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9 Cards in this Set
- Front
- Back
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adenovirus |
- DNA virus - The virus was first isolated from adenoids - Not a severe pathogen - Contains proofreading mechanisms |
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Purposes for studying adenoviruses |
- As a tool for basic biological studies - As a gene delivery vector - As a disease pathogen |
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Adenovirus structure |
- Non-enveloped isohedral capsid with 20 triangular faces - Linear double stranded DNA - Diameter = 70-90nm - Knobbed fibers protruding from each of 12 vertices - Contains 11 proteins |
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2 major proteins for adenovirus |
- Hexon protein (make up 20 triangular faces) - Penton protein (vertices) - Both are located on capsid |
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Knobbed fibers |
- Located on penton protein - Make contact with cellular receptor protein (CAR) to initiate infection - Distribution determines virus tropism (target specificity) - Toxic to cells because it inhibits protein synthesis |
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Philip Sharp ad Richard Roberts |
- First discovered RNA splicing in 1977 at the same time - Share the nobel prize |
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RNA splicing |
- A process in which spliceosomes modify pre mRNA and remove introns while joining exons during transcription - Introns may contain regulatory sequences |
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Alternative splicing |
- Also known as differential spicing - First discovered in adenoviruses - regulatory process in which some exons are spliced out --> results in a single gene coding for multiple proteins. |
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concantamer |
long, continuous DNA molecules which contains multiple copies of the same DNA sequences linked in series |
