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25 Cards in this Set
- Front
- Back
Blood smear: fixative |
Methanol
|
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Blood smear: Acidic dye
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Eosin
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Blood smear: basic dye
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Methylene blue
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ID:
Nucleus is broken into segments |
Segmenter
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ID:
Cytoplasm contains small, pinkish granules |
Segmenter
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ID:
Nucleus is compact and usually round |
Lymphocyte
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ID:
Cytoplasm is light blue and scanty |
Lymphocyte
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ID:
Younger form of neutrophil with C, S, U or horse shoe-shaped nucleus |
Band
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ID:
Nucleus is spongy and sprawling with brain-like convolutions |
Monocyte
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ID:
Cytoplasm is gray, vacuoles sometimes present |
Monocyte
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ID:
Nucleus is usually bilobed |
Eosinophil
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ID:
Large coarse reddish or orange granules in cytoplasm |
Eosinophil
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ID:
Nucleus is indistinct and obscured by granules |
Basophil
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ID:
Cytoplasm contains large purplish-black or dark blue granules |
Basophil
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Conv Factor and Unit of Absolute Count:
CU -> SI |
Divide by 1000
Unit: x 10^9/L |
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Methods of counting fields (4)
|
Four-field Meander
Two-field Exaggerated Battlement Strip differential |
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Method of Staining:
Involves placing the blood smear on a rack positioned in a dish |
Staining Dish Method
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Automated Methods of Staining:
Freshly prepared staining solutions are used daily or every 4-8 hours during operation |
Hemasteiner Automatic Slide Stainer
|
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Automated Methods of Staining:
Stain-Pak is used |
Hema-Tek 1000 Slide Stainer
|
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Automated Methods of Staining:
Similar to Hema-Tek 1000, but with better pumps and volume controls |
Hema-Tek 2000 Slide Stainer
|
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Stains used in WBC Diff:
contains Methylene blue or its oxidative product like Azure B; considered polychromatic |
Romanowsky stain
|
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Stains used in WBC Diff:
Most satisfactory in general routine hematologic studies |
Wright's stain
|
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Stains used in WBC Diff:
Excelent stain for the demonstration of inclusion bodies and intracellular parasites aside from staining WBCs |
Giemsa stain
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Stains used in WBC Diff:
Similar to Wright's stain, except different oxidizing method |
Leishman, Jenner and May-Grunwald
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Stains used in WBC Diff:
Consists of Romanowsky stain and another dye |
Panoptic stain |