Gram Negative Bacteria

Front 1

What type of E.coli if found in GI tract and non pathogenic but can cause infection in compromised patients

Back 1

Commensal E.coli

Front 2

What type of E.coli are pathogenic group of diarrheal E.coli that cause infection in GI tract

Back 2

Diarrheagenic E.coli

Front 3

What type of pathogenic group cause extraintestinal infection?

Back 3

Extra intestinal E.coli

Front 4

Heat labile enterotoxins (LT) and heat stable toxin (St) belongs to which E.coli

Back 4

Entero toxigenice E.coli
ETEC

Front 5

Form aggregative adherence on tissues/cells

Back 5

Entero aggregative E.coli
EAEC

Front 6

Adhere to intestinal mucosa and cause extensive host cell actin rearrangement belong to which E.coli

Back 6

Entero pathogenic E.coli
EPEC

Front 7

Primary cause of hemorrhagic colitis (HC) or bloody diarrhea --> HUS belong to which E.coli

Back 7

Entero Hemorrhagic E.coli
EHEC STEC

Front 8

Which E.coli is similar to EPEC except it produce shiga toxins (Stx)

Back 8

EHEC hemorrhagic

Front 9

Which E.coli closely resemble shigella but do not form shiga toxinx (STX)

Back 9

EIEC
Entero invasive E.coli

Front 10

Which E,coli cause invasive, dysenteric form of diarrhea

Back 10

EIEC
entero invasive E,coli

Front 11

Which E.coli forms a diffuse adherent pattern to tissues/cells?

Back 11

Diffusely adherent E.coli
DAEC

Front 12

Not associated with foodborne outbreak yet

Back 12

Diffusely adherent E.coli
DAEC

Front 13

Which bacteria is GRAM -ve rod of enterobacteriaceae family

Back 13

E.coli O157 : H7

Front 14

Natural habitat of E,coli

Back 14

Intestinal tract of cattle

Front 15

Virulence factors of E.coli

Back 15

Shiga like toxins 1 2
Intimin
Tir

Front 16

Site of infection of E.coli O157 : h7

Back 16

Large intestine

Front 17

Mechanisms of E.coli bacteria

Back 17

Adherence: E.coli's intimin bind to Tir on the epithelial cells --> colonize --> AE lesion

Front 18

E.coli O157:h7 produce?

Back 18

Shiga like toxins Stx 1 and Stx 2

Front 19

How shiga toxins work ?

Back 19

Bind to receptor on epithelial cells
Once internalized, Stx toxins inhibit protein synthesis ---> host cell death ---> bloody diarrhea

Front 20

Disease of E,coli O157 : h7

Back 20

Bloody diarrhea -----> hemorrhagic colitis
Kidney failure- HUS

Front 21

Both E EC and E EC cause AE lesions and are invasive resulting in bloody diarrhea?

Back 21

EPEC EHEC

Front 22

EHEC is more virulent due the production of ?

Back 22

Shiga like Toxin (STX)

Front 23

E EC and E EC are not invasive but can produce enterotoxins which lead to watery diarrhea

Back 23

ETEC EAEC

Front 24

what 3 methods of detection of E.coli O157: H7

Back 24

1.biochemical / cultural methods
2.Immunological methods
3.Molecular methods

Front 25

what color of colonies appear on sorbitol MacConkey agar for E.coli O157:H7

Back 25

White
other E.coli - pink
(biochemical methods)

Front 26

E.coli 0157 :H7 are lactose positive , sorbitol negative and beta glucuronidase negative

Back 26

Correct
E.coli O157 :H67

Front 27

O antigen code for

Back 27

Somatic /LPS
E.coli O157 :H67

Front 28

H antigen code for

Back 28

Flagella
E.coli O157 :H67

Front 29

Fecal.............of agricultural lands by domesticated and wild animals

Back 29

Adulteration
E.coli O157 :H67

Front 30

Gram -ve rods of Enterobacteriaceae family that do not ferment lactose

Back 30

Salmonella

Front 31

Important foodborne Salmonella serovars

Back 31

S.enterica serovar Typhimurium , Enteritidis, New port , stanley
Salmonella

Front 32

Serovars of Salmonella based on ?

Back 32

O,H and K antigens
K antigen : capsule

Front 33

How Salmonella spread?
3 methods

Back 33

Carriers
Animal Feeds
Fecal/water

Front 34

Enteristis (infection at small intestine)

Back 34

Salmonella

Front 35

Most frequent isolates

Back 35

S.enterica serovar Typhimurium

Front 36

what type of serovar bacteria found in poultry and eggs

Back 36

S.enterica serovar Enteritidis
Poultry and eggs

Front 37

Salmonella are reclassified into which 2 species based on DNA

Back 37

S.bongori
S. enterica

Front 38

4 important Salmonella serovars

Back 38

Typhimurium
Enteritidis
Newport
Stanley

Front 39

Cell surface ruffling in which 2 bacteria?

Back 39

Salmonella Typhimurium and shigella spp

Front 40

How do cell surface ruffling work?

Back 40

inject virulence genes in host
Entry through ruffling
Multiply and spread in host cells
Cause apoptosis of host cells

Front 41

WhAT are injected through type III secretion system (TTSS)

Back 41

Effectors
Salmonella

Front 42

Bacteria that do cell ruffline

Back 42

Salmonella and shigella
E.coli NO ruffling, only assist

Front 43

Which bacteria is Gram-ve rods non motile.
most strains are non - lactose fermentor

Back 43

Shigella

Front 44

Which are the 2 common species of Shigella

Back 44

S.sonnei
s. flexneri

Front 45

Which bacteria can be foodborne and non foodborne

Back 45

Shigella

Front 46

What type of bacteria are involved in Food contaminated due to poor personal hygiene or fecal oral transmission

Back 46

shigella

Front 47

What type of bacteria are involved in food that are served

Back 47

shigella

Front 48

Which bacteria is the 3rd largest foodborne monitored by foodnet

Back 48

shigella

Front 49

How the pathogenesis of Shigella works?

Back 49

Invade epithelial cells of small or large intestine through ruffling
Multiply intra cellularly
Spread from cell to cell by actin-- based motility like listeria

Front 50

Which bacteria mediated cell ruffling and internalization?

Back 50

Salmonella

Front 51

Which bacteria mediated induction of apoptosis

Back 51

shigella

Front 52

Shigella mediated induction of apoptosis produce?

Back 52

Shiga toxins that are thermostable and inhibit protein synthesis.

Front 53

Other virulence factors of Shigella mediated induction of apoptosis?

Back 53

Apoptosis of macrophages

Front 54

Which gram- rods bacteria of enterobacteriacease family are motile at 25, but not 37?

Back 54

Yersinia Enterocolitica

Front 55

Common serotypes of Yersinia Enterocolitica

Back 55

O:3 and O:8

Front 56

which bacteria may involved in other illness such as reactive arthirtis (ReA) and thyroid disorders?

Back 56

Yersinia Enterocolitica

Front 57

Which bacteria is an pathogenic invasive m/o?

Back 57

Yersinia Enterocolitica

Front 58

how pathogenesis of Yersinia Enterocolitica works?

Back 58

YadA an adhesin that binds to host integrin on M cells in small intestine
Adhere and invade host cells
Heat stable enterotoxin, YsT
local and systemic inflammation

Front 59

Which bacteris is gram negative spirally curved small rods: motile

Back 59

Campylobacter jejuni

Front 60

What are grow in our lumen which needed only 3-6% oxygen and 10% CO2

Back 60

microaerophilic
Campylobacter jejuni

Front 61

Pathogenesis of Campylobacter jejuni

Back 61

May produce enterotoxins
recruit white blood cells/inflammation
induce host cell death
may lead to autoimmune disease

Front 62

Virulence factor for Campylobacter jejuni

Back 62

Thermostable direct hemolysin TDH
Cholera toxin
Adherence factors: fimbriae, hemagglutinins

Front 63

Vibrio cholerae produces what kind of toxin in the lumen?

Back 63

Cholera Toxin (CT)
Campylobacter jejuni

Front 64

How vibrio work?

Back 64

Cells attach to M cells by toxin co-regulated pili (TCP)
Produce CT which induces cAMP and results in water secretion in lumen.
CT subunit A1 activates adenylate cyclase through ADP- ribosylation

Front 65

What are the 4 type of pathogenesis of intracellular bacteria?

Back 65

1.Adherence/attachment
2.Entry/invasive
3.Growth and spreading
4.Toxin/ enzyme

Front 66

which 4 types of Gram negative pathogens used Type III secretion system ( TTSS)

Back 66

1.E.coli O157:H7
2.Salmonalle
3.Shigella
3.yersinia