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8 Cards in this Set
- Front
- Back
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First dye used? |
Crystal violet dye. All cells stained blue/purple |
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Second step is to add Iodine solution. Which acts as what? |
As a mordant and forms a crystal-violet-iodine complex |
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After adding mordant cells stained of what colour? |
They remain blue |
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After the mordant what is added? |
An organic solvent (ethanol or acetone) |
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After the organic solvent what is colour of the cells? |
Gram +ve remain blue Gram -ve becomes colourless |
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What is the last dye used for gram staining? |
Red dye: safranin: stains gram -ve red. |
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Gram +ve bacteria under gram staining is seen as what color? |
Blue |
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Name few bacteria that cannot be stained by gram staining |
Mycobacteria-acid fast Treponema pallidum- dark field microscopy Mycoplasma-no cell wall Chylamidia and ricketsiae- intracellular |
