K - Phys Chem 2

Front 1

Stationary phase for TLC - Silica

Back 1

Polar,

Polar molecules wil have a lower Rf

Front 2

Why do bacteria use D-amino acids in peptidoglycan synthesis?

Back 2

D-amino acids are poorly recognized by other species

(Humans -> L-amino acids used)

Front 3

Characteristics of peptide bonds

Back 3

Low reactivity (stable in physiological conditions)

Planar Molecular geometry

Partial double bond character

Resonance

Front 4

Sanger sequencing method

Back 4

1. Denature DNA with heat

2. Primer annealed to template strand

3. 4 Rxn mixtures set up

- DNA with primer (DNA to be sequenced)

- DNA pol

-dNTP (radiolabeled)

-ddNTP (one type in each mixture)

4. Separate with SDS PAGE

-DNA has negative charge

-DNA added at neg end

-fragment ends in ddATP mixture it was in

Front 5

SDS-PAGE

Back 5

Smaller move further

Front 6

Boyle's law

Back 6

Gas

decrease in volume = increase in pressure

Front 7

An increase in CO2 in the body has what effect on the pH of blood

Back 7

decreases pH

CO2 production--> carbonic acid in blood--> acidic

Front 8

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Back 8

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