Jz Uhcl Cellular Physiology Final Exam 4 1/2

Front 1

--- States of Actin Filaments ---
1)
2)
3)

Back 1

1) Nucleation
2) Elongation
3) Steady State

Front 2

--- States of Actin Filaments ---
Lag Phase?

Back 2

Nucleation

Front 3

--- States of Actin Filaments ---
Growth Phase?

Back 3

Elongation

Front 4

--- States of Actin Filaments ---
Equilibrium Phase?

Back 4

Steady State

Front 5

*** *** is the term used to describe the degradation/construction nature of microtubules.

Back 5

Dynamic instability

Front 6

*** are made out of 13 protofilaments.

Back 6

Microtubules

Front 7

--- Microtubules ---
1) Protofilaments are composed of these subunits...

Back 7

1) α-tubulin and β-tubulin

Front 8

--- Microtubules ---
Composed of # ***...

Back 8

13 protofilaments

Front 9

--- Microtubules ---
1) Though all subunits have ***
2) only the *** subunit gets ***.

Back 9

1) GTP
2) β-tubulin --- hydrolyzed

Front 10

--- Microtubules ---
1) The *** end has the α-tubulin subunit.

Back 10

1) minus

Front 11

--- Microtubules ---
1) The *** end has the β-tubulin subunit.

Back 11

1) plus

Front 12

--- Microtubules ---
1) Newly formed mictotubules are on the *** end
2) where the *** subunit is.

Back 12

1) plus
2) β-tubulin

Front 13

1) *** *** takes place in microtubules.
2) *** *** takes place in actin.

Back 13

1) Dynamic stability
2) Tread milling

Front 14

1) Dynamic stability takes place in ***.
2) Tread milling takes place in ***.

Back 14

1) microtubules
2) actin

Front 15

*** is a drug from the Pacific Yew.

Back 15

Taxol

Front 16

Taxol is a drug from the *** ***.

Back 16

Pacific Yew

Front 17

1) Acrylamide gel fuck up *** ***
2) causing the disease *** ***.

Back 17

1) intermediate filaments
2) peripheral neuropathy

Front 18

*** is the rate-limiting step for microtubule formation.

Back 18

Nucleation

Front 19

γ-TuRC?

Back 19

γ-Tubulin Ring Complex

Front 20

*** acts as a scaffold for α/β-tubulin dimers to begin polymerization.

Back 20

γ-TuRC
γ-Tubulin Ring Complex

Front 21

Nucleation is the rate-limiting step for *** formation.

Back 21

microtubule

Front 22

*** is the first subunit attached to γ-TuRC followed by ***.

Back 22

α-tubulin
β-tubulin

Front 23

--- MicroTubule Organizing Center ---
1) Is also known as the ***.
2) γ-TuRC serve as *** sites.
3) Pair of *** are located in the cytosol.

Back 23

1) centrosome
2) nucleating
3) Centrioles

Front 24

MTOC?

Back 24

MicroTubule Organizing Center

Front 25

Microtubules grow from γ-TuRC of the *** AKA ***.

Back 25

centrosome
MicroTubule Organizing Center

Front 26

*** grow from γ-TuRC of the centrosome AKA MicroTubule Organizing Center.

Back 26

Microtubules

Front 27

--- Microtubules ---
1) Accidental loss of *** *** causes rapid shrinkage and its gain causes rapid growth.

Back 27

1) GTP cap

Front 28

GDP-tubulin dimer forms a *** protofilament.

Back 28

curved

Front 29

GTP-tubulin dimer forms a *** protofilament.

Back 29

straight

Front 30

1) ***-tubulin dimer forms a curved protofilament.
2) ***-tubulin dimer forms a straight protofilament.

Back 30

1) GDP
2) GTP

Front 31

1) *** only walks towards negative end of microtubule...
2) It complexes with *** protein to 'walk.'

Back 31

1) Dynein
2) kinesin

Front 32

Draw the flagellum.

Back 32

Front 33

--- Flagellum ---
1) # outer *** microtubules.
2) # central *** microtubules.

Back 33

1) 9 --- doublet
2) 2 --- singlet

Front 34

--- Flagellum ---
Outer doublet microtubules are made up of...
1) Complete *** microtubule.
2) Incomplete *** microtubule.

Back 34

1) A
2) B

Front 35

--- Flagellum ---
1) How many parts?
2) How many inner parts?
3) How many outer parts?
4) Missing?

Back 35

1) 9
2) 2
3) 6
4) Plasma membrane

Front 36

--- Flagellum ---
Name Inner Parts...

Back 36

(2) Inner Sheath
(2) Central singlet microtubule

Front 37

--- Flagellum ---
Name Outer Parts... inside to outside

Back 37

1) Radial Spoke
2/3) Inner/Outer dynein arm
4) Nexin
5/6) Outer doublet microtubule(complete A / incomplete B microtubule)

Front 38

G-Actin?

Back 38

globulin actin

Front 39

G-actin uses *** protofilaments.

Back 39

13

Front 40

F-actin uses *** protofilaments.

Back 40

11

Front 41

ARP?
ARC?

Back 41

Actin-Related Proteins
Actin-Related Complexes

Front 42

ARC can attach to actin filaments at *** angles.

Back 42

70 degree

Front 43

Actin polymerization is *** faster at *** end than *** end.

Back 43

10x
positive
negative

Front 44

*** is a family of actin-binding proteins which disassembles actin filaments.

Back 44

Cofilin

Front 45

*** gets rid of old actin.

Back 45

Cofilin

Front 46

A *** is something formed out of four sub-units.

Back 46

tetramer

Front 47

1) These two filaments are similar....
2) This filament is different....

Back 47

1) actin --- microtubules
2) intermediate filaments

Front 48

1) α-helical region in ***
2) coiled-coil ***
3) ***(adjective) *** of two coiled-coil dimers
4) # *** packed together end to end
5) # tetramers twisted into a rope-like ***

Back 48

1) monomers
2) dimer
3) staggered tetramer
4) many tetramers
5) eight --- filament

Front 49

*** *** fuck up intermediate filaments and cause peripheral neuropathy.

Back 49

Acrylamide gel

Front 50

1) ALS?
2) AKA ***
3) Disease of the *** ***
4) Usually causes death in # ***.

Back 50

1) Amyotrophic lateral sclerosis
2) Lou Gehrig's disease
3) motor neurons
4) 3 years

Front 51

1) *** is a protein used to determine the source of metastasized cancer cells.

Back 51

1) Keratin

Front 52

1) *** is cancer of the epithelial.
2) *** is cancer of the connective tissue.

Back 52

1) Carcinoma
2) Sarcoma

Front 53

1) Keratins are polymers of *** ***.
2) They are characterized VIA their unique ***.

Back 53

1) intermediate filaments
2) kDa

Front 54

These drugs affect microtubules
1) Colchicine works by ***
2) Vineblastine/Vincristine works by ***
3) Taxol works by ***

Back 54

1) preventing polymerization
2) preventing polymerization
3) stabilizing