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46 Cards in this Set
- Front
- Back
- 3rd side (hint)
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Streptococcus pyrogenes
Disease |
Pharyngitis Skin infections |
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S. pyrogenes
How many diseases? |
2
List |
Pharyngitis Skin infections |
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S. pyrogenes
2 diseases |
Pharyngitis Skin infections
Does it cause other diseases? |
Y |
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S. pyrogenes
other diseases Cause |
Host response to infection
Name of this type of diseases? |
S. pyrogenes sequelae |
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S. pyrogenes
Other diseases Caused by host response to infection
Name of this type of diseases? |
S. pyrogenes sequelae
Occur due to host response to infection
Presence of bacteria? |
Absence (cleared) |
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S. pyrogenes sequelae
bacteria? |
Absence (cleared) |
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S. pyrogenes sequelae
Occur due to host response to infection, after bacteria have been cleared
Diseases? |
Acute rheumatic fever
Glomerulonephritis |
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S. pyrogenes sequelae
How many diseases there are? |
2
List |
Acute rheumatoid fever
Glomerulonephritis |
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S. pyrogenes sequelae
Diseases _______________ Glomerulonephritis |
Acute rheumatoid fever
Inflammation of the ______(organ) After ________ (disease) Abs or cell-mediated response cross-reacting with cardiac tissue. |
heart Pharyngitis |
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S. pyrogenes sequelae
Disease: acute rheumatoid fever
Inflammation of the ____ After ________ Abs or cell mediated response cross reacting with _____ tissue. |
____________ of the heart _______ pharyngitis ____ or ______ response cross-reacting with cardiac tissue. |
Inflammation of the heart After pharyngitis Abs or cell mediated response cross-reacting with cardiac tissue |
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S. pyrogenes sequelae
Disease Acute rheumatic fever ____________ |
Glomerulonephritis
______ dysfunction After _____ or ______ ______ Ab-strep Ag complexes deposited in glomeruli of _____ |
Kidney Pharyngitis or skin infections kidney |
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S. pyrogenes sequelae
Glomerulonephritis
________ dysfunction After ________ or _______ Ab-strep ag complexes deposited in glomeruli of ______ |
Glomerulonephritis
Kidney _______ ______ pharyngitis or skin infections Ab-Strep Ag _____ deposited in ______ of kidney |
Kidney dysfunction after pharyngitis or skin infections
Ab-strep ag complexes deposited in glomeruli of kidney |
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S. pyrogenes sequelae
Why immunodiagnosis is needed? |
___________ is needed because bacteria is likely to have been cleared |
Immunodiagnosis |
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S. pyrogenes sequelae
Immunogiagnosis is needed because bacteria is ____ to have been ______ |
____________ is needed because _____ is likely to have been cleared. |
Immunodiagnosis is needed because bacteria is likely to have been cleared. |
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Abs used to identify/indicate S. pyrogenes infection
General name |
Anti-streptococcal Abs
How many there are? |
3 |
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3 Anti-Streptococcal Abs
List |
ASO (Full name) Anti-Dnase B Anti-hyaluronidase |
Anti-strepolysin O |
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3 Anti-Streptococcal Abs
List
_______ Anti-Dnase B Anti-hyaluronidase |
ASO
How many methods there are? |
2 |
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ASO
Full name How many methods there are? |
Anti-streptolysin O
2 methods
List |
Current Traditional |
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ASO
2 methods
List |
________ (Principle) Traditional |
Latex agglutination |
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ASO
Current method
Principle |
Latex agglutination
Name of the method |
Nephelometry |
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ASO
Current method: _________ Principle: Latex agglutination |
Nephelometry
Advantages |
Automated Rapid |
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ASO
Current method: Nephelometry Principle: latex agglutination
Advantages |
Nephelometry
Automated Rapid
Measurement? |
Quantitative |
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ASO
Current method: Nephelometry
Measurement? |
Quantitative |
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ASO
Current method: nephelometry
Procedure |
Pt serum + Purified recombinant streptolysin
Product? |
Immune complexes |
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ASO
Current method: nephelometry
Immune complexes vs. light scatter |
+ Immune complexes increase light scatter, and the amount can be _____ |
measured |
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ASO testing
Current method ____________ |
Traditional method
Procedure |
Diluted Pt serum+reagent streptolysin O
Incubate
+ Human type O RBCs added
Incubate
Observation |
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ASO testing
Traditional method
Procedure |
Diluted Pt. serum+ Reagent Streptolysin O
Incubate + Human Type O RBCs Incuabte
Observation, description? |
Hemolysis: (-)ASO
No hemolysis: (+) ASO |
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ASO testing
What is determined? |
Titer
Def. |
Reciprocal of highest dilution with no hemolysis |
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Rickettsia
Diseases
How many there are? |
3
List |
Rocky Mt. spotted fever
Endemic and epidemic typhus |
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Rickettsia
3 diseases
List |
Rocky Mt. Spotted fever
Endemic and epidemic typhus |
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Rickettsia
3 diseases
_______________ Endmeic typhus Epidemic typhus |
Rocky Mt. spotted fever
Pathogen |
R. rickettsil |
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Rickettsia
3 diseases
Rocky Mt. Spotted fever ______________ Epidemic typhus |
Endemic Typhus
Pathogen |
R. typhi |
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Rickettsia
3 diseases
Rocky Mt. Spotted fever Endemic Typhus _____________ |
Epidemic typhus
Pathogen |
R. felis |
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Rickettsia diagnosis
Types of test How many there are? |
2
List |
Current Traditional method |
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Rickettsia diagnosis
2 types of test
List |
Current Traditional method |
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Rickettsia diagnosis
_____________ Traditional method |
Current method
Test? |
IFA test |
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Rickettsia diagnosis
Current method Test |
IFA test
______ standard
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Gold standard |
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Rickettsia diagnosis
Current method: IFA test _______ standard |
IFA test Gold _______ |
standard |
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Rickettsia diagnosis
Current method: IFA test Gold standard
Reagent? |
IFA test Whole bacterium
Specificity? |
Organism-specific |
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Rickettsia diagnosis
IFA test
Specificity |
Organism-specific
IFA test
Procedure |
Bacterium fixed on slide + Pt. serum (Ab?) + Flourescently labeled Anti-Ig
Observation |
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Rickettsia diagnosis
IFA test
What is in question? |
Ab
IFA test Procedure |
Bacterium fixed on slide + Pt. serum + Flourescently labeled Anti-Ig
Observation |
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Rickttsia diagnosis IFA test
Bacterium fixed on slide + Pt. serum |
Observation and results |
Flouresence: + Ab No flouresence: - Ab |
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Rickettsia Diagnosis
Current method _____________ |
Traditional method
Name |
Weil-Felix test |
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Rickettsia diagnosis
Traditional method
Name |
Weil-Felix test
What is being detected? |
Agglutination |
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Rickettsia diagnosis
Traditional method: Weil-Felix test
What is being detected? |
Agglutination caused by what? |
Cross rxn between
anti-Rickettsia Abs in the Pt. serum and reagent Proteus species Ag |
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Rickettsia diagnosis
Traditional method: Weil-Felix test
Agglutination is caused by __________ between anti-Rickettsia Abs in Pt. serum and reagent Proteus species antigens. |
___________ is caused by cross reactivity between _____________________ in _____ and _______________. |
Agglutination is caused by cross reactivity between anti-rickettsia Abs in pt. serum and reagent proteus species antigens |
