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33 Cards in this Set
- Front
- Back
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Biuret Test
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Contatins Copper Sulfate which reacts with peptide bonds to produce a Violet color.
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Solution=Water
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Color of Biuret= lt. blue
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Solution=Egg White
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Color of Biuret= Violet
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Solution=Potato
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Color of Biuret= lt. blue
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Solution=Vegetable Oil
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Color of Biuret= lt. blue
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What chemical property differentiates a protein from other organic molecules?
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Hydrogen Bonds
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What color does the solution turn when it test positive for protein?
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Violet
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Why does the Biuret Test work specifically with proteins?
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Because it contains copper sulfate and reacts with the peptide bonds.
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Hydrophobic
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Water insoluble, or water hating.
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Hydrophillic
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Water soluble, or water loving.
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Conformation
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The formation of a three dimensional shape determined by the arrangement of the amino acids as they fit together.
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Denatured
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The disruption of the conformation by changes in the proteins natural environment.
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Water+Egg White
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Cloudy, lt purple, protein present.
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Water+Egg White
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Cloudy, DARK purple, protein present.
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Acid Ethynol+Egg White
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White, lt purple, protein present.
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Acid Ethynol
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Effects the appearsnce because Acid naturally denatures protein.
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After Denaturation
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Protein is still a protein and poly peptides are still present.
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What is the effect of denaturation?
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It changes the shape of the Tertiary structure and can no longer be useful.
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How do you test for the presence of starch?
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With IKI
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What color does the IKI turn when starch is present?
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Blue/Black
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What color is the IKI?
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orange/yellow
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What type of molecule associates with the IKI?
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Starch or a Polysaccaride.
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Will the IKI work with any type of mono or disaccaride?
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No because Strach has a specific coiled structure that binds with the IKI.
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What does IKI stand for?
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Iodine Potassium Iodide.
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Benedicts tests for?
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The presence of a disaccharide.
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What color is if the test is negative?
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Blue
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What if its positive?
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Orange
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Hydrolysis of Starch is the break down of a...
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Polysaccharide into a disaccaride.
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Benedicts test results in
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a positive effect for reducing polysccarides.
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What test could you perform to prove the presence of protein?
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Benedicts
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What property of enzymes makes thems susceptible to denaturation?
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The hydrogen bonds.
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Sudan II is used for?
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A test for nonpolar, lipophillic molecules.
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When Sudan II is used as a solvent and test positive it turns
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Red, but is naturally an orange color.
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