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46 Cards in this Set

  • Front
  • Back
--- Stages of Commitments ---
1) ***: Irreversible
2) ***: Reversible
1) Determination
2) Specification
--- Female Gametes ---
1) ***: mature "animal" gamete
2) ***: young mature gamete
3) ***: mature "lower plant/algae" gamete
4) ***: immature egg
1) ovum
2) ovule
3) oosphere
4) oocyte
ZP?
Zona Pellucida
A *** cell will differentiate autonomously ONLY when placed in a neutral environment.
specified
A *** cell will differentiate autonomously EVEN when placed in a different region of an embryo.
determined
Basic modes of commitment
Cell Specification Mechanisms
1) *** specification
2) *** specification
3) *** specification
1) Autonomous specification
2) Conditional specification
3) Syncytial specification
Vertebrate cell specification mechanism...
Conditional
Invertebrate cell specification mechanism...
Autonomous
Insect cell specification mechanism...
Syncytial
--- *** Specification ---
1) Morphogenetic Determinants: *** & *** found
2) in *** cytoplasm causing predetermined commitment.
Autonomous
1) Proteins --- mRNA
2) ovum
***: Mode of commitment involving interactions w/neighboring cells.
Conditional Specification
1) *** *** is the ability of EMBRYONIC cells to change cell fate to compensate
2) for missing parts... and is found in *** Specification.
1) Regulative Development
2) Conditional
Studies on Conditional Specification led to development of 4 techniques involved in experimental embryology...
1) Defect Experiment
2) Isolation Experiment
3) Recombination Experiment
4) Transplantation Experiment
*** Experiment: Portion of embryo destroyed.
Development of i*** embryo is observed.
Defect
impaired
*** Experiment - Portion of embryo removed.
Development of *** embryo & *** part observed.
Isolation
partial -- isolated
*** Experiment: Replace original embryonic part w/a part from different region. Observe embryo development.
Recombination
*** Experiment: Portion of embryo replaced w/portion from different embryo.
Transplantation
--- Sea Urchin Development ---
1) Studied using *** Experiment.
2) Development using *** Specification.
3) Cells studied are from *** *** region.
1) Isolation
2) Conditional
3) Animal cap
--- Sea Urchin Development ---
Skeletal mesoderm cells have *** influence on formation of new *** cells.
restrictive
gut
1) A morphogen specifies many cell types w/ a *** ***.
2) Diffusing from site of synthesis(***) to site of degradation(***).
1) concentration gradient
2) source --- sink
Morphogen gradients are responsible for *** in flatworms.... There are *** gradients.
regeneration
two
1) Middle section of flatworm cut too small results in...
2) Same section cut near head 1st... then tail... results in...
1) two heads
2) one head, one tail
The **** is used to explain how different tissues respond differently to same to morphogen gradient system.
French Flag Analogy
--- Frog EMBRYO Development ---
1) *** created by *** flatworms result in multi-legged adults.
2) Split limb disc w/barrier into 2 layers and...
3) *** is a morphogen is produced in the frog's *** hemisphere.
1) Cysts - Trematode
2) two limbs form
3) Activin --- vegetal
***: 'Grouping' of cells giving rise to a particular organ even when transplanted.
Morphogenetic Field
***: A group of cells whose position and fates are specified with respect to the same set of boundaries.
Morphogenetic Field
--- Morphogenetic Limb Field ---
***: Located in center of disc, normally giving rise to the limb.
Free limb
-- Syncytial Specifications ---
1) Prior to cellularization of ***!!!, specification
2) is being induced through *** between....
3) After cellularization, ***!!! takes over.
1) blastoderm
2) interactions --- cytoplasmic regions
3) Conditional Specification
--- *** Specification ---
Multinucleated Zygote undergoing karyokinesis... No cytokinesis.
Syncytial
1) Bicoid protein is highest at the *** end.
2) Caudal @ *** end.
1) anterior
2) posterior
Some embyros use all three commitment mechanisms... T/F
True
*** specification is a tile mosaic of independent self-differentiated parts.
Autonomous
1) Limb Field is composed of *** areas...
2) Consisting of these areas...
1) four
2) Bottom, Center, Top, Surrounding
--- Sea urchin - 16-cell embryo ---
1) Development of *** *** Region
2) *** *** cells: severely restricted forming ***
3) *** *** were: not restricted forming ***
1) Animal Cap
2) Aggregated paired --- mesoderm
3) Isolated paired --- ectoderm and mesoderm
--- *** Specification ---
Controlled by mRNA/proteins in egg.
Autonomous
--- *** Specification ---
Variable cleavage produces unique cell fates.
Conditional
--- *** Specification ---
Relative position of cells is important...
Conditional
--- *** Specification ---
Invariant cleavage produces identical uniform cell fates.
Autonomous
--- *** Specification ---
Cell type specification precedes cell migration.
Autonomous
--- *** Specification ---
Cell migration precedes or accompanies cell type specification.
Conditional
--- *** Specification ---
Variable cleavage produces no rigid cell fates for particular Nuclei.
Syncytial
*** is when embryonic cells altering their cell fate to compensate for missing part.
Regulative Development
--- Mollusc Trochoblast Cells ---
1) Cells always become *** because
2) they are governed by *** specification
3) through the direction of ***
1) ciliated
2) autonomous specification
3) Morphogenetic Determinants
--- Drosophila Development ---
1) Cells from MIDDLE of leg placed on tip of antennae form....
2) because the cell responded to...
1) CLAW!!
2) its POSITION in gradient
Morphogen MUST demonstate...
1) An ability to...
2) in a way that cell....
1) act directly on cells
2) response varies with concentration
Four techniques used in Experimental Embryology were developed during early studies on *** ***
Conditional Specification