Hydrateam Test A

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Manitol Salt Agar
Selective for Halotolerance
Differential in use of mannitol
Differential in pH

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Eosin Methylene Blue Agar
Selective against Gram (+) (b/c of eosin Y and methylene blue dyes)
Differential for Gram (-) that can ferment lactose & sucrose and those who cannot

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Pseudomonas Isolation Agar
Selective for Pseudomonas b/c of Triclosan (a phenolic)

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Phenylethyl Alcohol (PEA) agar
Selective against Gram (-) b/c of phenylethyl alcohol

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DNAse Agar
Differential for release of enzyme exonuclease b/c of free DNA in the medium

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Blood Agar
Differential for hemolytic ability
--if can completely lyse = beta hemolytic
--if can partially lyse = alpha hemolytic
--if cannot lyse = gamma hemolytic

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Skim Milk Agar
Differential for release of exoenzyme casease

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Starch Agar
Differential for production of exoenzyme amylase

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Skim Milk Agar
Differential for release of exoenzyme casease

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Starch Agar
Differential for production of exoenzyme amylase

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Catalase Test
Production of the enzyme catalase (peroxidase)

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Kirby-Bauer Antibiotic Sensitivity Test
or Disk Diffusion Test
Tests for an organism's senstivity to various antibiotics

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MPN Test - Most Probable Number of Coliforms in water samples
Uses Lactose broth to test for the presence of indicator organisms called fecal coliforms which then shows fecal contamination of water.

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Oxidase Test
Tests for aerobic organisms that use cytochrome oxidase (cytochrome C) intheir respiratory chains

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Slide Agglutination or Serological Testing
Brand name: "Staphloslide"
Confirms the specific presence of Staphylococcus aureus

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Sugar (Carbohydrate) Utilization Broths
Tests for the use or fermentation of sugars

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Sugar (Carbohydrate) Utilization Broths
Tests for the use or fermentation of sugars

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Citrate UtilizationTest
Identifies organisms capable of using citrate as a sole carbon source
Differential for Enterobacteriaceae
Good way to seperate fecal coliforms (neg) from non-fecal (pos)

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Citrate UtilizationTest
Identifies organisms capable of using citrate as a sole carbon source
Differential for Enterobacteriaceae
Good way to seperate fecal coliforms (neg) from non-fecal (pos)

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Deaminase (Phenylalnine) Test
Checks for ability to deaminate the amino-acid phenylalanine to form phenylpyruvic acid (PPA) and ammonia

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Deaminase (Phenylalnine) Test
Checks for ability to deaminate the amino-acid phenylalanine to form phenylpyruvic acid (PPA) and ammonia

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Indole Test of SIM Test
Identifies members of the Enterobacteriaceae family by checking for indole production from tryptophan in SIM agar

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Indole (right) and Sulfate Reduction (left) tests of SIM Test
Identifies members of the Enterobacteriaceae family by checking for indole production from tryptophan in SIM agar (red ring) and sulfate reduction (black precipitate)

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Indole and Sulfate Reduction tests of SIM Test
Identifies members of the Enterobacteriaceae family by checking for indole production from tryptophan in SIM agar (red ring) and sulfate reduction (black precipitate)
Left Tube is negative for both.

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Urea Hydrolysis Broth
Differential for organisms that produce the enzyme urease and break down urea to ammonia and CO2.

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Urea Hydrolysis Broth
Differential for organisms that produce the enzyme urease and break down urea to ammonia and CO2.

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TTC Motility Test
Differentiates between organisms that are motile or not
Tetrazolium salt turns red where bacterial growth is located.

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SIM Motility Test
Differentiates between organisms that are motile or not
Uses SIM agar

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Nitrate Reduction Test
Differential for organisms that can reduce nitrate using nitrate reductase.
Four different levels of nitrate reduction are possible.

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What is the name of this test?
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Nitrate Reduction Test
Differential for organisms that can reduce nitrate using nitrate reductase.
Four different levels of nitrate reduction are possible.

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Nitrate Reduction Test
Differential for organisms that can reduce nitrate using nitrate reductase.
Four different levels of nitrate reduction are possible.

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MR-VP Test (Methyl Red & Voges-Proskauer)
One broth does two tests as part of IMVIC series for differentiation of Enterobacteriaceae. Culture is split into two tubes for the two tests. The pictured test is the MR which determines the fermentation of glucose into mixed acids. Left is negative and right is positive.

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What is the name of this test?
What does it test for?

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MR-VP Test (Methyl Red & Voges-Proskauer)
One broth does two tests as part of IMVIC series for differentiation of Enterobacteriaceae. Culture is split into two tubes for the two tests. The pictured test is the VP which determines the fermentation of glucose into acetone prior to 2,3-butanediol alcohol. Left is negative and right is positive.

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What is the name of this test?
What does it test for?

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Nitrate Reduction Test
Differential for organisms that can reduce nitrate using nitrate reductase.
Four different levels of nitrate reduction are possible.

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What is the name of this test?
What does it test for?

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MR-VP Test (Methyl Red & Voges-Proskauer)
One broth does two tests as part of IMVIC series for differentiation of Enterobacteriaceae. Culture is split into two tubes for the two tests. The pictured test is the VP which determines the fermentation of glucose into acetone prior to 2,3-butanediol alcohol. Left is negative and right is positive.

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What is the name of this test?
What does it test for?

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Slide Agglutination or Serological Testing
Brand name: "Staphloslide"
Confirms the specific presence of Staphylococcus aureus

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What is the name of this test?
What does it test for?

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Indole and Sulfate Reduction tests of SIM Test
Identifies members of the Enterobacteriaceae family by checking for indole production from tryptophan in SIM agar (red ring) (none is this example) and sulfate reduction (black precipitate) from the enzyme cysteine desulfurase.