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13 Cards in this Set
- Front
- Back
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What are 5 basic laboratory techniques that are used in diagnosis of infectious disease?
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• direct visualization of the organism
• detection of microbial antigen • search for "clues" produced by the host response to specific microorganisms • detection of specific microbial nucleotide sequences • isolation of the organism in culture |
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What lab test is used to visualized Cryptococci?
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• India ink preparation
• uses CSF and will see large capsule surrounding smaller organisms |
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What lab test is used to identify herpesvirus?
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Tzanck prep
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List some disease diagnosed by detection of microbial antigens
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• meningitis
• respiratory tract infection • genitourinary tract infection • Hepatitis B |
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What infectious causes of menigitis are identifiable by latex agglutination?
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• S. pneumoniae
• H. Flu • N. menigitidis • Cryptococcus |
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What are some infectious causes of respiratory tract infection that can be identified by immunofluorescence?
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• Bordetella pertussis
• Legionella pneumophila • influenza virus • RSV • adenovirus |
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Generally, polymorphonuclear leukocytic infiltrate suggest what type of infectious process?
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acute bacterial process
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Generally, lymphocytic infiltrate suggests what type of infectious process?
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a more chronic process seen in viral, mycobacterial, and fungal infections
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Eosinophila suggest what type of infection?
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helminthic infestations (parasitic)
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Granuloma formations suggests what type of infectious process?
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• mycobacterial
• certain fungal infections |
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What is the purpose of control skin tests?
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• to ascertain if the patient can mount a delayed-type hypersensitivity response
• commonly encountered antigens (ex. Candida, mumps, Trichophyton) are used • sometimes a negative skin test may be seen despite active infection in individuals withe depression of cell-mediated immunity (anergy) |
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What are some lab tests that detect microbial nucleotide sequences?
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• polymerase chain reaction
• nucleic acid sequence-based amplification • branched-chain signal amplification |
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How can identifying nucleotide sequences play a role in treating patients with microbial resistance?
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assays can be used to identify resistant organisms and help guide the clinician in selecting treatment regimens
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