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24 Cards in this Set

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What is the best way to differentiate between Campylobacter and Helicobacter
Presence of Flagellar Sheets
Camp - Absent
Heli - Present
What is required for Campylobacter growth
5 -10% O2
5 - 10% CO2
80 - 90% N2
What shaped and Gram Stain are Campylobacter
Curved Spiral, motile/unsheathed flagella, gram -
What is the metabolism of Campylobacter
Nonfermentative/nonoxidative

Derive energy from the use of amino acids and 4-6-carbon intermediates of the TCA
What diseases are campylobacter associated with
gastroenteritis, abortions in cows, sheep, humans, sepsis, meningitis, periodontal disease and proctitis (homosexual)
What is the habitat of Campylobacter
Campylobacters are normal flora in GI tracts
What is a good test to differentiate between Camplyobacter coli and Campylobacter jejuni
Test Hippurate
C. coli Hippurate -
C. jejuni Hippurate +
How many types of transmission are there for Campylobacter
2 exogenous and endogenous
Exogenous Transmission
acquire from handling infected animals or through the fecal-oral route
Endogenous Transmission
Transmission from mother to fetus through genitourinary tract
What are pathogenic factors of Campylobacter
LPS - protects from stomach acid
Flagellar Motion/Shape - passes through mucous membrane
Distending Cytotoxin -stops cell divisioin
Enterotoxin - similar to cholera, ETEC, and LT
Adhesins - attachment
Symptoms of Campylobacter infection
watery diarrhea, bloody diarrhea, extraintestinal disease
80% of patients are asymptomatic
Diseases caused by campylobacter
Acute Enteritis, Appendicitis like syndrome, Bacteremia, septic abortion, Reactive arthiritis
Lab Idnetification of campylobacter
From stool: Campy-Thio, Campy-BAP in 5% O2, 10% CO2, 85% N2
What is Campy-BAP?
10% Sheep's Blood, Vancomycin (inhibits Gram +), trimethoprim (e. coli), polymyxin B, cephalothin, amphotericin B (fungi), in Brucella Agar base
What is the most important pathogen of campylobacter/ what does it causes
C. jejuni
Isolated from stoll 7 times more than Salmonella shigella
Guillain-Barre syndrome
Treatment for Campylobacter
usually just fluid and electrolyte replacement.
If antibiotics: erythromycin and quinolones
_______ causes peptic ulcers
Helicobacter
Growth environment contains _____, _____, and ______ at _____ temperature
10% CO2, 5% O2, 85% N2
35-37 C, 42 C
High Humidity
_____cytotoxin in H. pylori causes _____
Vacuolating, cellular damage
_____ causes activates signal transudction.

This can cause ____
Cag A
cellular growth, maybe cancer
H. pylori can be cultured on :_______ + 5% Sheep Blood
________+ 7% Horse Blood
________ + antibitic
Mueller Hinton
Brain Heart Infusion
Thayer-Martin
___ and helps protect H. pylori by hydrolizing Urea to __ and __.
Urease
ammonia
CO2
_____, _____, and _____ are treatments for H. pylori
Bismuth, Amoxicillin, and Tetracyclin