Ht Exam 1

Front 1

Microscopic examination of stained slides shows bacilli on, but not in, the tissue sections. The most probable cause of this contaminant is that:

Back 1

the water bath was not cleaned after previous use

Front 2

A tissue section was not initially placed in the fixative required for the staining procedure. However, after deparaffinization and rehydration, sections frequently can be stained if they are:

Back 2

post-fixed in the appropriate fixative prior to staining

Front 3

Microscopic examination of an H&E-stained section fixed in formalin shows marked nuclear bubbling. One most often sees this artifact if the specimen is processed following:

Back 3

underfixation

Front 4

Microscopic evaluation of H&E-stained sections from a surgically removed small bowel specimen show an absence of much of the epithelium in otherwise normal tissue. This most likely resulted from:

Back 4

delayed fixation

Front 5

Fixatives are classified as additive because of the:

Back 5

addition, or binding of the fixative, to tissue proteins

Front 6

Fresh, unfixed tissue can be stored safely for a short time by:

Back 6

wrapping it in saline-moistened gauze and refrigerating

Front 7

Stock neutralized formain is prepared in the laboratory by storing the solution over a layer of calcium carbonate. The solution withdrawn from this stock container will:

Back 7

become acidic

Front 8

Zenker solution contains:

Back 8

mercuric chloride, potassium dichromate, and acetic acid

Front 9

Carnoy solution is a combination of which chemicals?

Back 9

chloroform, glacial acetic acid, and absolute alcohol

Front 10

B-5 fixative contains:

Back 10

mercuric chloride, sodium acetate, and 37%-40% formaldehyde

Front 11

Mercuric chloride pigment may be removed from tissue by:

Back 11

treating sections with an iodine-sodium thiosulfate sequence

Front 12

Organic solvents should be disposed of by:

Back 12

collecting them in waste containers for future disposal of distillation

Front 13

Dehydrating tissues in graded alcohols of increasing concentrations is superior to using absolute alcohol only because it will:

Back 13

cause less distortion of the tissue

Front 14

Xylene functions as a(n):

Back 14

clearing agent

Front 15

The best method for preparing tissue for enzyme demonstration is:

Back 15

unfixed frozen sections

Front 16

Which of the following is necessary to ensure a straight ribbon?

a) horizontal sides of the block must be parallel
b) veritcal sides of the block must be parallel
c) horizontal and vertical sides of the block must be parallel
d) the microtome heel must be rotated rapidly

Back 16

a) horizontal sides of the block must be parallel