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91 Cards in this Set
- Front
- Back
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Crenate |
Hypertonic |
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80-100 fL |
MCV ref range |
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26-34 pg |
MCH ref range |
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Swells and lyse |
Hypotonic solution |
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32-37 g/dL |
MCHC ref range |
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High MCHC |
Spherocytes mchc? |
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11.5-14.5 % |
RDW ref range |
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Anisocytosis |
RDW is directly proportional with? |
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Females- 36-46% Males- 41-53% |
Hct ref range |
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Hct |
%age of rbc in a given volume of whole blood |
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Spun microhct |
Reference manual method for hct |
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Mcv × rbc / 10 |
Hct formula |
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Males- 13.5-17.5 g/dL Females-12-16 g/dL |
Hgb ref range |
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150-450 x10^9 |
Plt ref range |
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6.8-10.2 fP |
MPV ref range |
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Wright's stain |
Most commonly used routine PBS stain |
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Methylene blue Eosin |
Composition of Wright's stain |
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Methanol fixative |
Used in the staining process to fix the cells to the slide |
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Add phosphate buffer (6.4-6.8 pH) |
How to start stain |
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Prussian blue |
Nonvital monochrome stain |
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K ferrocyanide HCl Safranin (counter) |
Prussian blue composition |
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Supravital stain |
New methylene blue (ppt RNA) Neutral red with BCG. (Counter) |
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Heinz bodies |
Neutral red with brilliant cresyl green visualizes what |
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G6PD |
Heinz bodies clinical disorder |
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Mesoblastic (yolk sac) Hepatic Myeloid/medullary phase |
3 stages of intrauterine hematopoiesis |
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Yolk sac/mesoblastic phase |
Begins at 19 days gestation |
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Erythroblast |
First cell to be produced |
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Portland, Gower I, Gower II |
Produced by erythroblast |
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6 week gestation |
Start of hepatic phase |
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Hgb F, A and A2 |
Hgb in hepatic phase |
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Myeloid/medullary phase |
5th month of gestation |
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Granulocytes |
Produced mainly in myeloid stage |
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Myeloid Lymphoid |
2 progenitor cells |
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CFU-MEG |
No IL3 |
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CFU-Ba |
No GM-CSF |
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Thrombopoietin, GM-CS Thrombocytes |
CFU-MEG |
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GM-CSF, G-CSF, IL3 Neutrophils |
CFU GM, CFU-G |
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GM-CSF, IL-3, M-CSF Monocyte |
CFU-GM CFU-M |
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Erythropoietin, IL3, GM-CSF Erythrocyte |
CFU-E |
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GM-CSF, IL3, IL5 Eosinophil |
CFU-Eo |
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IL3, IL4 Basophil |
CFU-Ba |
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Primary lymphoid tissue |
Ag-independent lymphopoiesis |
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Granulocytes, monocytes |
Phagocytes of the leukocytes |
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Lymphocytes, plasma cells and monocytes |
Immunocytes of leukocyte |
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4-11 x10^9 |
Ref range of WBC |
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Basophil Eo Neutro |
Granuloctes |
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Neutrophils |
First to reach the tissue and phagocytize bacteria (They die in the process) |
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Monocytes |
Differentiate into macrophage Do not die in the process |
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T-lymph |
Cellular immunity Produce cytokines and IL |
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B-lymph |
Develop into plasma cells and produce Ab; humoral immunity |
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Natural killer cells |
Large granular lymphocytes Destroy tumor cells and viruses |
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Eosinophils |
Modulate allergic response caused by basophil degranulation |
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Basophils |
Mediate immediate hypersensitivity rxns (type I, anaphylactic) |
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CD2, CD3 |
Lymphoid, pan T cells CD marker |
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CD4 |
Helper/inducer Tcells CD marker |
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CD8 |
Suppressor/T cytotoxic CD marker |
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CD13 |
Pan myeloid cd marker |
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CD11c, CD14 |
Monocytes CD marker |
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CD19, CD20 |
Lymphoid, pan B cells CD marker |
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CD 33 |
Pan myeloid cell CD marker |
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CD34 |
Stem cell maker |
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CD16, CD56 |
NK cells CD marker |
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Myeloblast |
Earliest recognizable granulocyte precursor |
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Promyelocyte |
Primary granules |
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Myeloperoxidase (azurophilic granules) |
What is contained in 1° granules |
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Myelocyte |
First stage where granulocyte types can be differentiated into eos, baso and neu |
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Myelocyte |
Has 2° granules; last stage capable of mitosis |
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Alkaline phosphatase, lysozyme, lactoferrin |
Contained in 2° granules |
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Myelocyte |
Has prominent golgi apparatus |
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Metamyelocyte |
Juvenile cell |
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<1/2 |
Nuclear indent in metamyelocyte |
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>1/2 |
Nuclear indent of band neutrophil |
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Necrosis |
Induced by extracellular forces such as lethal chemical, biological and physical events Blood cell is killed |
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Apoptosis |
Programmed cell death |
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Dohle bodies |
Small oval inclusions in cytoplasm stains light blue |
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Regenerative shift to the left |
Most common type of left shift |
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Leukemoid |
High LAP |
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CML |
Low LAP Philadelphia |
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Pathologic neutrophilia |
With shift to the left Response to tissue damage |
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Leukoerythroblastic reaction |
Immature leukocytes and immature erythrocytes in blood |
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Indirectly proportional |
Eo and ACTH |
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Chronic granulomatous disease |
Males to females 6:1 Oxidative respiratory pathway defect Inability to degranulate and bactericidal function Sex linked and autosomal |
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Chediak-Higashi syndrome |
Giant cytoplasmic granules Abnormal fusion of 1° and 2° neutrophilic granules autosomal recessive |
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Pelger huet anomaly |
Autosomal dominant Pince-nez Nucleus dumbbell or peanut shaped |
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May-Hegglin Anomaly |
Autosomal dominant Dohle-like inclusion Gray blue and cigar shaped Giant plt |
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Alder-reilly anomaly |
Autosomal recessive Azurophilic granules |
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Kupffer cells |
Monocytes in liver |
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Microglial cells |
Monocytes in CNS |
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Osteoclasts |
Monocyte in bones |
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Langerhan's cell |
Monocyte in skin |
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Alveolar cells |
Monocytes in lungs |
