H. Influenzae (Hib) And B. Pertussus

Front 1

major infection caused by Hib

Back 1

meningitis, epiglottitis, pneumonia, otitis media, cellulitis

note: meningitis and epiglottitis are medical emergencies

Front 2

major infection caused by pertusses

Back 2

whooping cough

Front 3

less common effet of Hib

Back 3

arthritis and osteomyelitis

Front 4

less ocmmon effect of b. pertussis

Back 4

pneumonia

Front 5

reservoir for Hib vs. b. pertussus

Back 5

humans

Front 6

transmission of hib vs. pertusses

Back 6

droplet

Front 7

Mophology of Hib vs. Pertusses

Back 7

Gm - coccobaccili

Front 8

growth factors for Hib

Back 8

X (hemin) and V (nad)

Front 9

growth factors fo B. pertussis

Back 9

Bordet-Gensou (glycerol, startch, blood)
fresh blood!!!

Front 10

metabolic needs of Hib vs. Pertusses

Back 10

faculatative anaerobe and strict aerobe

Front 11

carriers of the unencapsulated strain of Hib

Back 11

30-50% of children

Front 12

does Hib type be have exotoxin

Back 12

no

Front 13

four outcomes of Hib type B infection

Back 13

1. cellulitis to face, pyarthrosis to joints
2. nasopharyngisits, otisits media (initial onset)
3. epiglottitis
4. Bacteremia - meningitis

Front 14

the two outcomes of type B hib which are life threateneing

Back 14

epiglottis and meningitis

Front 15

agar to grow Hib type B

Back 15

chocolate agar

does not grow on fresh agar - the mild heating for chocolate agar releases X and V from red blood cells

Front 16

Hib type B might form what kind of colonies if in the presense of othe bacteria

Back 16

satellit colonies - the other bacteria cal lyse RBCs and release hemin (factor X)

Front 17

how many serotypes does type B Hib have

Back 17

6

Front 18

Quelling reaction is used to

Back 18

identify capsue - e.g for Hib type B

Front 19

what purpose does the Hib type B capsule serve

Back 19

anti-phagocytic

Front 20

used to be the most common cuase of bacterial meningitis

Back 20

Hib type B

Front 21

diagnosis of Hib type B

Back 21

1. history and age
2. blood/CSF
3. chocolate agar w/ co2
4. demonstration of need for X and V
5. type B capsular Ag in CSF by immunofluorescence or immunoelectrophoresis
6. latex agglutinanins

Front 22

treatment of meningitis caused by Hib type B

Back 22

ampicillan, third generation cephalosporins, augmentin (for ampicillin resistant strains)

Front 23

prophylaxis for contact Hib type B

Back 23

rifampin, single dose of ciprofloxacin

Front 24

non-typeable H. influenzae cause what

Back 24

otitis media in kids
pneumonia

Front 25

peritrichous pili

Back 25

in non-typeable Hi helps organism adhere to respiratory mucous

Front 26

vaccine for non-typeable Hi

typeable?

Back 26

no vaccine for non-typeable but there is a vaccine for typeable - linked with the diptheria toxiod

Front 27

variation in what leades to very little immunity in non-typable Hi strains

Back 27

OM proteins

Front 28

two growth factors, both present in blood which is needed by typeable (encapsulated) H.Influenzae

Back 28

X (heat statble) and V (heat labile)

Front 29

3 routes of pathogenesis of non-typeable Hi1

Back 29

1. macropinocytosis
2. paracytosis
3. LPS bindign to Paf

Front 30

what is paracytosis

Back 30

can pass between cells to invade sub epith space

Front 31

treatment of otitis media caused by non-typeable Hi

Back 31

amoxicillin or augmenten

Front 32

treatment for meningitis caused by non-typeable Hi

Back 32

ampicillin and chloramphenicl or 3rd generation cephalosporins

Front 33

profalaxis for Hib - nontypeable

Back 33

rifampin

Front 34

a haemophilus pathogen which is a STD and causes chancroid (ragged ulcer on genetils)

Back 34

D. ducreyi

Front 35

a haemophilus pathogen that causes purulent conjunctivitis in hot clines and is called koch-weeks bacillus

Back 35

H. aegypticus

Front 36

a haemophilus pathogen which causes pharyngitis and bacterial endocarditis

Back 36

H. parainfluenzae

Front 37

four colonization factors for bordetella pertussis

Back 37

1. pilli - attaches res. cilia
2. FHA - hemagglutinin -
3. pertactin
4. tracheal colonization factor

Front 38

what catarrhal stage in pertussis is marked by

Back 38

mild respiratory symptoms

Front 39

the paroxysmal stage is marked by what

Back 39

acute inflammation of trachea, bronchi, bronchioles and paroxysnal cough (1-4 wks)

Front 40

toxin which works by ADP ribosylating a G protein which normally inhibits adenylate cyclase. this disinhibitis adenylate cyclase and increase camp activity

Back 40

pertussis toxin

compare w/ cholera toxin which also results in an increase in cAMP

Front 41

impairs leukocyte function and causes cell death by increase adenylate cyclase

Back 41

calmodulin stimulated adenylate cyclase

Front 42

pertussis causes what symptoms

Back 42

lympohcytosis, sensitization to histimine and increase insulin secretion

Front 43

pertussis toxin which causes vascular sm muscle contraction and ischemic necosis of lung tissue

Back 43

dermonectoric toxin - (mouse lethal, toxin, health labile)

Front 44

inhibits DNA synthesis and kills trachael epithelial cells (ciliostatis)

Back 44

Tracheal cytotoxin secreted by B. pertusis

Front 45

Bordetella pertussis
gm?
needs for growing

Back 45

gm -
needs fresh blood media
is aerobic

Front 46

immunity against B. pertussis

Back 46

passive is not adequate - ab after several weeks of cough

Front 47

diagnosis of B. pertussis

Back 47

1. isolation by nasopharyngeal swab
2. gram stain
3. direct fluorescent labeled Ab test

Front 48

treatment for B. pertussis

Back 48

erythromycin - not penicillin or ampicillin