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38 Cards in this Set
- Front
- Back
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Hematoglobin is measured as _______ |
Oxyhemoglobin |
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Indirect methods of measurement convert hemoglobin into these compounds |
Acid hematin Cyanmethemoglobin Alkali hematin Carboxyhemoglobin |
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What is used in acid hematin method to convert hemoglobin into acid hematin |
0.1N HCl |
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The yellowish brown solution is compared to the color standard in the _______ |
Comparator block |
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What pipette is used in acid method hematin method |
Sahli's pipette |
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How many mL of blood was used during the acid hematin method |
0.02 mL |
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What units can be used in reporting of the results |
gm% gm/dL gm/ 100mL (CU) gm /L (SI) |
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Reagent used in Cyanmethemoglobin method |
Drabkin's reagent |
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Composition of drabkin's reagent |
Sodium bicarbonate Potassium cyanide Potassium ferricyanide |
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Component of drabkin's reagent that converys iron in yhe hemoglobin from ferrous to ferric state |
Ferricyanide |
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Results into conversion of iron from ferrous to ferric state |
Methemoglobin or Ferrihemoglobin |
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This combines to methemoglobin to form Cyanmethemoglobin |
Potassium cyanide |
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Best method of manual hemoglobin determination |
Cyanmethemoglobin |
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The only compund that is not converted by Cyanmethemoglobin method |
Sulfhemoglobin |
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Tube used in Cyanmethemoglobin method |
Wassermann tubes |
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On what absorbance does the method is measured |
540mm |
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Formula for Cyanmethemoglobin |
Au x Cu = As x Cs |
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Advantage of Cyanmethemoglobin |
1. The pigment Cyanmethemoglobin is stable even in a diluted solution 2.the method measures all the hemoglobin except sulfhemoglobin 3. Certified methemoglobin standard is commercially avai6 4. The spectral curve of Cyanmethemoglobin allows the useof different types of spectrophotometers |
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Direct matching of colorimetric methods in hemoglobin determination |
1. Tallquist scale 2. Dare Hemoglobinometer 3. Alkali hematin |
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This methodis bqsed 9ncolor comparison. It uses a very simple chart consisting of different hues of red as a comparator. The color of slightly dried blood on the thick absorbent paper then compared tothe color chart |
Tallquist scale |
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Wgat are the percentage error of Tallquist scale |
30%-50% |
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It consists of a glass plate and an eyepiece |
Dare Hemoglobinometer |
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How many does the dare Hemoglobinometer has |
20 to 30 % |
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It produces a true and relatively stable solution of hematin |
Alkaline solution |
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Disadvantage of alkaline hematin is that the blood of newborns and young infants contains ______. This may be significant source of errpr in hemoglobin determination whem using the alkaline hematin method |
Alkali - resistant fetal hemoglobin |
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A ___ is done by measuring oxyhemoglobin. It is simple and quick but there is no possibility of preparing a stable HbO2 standard |
Photometric determination |
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Used to measure pulse oxygen saturation and pulse rate |
Photoelectric oxyhemoglobin monitor |
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Not done routinely only when there is a presence of carbon monoxide poisoning |
Carboxyhemoglobin |
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Used in Van Slyke Oxygen Capacity to measure the amount of oxygen |
Van Slyke manometric apparatus |
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1 gm of hemogl9bin is equal to how many oxygen |
1.34mL |
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This method is used in screening potential blood donors |
Specific gravity method |
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The specific gravity of method employs ___ woth known specific gravity _____ |
Copper sulfate;1.052 and 1.054 |
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Specific gravity of blood |
1.053 |
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Two chemical methods of hemoglobin determination |
Kennedy's and Wong's |
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Chemical method pf hemoglobin is used to measure ___ |
Amount of iron present in the blood |
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One gram of hemoglobin carries ____ of iron |
3.47mg |
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Screening test for detecting variant of abnormal hemoglobin |
Hemoglobin electrophoresis |
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Hemoglobin electrophoresis uses ____ at an ___ |
Cellulose acetate ; alkaline pH (8.2 -8.6) |
