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11 Cards in this Set

  • Front
  • Back

Aerotolerance

theability or inability to live in the presence of O2

Obligate Aerobes

MUST have O2 to survive and grow

Facultative Anaerobes

cangrow in the presence OR absence of O2

Aerotolerant Anaerobes

doNOT need O2BUT can survive in the presence of O2

Microaerophiles

survive ONLY when O2 levels are low

Capnophiles

surviveONLY when CO2 levels are high

Obligate Anaerobes

ANY O2 will KILL the bacteria

Concentration gradient

Whenmedia is in a tube the diffusion of O2 back into the broth or agar

Agar Deep Stab

10ml of enriched TSA (allows more types of bacterialspecies to grow)

Methylene Blue

- oxidation-reduction indicator


- blue in the presence of O2


- white in the absence of O2

Most common reason why anaerobicconditions were NOT achieved (i.e., methylene blue indicator strip remainsblue)

•incomplete gasket seal (if an anaerobic jaris used)


•a hole (if a plastic pouch/bag is used)