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20 Cards in this Set

  • Front
  • Back
treatment with papain generates what?
2 Fab segments; each a Ag binding domain
what is a Fab segment?
gen by papain treatment
consists of 1 each:
Vh, Vl, Ch1, Cl1
treatment with pepsin generates what?
1 F(ab)2'; cleaves past disulfide bond that keeps heavy chains together
what is a F(ab)2' segment?
gen by pepsin treatment
consists of 2 each:
Vh, Vl, Ch1, Cl1
where does C1q (classical complement path) bind?
Ch2 domain
how many CDRs are present on 1 Ig?
6 CDRS: 3 on Vl, 3 on Vh
what is the significance of beta pleated sheets?
provide a stable core for variable regions; holds CDRs in position
what are the approx AA ranges for the 3 CDRs?
CDR1: 20-30
CDR2: 40-50
CDR3: 90s
what are the prominent intermolecular forces b/w Ag and Ig?
Van der Waals
how do Igs demonstrate flexibility?
large shift in conformation after Ag binding
when binds Ag, wiggles and sets up lipid rafts-> signal transduction
what is the significance of the hinge region?
allows for flexibility of variable region;
can bind 2 molecules of same Ag (bivalent Ig)
what determines the Ig class?
heavy chain
what determines that function of the Ig (physiologic and biologic properties)?
constant regions
what is the fxn of glycosylation and where does it occur on an Ig?
fxn: protect fxnal sites (ex: complement binding site Ch2);
protect interchain bonding sites;
incr hydrophilicity
located only in CONSTANT regions
what are some features of the IgM hexamer?
no J chain
not in secretions
high levels in some autoimmune diseases
what is the bramble receptor?
receptor that moves Ig across endothelium into extracellular spaces;
receptor for transplacental transfer
what is the mxn of the bramble receptor?
receptors located on endothelium (lumenal side)
2 R bind 1 Ig(G)
complex is endocytosed
crosses endothelium to enter tissue
receptor remains in tact
what is the poly-Ig receptor?
binds polymeric Igs (A,M); req J chain
receptor moves Ig from tissue to lumen (secretions)
what is the mxn of the poly-Ig receptor?
located on lamina propria side of epithelial cell;
1 receptor binds 1 Ig(A) via interaction with J chain;
complex endocytosed->lumenal side;
IgA dimer rel with secretory component of receptor attached to C-region via disulfide bonds;
secretory component protects IgA from proteolysis and stabilizes in lumen
what is the Ig superfamily?
molecules that contain at least 1 Ig domain
involved in cell-cell recog
ex: CD3 in TCR, MHC, CAM