Jv Uhcl Biochem Lab Final Big Qs

Front 1

DNA Plasmid Isolation and Purification w/Silica Beads
STEPS
1. --------
2. *** for ***
3. Discard ALL *** and use ***
4. *** solution. ***.
5. *** solution. ***.
6. *** solution. ***.
7. *** for ***.
8. Transfer *** to *** *** and mix with *** *** ***.
9. Centrifuge for ***.
10. Discard ***.
11. Add *** ***. Centrifuge for ***.
12. Repeat ***.
13. Centrifuge for *** to remove traces of ***.
14. Place *** into a *** *** tube.
15. Add *** *** *** ***. Centrifuge for ***.
16. Label tube and *** at ***.

Back 1

1. Grow cells overnight
2. Centrifuge --- 30 seconds 2x
3. supernatant --- pipette
4. Resuspension solution. Vortexing.
5. Cell Lysis solution. Gentle inversion.
6. Neutralization solution. Gentle inversion.
7. Centrifuge --- 5 minutes.
8. supernate --- Spin Filter --- Quantum Prep matrix.
9. 30 seconds
10. filtrate.
11. Wash Buffer --- 30 seconds.
12. 10 and 11
13. 2 minutes --- Wash Buffer.
14. Spin Filter --- clean new
15. Molecular Biology Grade Water --- 1 minute
16. store --- 20 C

Front 2

DNA Plasmid Isolation and Purification w/Silica Beads
STEPS
VERBS FOR 16 STEPS?
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.

Back 2

1. Grow
2. Centrifuge
3. Discard
4. Add. Vortexing.
5. Add. Gentle inversion.
6. Add. Gentle inversion.
7. Centrifuge
8. Transfer. Add.
9. Centrifuge.
10. Discard.
11. Add. Centrifuge.
12. Repeat 10 and 11.
13. Centrifuge.
14. Transfer.
15. Add. Centrifuge.
16. store

Front 3

DNA Plasmid Isolation and Purification w/Silica Beads
STEPS
1. Grow cells overnight
2. Centrifuge cells for 30 seconds 2x
3. Discard ALL supernatant and use pipette
4. Resuspension solution. Vortexing.
5. Cell Lysis solution. Gentle inversion.
6. Neutralization solution. Gentle inversion.
7. Centrifuge 5 minutes.
8. Transfer supernate to Spin Filter and mix with Quantum Prep matrix.
9. Centrifuge mixture in Spin Filter for 30 seconds.
10. Discard filtrate.
11. Ass Wash Buffer. Centrifuge 30 seconds.
12. Repeat step 10 and 11.
13. Centrifuge 2 minutes to remove traces of Wash Buffer.
14. Place Spin Filter into a clean new tube.
15. Molecular Biology Grade Water. Centrifuge 1 minute.
16. Label tube and store at 20oC.

Back 3

DNA Plasmid Isolation and Purification w/Silica Beads
STEPS
1. Grow cells overnight
2. Centrifuge cells for 30 seconds 2x
3. Discard ALL supernatant and use pipette
4. Resuspension solution. Vortexing.
5. Cell Lysis solution. Gentle inversion.
6. Neutralization solution. Gentle inversion.
7. Centrifuge 5 minutes.
8. Transfer supernate to Spin Filter and mix with Quantum Prep matrix.
9. Centrifuge mixture in Spin Filter for 30 seconds.
10. Discard filtrate.
11. Ass Wash Buffer. Centrifuge 30 seconds.
12. Repeat step 10 and 11.
13. Centrifuge 2 minutes to remove traces of Wash Buffer.
14. Place Spin Filter into a clean new tube.
15. Molecular Biology Grade Water. Centrifuge 1 minute.
16. Label tube and store at 20oC.