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28 Cards in this Set
- Front
- Back
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A principle of the antiglobulin test is: |
AHG reacts with human globulin molecules bound to RBCs or free in serum |
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Polyspecific AHG reagent contains: |
Anti IgG and anti C3d |
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Monoclonal anti-C3d is derived from one clone of ____________ cells |
plasma cells |
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What is a clinically significant antibody whose detection has been reported in some instances to be dependent on anticomplement activity in polyspecific AHG? |
Anti-Jka |
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After the addition of IgG coated RBCs (check cells) to a negative AHG reaction during an antibody screen, a negative result is observed. What would be a correct interpretation? |
The antibody screen needs to be repeated |
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RBCs must be washed at least ____ times before the addition of AHG reagent to remove traces of free serum globulins
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3 |
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An in vitro phenomenon associated with a positive IAT is: |
Identification of alloantibody specificity using a panel of reagent RBCs |
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False-positive DAT results are most often associated with: |
Use of refrigerated, clotted blood samples in which complement components coat RBCs in vitro |
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Polyethylene glycol enhances antigen-antibody reactions by: |
concentrating antibody by removing water |
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Solid-phase antibody screening is based on: |
adherence |
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A positive DAT may be found in which of the following situations> |
HDN |
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What do Coombs' control cells consist of? |
Type-O positive cells coated with anti-D |
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What method required the use of check cells? |
LISS |
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Which factor can affect AHG testing, yet is uncontrollable in the lab? |
antibody affinity |
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If you had the authority to decide which primary AHG methodology to utilize at your lab, which method would you choose based on the knowledge that the majority of the staff are generalists? |
solid phase or gel |
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A 27 year old group O mother has just given birth to a group A baby. Since the mother has IgG anti-A in her plasma, it is likely that the baby is experiencing some in vivo RBC destruction. What method would be most effective at detecting the anti-A on the baby's RBCs?
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DAT using gel
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The _______________ test is used to detect RBCs sensitized by IgG alloantibodies, IgG antibodies and complement components |
antiglobulin |
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________ reagents containing anti-IgG are needed for the detection of IgG antibodies because the IgG monomeric structure is too small to directly agglutinate sensitized RBCs |
AHG reagents |
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Polyspecific AHG sera contain antibodies to human _______ and the _________ component of human complement |
IgG; C3d |
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Monospecific AHG sera contains _____ antibody specificity: either anti-IgG of antibody to anti-C3b-C3d |
1
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Classic AHG sera (polyclonal) are prepared by injecting human globulins into ___________ and an immune stimulus triggers production of antibody to human serum |
rabbits |
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Hybridoma technology is used to produce ____________________ antiglobulin serum |
monoclonal |
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The ________ detects IN VIVO sensitization of RBCs with IgG or complement components. Clinical conditions that can result in a positive result include HDN, HTR, and AIHA. |
DAT |
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The ___________ detects IN VITRO sensitization of RBCs and can be applied to compatibility testing, antibody identification, RBC phenotyping, and titration studies |
IAT |
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A positive DAT is followed by a _____________ using monospecific anti-IgG and anti-C3d to determine the specific type of protein sensitizing the RBC |
DAT panel |
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____________ should be used to collect blood samples for the DAT to avoid in vitro complement attachment associated with refrigerated clotted specimens |
EDTA |
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LISS, PEG, polybrene, and albumin can all be used as enhancement media for ________ testing
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AHG |
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Conventional tube testing, gel technology, enzyme linked technology, and solid-phase testing are available methods to use in __________ testing |
AHG |
