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25 Cards in this Set

  • Front
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Blood smear: fixative

Methanol
Blood smear: Acidic dye
Eosin
Blood smear: basic dye
Methylene blue
ID:
Nucleus is broken into segments
Segmenter
ID:
Cytoplasm contains small, pinkish granules
Segmenter
ID:
Nucleus is compact and usually round
Lymphocyte
ID:
Cytoplasm is light blue and scanty
Lymphocyte
ID:
Younger form of neutrophil with C, S, U or horse shoe-shaped nucleus
Band
ID:
Nucleus is spongy and sprawling with brain-like convolutions
Monocyte
ID:
Cytoplasm is gray, vacuoles sometimes present
Monocyte
ID:
Nucleus is usually bilobed
Eosinophil
ID:
Large coarse reddish or orange granules in cytoplasm
Eosinophil
ID:
Nucleus is indistinct and obscured by granules
Basophil
ID:
Cytoplasm contains large purplish-black or dark blue granules
Basophil
Conv Factor and Unit of Absolute Count:
CU -> SI
Divide by 1000
Unit: x 10^9/L
Methods of counting fields (4)
Four-field Meander
Two-field
Exaggerated Battlement
Strip differential
Method of Staining:
Involves placing the blood smear on a rack positioned in a dish
Staining Dish Method
Automated Methods of Staining:
Freshly prepared staining solutions are used daily or every 4-8 hours during operation
Hemasteiner Automatic Slide Stainer
Automated Methods of Staining:
Stain-Pak is used
Hema-Tek 1000 Slide Stainer
Automated Methods of Staining:
Similar to Hema-Tek 1000, but with better pumps and volume controls
Hema-Tek 2000 Slide Stainer
Stains used in WBC Diff:
contains Methylene blue or its oxidative product like Azure B; considered polychromatic
Romanowsky stain
Stains used in WBC Diff:
Most satisfactory in general routine hematologic studies
Wright's stain
Stains used in WBC Diff:
Excelent stain for the demonstration of inclusion bodies and intracellular parasites aside from staining WBCs
Giemsa stain
Stains used in WBC Diff:
Similar to Wright's stain, except different oxidizing method
Leishman, Jenner and May-Grunwald
Stains used in WBC Diff:
Consists of Romanowsky stain and another dye

Panoptic stain