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28 Cards in this Set

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What is the purpose of a catalase test?

Test capability to breakdown perozide to water and oxygen

What reagents are used in a catalast test?

Hydrogen peroxide

What does a positive and negative catalase result look like?

Positive: Air bubbles form

Negative: No bubbles

What is the purpose for FTM?

Determine oxygen requirements

What does FTM stand for?

Fluid thioglycollate media

How do you interpret the results of FTM?

What are characteristics that Streptococcus and Staphylococcus share?

Non motile
Non Spore-forming
Facultative anaerobe
Gram Positive

What are identifying characteristics for Staphylococcus?

Cocci in clusters
Catalase +


no or beta hemmolysis
Non fastedious

What are identifying characteristics for Streptococcus?

Cocci in pairs or chains
Catalase -


Alpha, beta, gamma hemolysis


Fastedious

What is the purpose of a gelatin deep?

Determine if organism digests gelatin

What is the enzyme produced for a positive gelatin deep test?

gelatinase

What does a positive or negative gelatin deep test look like?

Positive: liquefied gelatin

Negative: solid

What is the purpose of Glocose fermentation broth?

Determine if organism can ferment glucose

What does a positive or negative Glucose fermentation test look like?

Positive: Turns yellow

Negative: remains red

What is the purpose of a lactose fermentation broth?

Determine if the organism can ferment lactose

What does a positive or negative lactose test look like?

Positive: changes to yellow




Negative: stays red

What is the purose of Lysine decarboxylase?

Test for the presence of lysine decarboxylase

What reagents are used in lysine decarboxylase?

mineral oil

What does a positive or negative lysine decarbozylase test look like?

Positive: changes to purple




Negative: stays yellow

What is the purpose of mannitol fermentation broth?

Determine if the organism ferments mannitol

What does a positive and negative mannitol fermentation broth look like?

Positive: changes color to yellow




Negative: remains red

What is the purpose of a motility deep?

determine if the organism is motile

What is the purpose of a Methyl Red test?

Determine if organism is a mixed acid fermenter

What is the enzyme produced in a positive Methyl Red test?

formic hydrogenylase

What reagents are used in a methy red test?

methyl red indicator drops

What does a positive methyl red test look like?

Positive: broth turns red

What is the purpose of a Voges Proskauer test?

Determine if the organism produces acetoin

What reagents are used in a Voges Proskauer test?

VP A and VP B