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13 Cards in this Set

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What are 5 basic laboratory techniques that are used in diagnosis of infectious disease?
• direct visualization of the organism
• detection of microbial antigen
• search for "clues" produced by the host response to specific microorganisms
• detection of specific microbial nucleotide sequences
• isolation of the organism in culture
What lab test is used to visualized Cryptococci?
• India ink preparation
• uses CSF and will see large capsule surrounding smaller organisms
What lab test is used to identify herpesvirus?
Tzanck prep
List some disease diagnosed by detection of microbial antigens
• meningitis
• respiratory tract infection
• genitourinary tract infection
• Hepatitis B
What infectious causes of menigitis are identifiable by latex agglutination?
• S. pneumoniae
• H. Flu
• N. menigitidis
• Cryptococcus
What are some infectious causes of respiratory tract infection that can be identified by immunofluorescence?
• Bordetella pertussis
• Legionella pneumophila
• influenza virus
• RSV
• adenovirus
Generally, polymorphonuclear leukocytic infiltrate suggest what type of infectious process?
acute bacterial process
Generally, lymphocytic infiltrate suggests what type of infectious process?
a more chronic process seen in viral, mycobacterial, and fungal infections
Eosinophila suggest what type of infection?
helminthic infestations (parasitic)
Granuloma formations suggests what type of infectious process?
• mycobacterial
• certain fungal infections
What is the purpose of control skin tests?
• to ascertain if the patient can mount a delayed-type hypersensitivity response

• commonly encountered antigens (ex. Candida, mumps, Trichophyton) are used

• sometimes a negative skin test may be seen despite active infection in individuals withe depression of cell-mediated immunity (anergy)
What are some lab tests that detect microbial nucleotide sequences?
• polymerase chain reaction
• nucleic acid sequence-based amplification
• branched-chain signal amplification
How can identifying nucleotide sequences play a role in treating patients with microbial resistance?
assays can be used to identify resistant organisms and help guide the clinician in selecting treatment regimens