motile: -  
Motility Test



Top of Stab: Motile: - Obligate AreoTop Diffuse: Motile + Obligate Areo Diffuse Stab only: Motile: -

  DNase Agar  
DNase Agar

Halo:postive


No Halo: negative


for DNase

Movable gelatin: positive


Solid: negative