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13 Cards in this Set
- Front
- Back
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Hematoxylin & Eosin
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Hematoxylin- dark blue stain
Nucleic acids stain dark, all nuclei are always going to be dark purple or black. EOSIN- less specific than hematoxylin, stains lots of stuff |
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Hematoxylin
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Blue
Basic anything that stains with Hematoxylin is Basophilic (stains acids) Positive charge |
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Eosin
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is an acidic dye
carries a negative charge Stains “mostly basic” components red or pink. structures that stain with eosin are called acidophilic or eosinophilic. |
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_____pushes stain away- no stain
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Lipid
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____ _____ ____are very clear because they are compacted into lipid vacuole
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Steroid producing cells
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What likes to circle around common ducts in an acinar approach?
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EXOCRINE PANCREAS
-Zymogen granules |
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• The nucleus of the cell appears _____in an H/E because of the heterochromatin (ionized phosphate) that resides within it.
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blue
DNA and RNA stain BLUE with HEMATOXYLIN |
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ELASTIC STAIN
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Can Prove cause of death
Purple is elastin |
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Silver stain-
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RETICULAR FIBERS
Type III collagen mostly ordered at autopsy |
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Connective Tissue Stain
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Takes everything that collagen (type1 -18)
color: light blue/ tourqoise blue Used when: Fibrotic liver or anytime connective tissue is where it shouldnt be. |
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Periodic Acid Schiff Stain stain what subcellular components (2) with what functional group?
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Stains glycogen & carbohydrates with aldehyde
Example: BASEMENT MEMBRANES stain magenta because of their proteoglycans |
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Alcholoic chirrhosis
pathogenesis |
Alcohol coming from the gut first makes contact with the hepatocytes nearest the protal tirad, therefore affecting these liver cells first
The body makes collagen in response to injury because it’s the fastest thing it can synthesize |
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PAS stain highlight what cellular components in what color?
what can this tell you (3) |
highlights the basement membrane pink
which can tell you filtration, diffusion, or connective qualities ex: Glycocalyx/ microvillis |
